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An Attempt to Determine the Origin of DNA Single-Strand Breaks Observed after Treatment of Mammalian Cells with Alkylating Agents

  • A. Abbondandolo
  • E. Dogliotti
  • P. H. M. Lohman
  • F. Berends

Abstract

Upon exposure of DNA to alkylating agents a number of different lesions are produced, either directly or as a consequence of secondary modifications of the original alkylation. We have investigated the formation of one of these second lesions, single-strand DNA breaks (ssb), after treatment of cultured Chinese hamster ovary cells with four different ethylating agents: N-ethyl-N’-nitro-N-nitrosoguanidine (ENNG), N-ethyl-N-nitrosourea (ENU), ethylmethanesulfonate (EMS), and diethyl sulfate (DES).

Keywords

Chinese Hamster Ovary Cell Alkaline Hydrolysis Lysis Time Repair Event Diethyl Sulfate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1983

Authors and Affiliations

  • A. Abbondandolo
    • 1
  • E. Dogliotti
    • 2
  • P. H. M. Lohman
    • 3
  • F. Berends
    • 3
  1. 1.Istituto di Mutagenesi e Differenziamento CNRPisaItaly
  2. 2.lstituto Superiore di SanitàRomeItaly
  3. 3.Medical Biological Laboratory TNORijswijkThe Netherlands

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