An Attempt to Determine the Origin of DNA Single-Strand Breaks Observed after Treatment of Mammalian Cells with Alkylating Agents
Upon exposure of DNA to alkylating agents a number of different lesions are produced, either directly or as a consequence of secondary modifications of the original alkylation. We have investigated the formation of one of these second lesions, single-strand DNA breaks (ssb), after treatment of cultured Chinese hamster ovary cells with four different ethylating agents: N-ethyl-N’-nitro-N-nitrosoguanidine (ENNG), N-ethyl-N-nitrosourea (ENU), ethylmethanesulfonate (EMS), and diethyl sulfate (DES).
KeywordsChinese Hamster Ovary Cell Alkaline Hydrolysis Lysis Time Repair Event Diethyl Sulfate
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