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Transfer and Detection of Proteins on Membrane Supports

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Protein Analysis and Purification

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Abstract

Electrotransferring proteins from a gel to a membrane, known as Western blotting, combines the resolution capabilities of electrophoretic protein separation with the specificity of immunological identification in a rapid and highly sensitive format. For optimal results, the macromolecules must be transferred efficiently from the gel to the membrane. The proteins which are now immobilized on the membrane can be identified and visualized by using specific and sensitive immunobiochemical detection techniques.

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Authors and Affiliations

  1. Massachusetts General Hospital, Jackson 715, G.I. Unit, 02114, Boston, MA, USA

    Ian M. Rosenberg

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  1. Ian M. Rosenberg
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© 1996 Springer Science+Business Media New York

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Rosenberg, I.M. (1996). Transfer and Detection of Proteins on Membrane Supports. In: Protein Analysis and Purification. Birkhäuser, Boston, MA. https://doi.org/10.1007/978-1-4757-1108-0_7

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  • DOI: https://doi.org/10.1007/978-1-4757-1108-0_7

  • Publisher Name: Birkhäuser, Boston, MA

  • Print ISBN: 978-0-8176-3665-4

  • Online ISBN: 978-1-4757-1108-0

  • eBook Packages: Springer Book Archive

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