Getting Started with Protein Purification

  • Ian M. Rosenberg


Protein purification is usually a multi-step process exploiting a wide range of biochemical and biophysical characteristics of the target protein, such as its source, relative concentration, solubility, charge, and hydrophobicity. The ideal purification strives to obtain the maximum recovery of the desired protein, with minimal loss of activity, combined with the maximum removal of other contaminating proteins.


Protein Solution Ammonium Sulfate Protein Purification DEAE Cellulose Dialysis Tubing 
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Copyright information

© Springer Science+Business Media New York 1996

Authors and Affiliations

  • Ian M. Rosenberg
    • 1
  1. 1.Massachusetts General HospitalBostonUSA

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