Since the pioneering separation of human serum proteins, albumin, α-, β- and γ- globulin carried out by Tiselius in 1937, electrophoretic separation of biologically active molecules has been an essential technique in biomedical research. As an analytical tool, electrophoretic characterization of biomolecules has progressed tremendously since the time of Tiselius, becoming more sophisticated, specialized and useful as new types of electrophoretic systems have developed. Today, gel electrophoresis is arguably the single most powerful analytical technique in use. Electrophoresis provides a method for simultaneously analyzing multiple samples or for analyzing multiple components in a single sample. As will become apparent, polyacrylamide gel electrophoresis (PAGE) is a pivotal procedure in protein characterization in the sense that analytical reactions probing the structure and composition of the target protein are carried out before and after the separation of a complex mixture of macromolecules by PAGE. A wealth of information can be obtained using PAGE. Molecular weight determination, purity of proteins, post-translational modifications, subunit structure, enzyme activity, protein processing, and amino acid sequence are just a few areas that can be investigated using PAGE technology.
KeywordsCoomassie Brilliant Blue Electrophoretic Technique Cathode Buffer Plate Assembly Acrylamide Solution
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