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Kinins—II pp 83-95 | Cite as

Methods for the Determination of Glandular Kallikrein by Means of a Chromogenic Tripeptide Substrate

  • Egil Amundsen
  • J. Putter
  • P. Friberger
  • M. Knos
  • M. Larsbraten
  • G. Claeson
Part of the Advances in Experimental Medicine and Biology book series (AEMB)

Abstract

A chromogenic peptide substrate H-D-Val-Leu-Arg-pNA (S-2266) has been used for the determination of glandular kallikrein derived from pancreas, urine and saliva. The conditions used have been optimized. The methods developed are simple and shown to have good reproducibility.

Keywords

Procedure Volume Urinary Kallikrein Kallikrein Activity Initial Rate Method Glandular Kallikrein 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Amundsen, E., Svendsen, L., VennerOd, A.M. and Laake, K., 1974, Determination of plasma kallikrein with a new chromogenic tripeptide derivative, in:Chemistry and Biology of the Kallikrein-kinin system in Health and Disease, eds. J.J.Pisano and K.F. Austen, Fogarty International Center Proceedings No. 27, U.S. Government Printing Office, Washington D.C., p. 215.Google Scholar
  2. Claeson, G., Amundsen, E. et al., To be published, Chromogenic peptide substrates for kallikrein.Google Scholar
  3. Claeson, G., Friberger, P., Knös, M. and Ericsson, E., 1978, Methods for determination of prekallikrein in plasma, glandular kallikrein and urokinase, Haemostasis 7, p. 76.PubMedGoogle Scholar
  4. Geiger, R., Personal communications.Google Scholar
  5. Pisano, J.J., 1975, Chemistry and biology of the kallikrein-kinin system, in: Proteases and Biological Control, eds. E. Reich, D.B. Rifkin and E. Shaw, Cold Spring Harbor Laboratory, p. 199.Google Scholar

Copyright information

© Springer Science+Business Media New York 1979

Authors and Affiliations

  • Egil Amundsen
    • 1
  • J. Putter
    • 2
  • P. Friberger
    • 3
  • M. Knos
    • 3
  • M. Larsbraten
    • 1
  • G. Claeson
    • 3
  1. 1.Institte for Surgical ResearchRikshospitalet, OsloNorway
  2. 2.Institute for PharmacokineticsBayer AGWuppertalGermany
  3. 3.Peptide ResearchAB KABIMolndalSweden

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