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Kinins—II pp 487-497 | Cite as

Studies on the Effects of Bradykinin and Its Fragments on the Central Nervous System

  • K. Kariya
  • H. Okamoto
  • H. Iwaki
  • A. Hashimoto
  • M. Yagyu
  • Y. Tsuchiya
  • Y. Okada
Part of the Advances in Experimental Medicine and Biology book series (AEMB)

Abstract

Kininases were separated by DEAE-cellulose column chromatography with stepwise gradient of sodium chloride from rat whole brain. F-A which developed with 70 mM NaC1 cleaved Phe-Ser bond, F-B with 100 mM NaC1 hydrolyzed post-proline site and F-C with 150 mM NaC1 broke down bradykinin to amino acids. F-A activity was inhibited by Arg-Pro-Pro-Gly-Phe, Ser-Pro-Phe-Arg and Cly-Phe-Ser-Pro, and the two formers blocked F-B activity.

Keywords

Rabbit Brain Dansyl Chloride Stepwise Gradient Dansylated Amino Acid Pharmacological Significance 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1979

Authors and Affiliations

  • K. Kariya
    • 1
  • H. Okamoto
    • 1
  • H. Iwaki
    • 1
  • A. Hashimoto
    • 1
  • M. Yagyu
    • 2
  • Y. Tsuchiya
    • 2
  • Y. Okada
    • 2
  1. 1.Department of Pharmacology Faculty of Pharmaceutical SciencesKobe-Gakuin Univ.Igawadani-cho, Tarumi-ku, Kobe 673Japan
  2. 2.Department of Chemistry Faculty of Pharmaceutical SciencesKobe-Gakuin Univ.Igawadani-cho, Tarumi-ku, Kobe 673Japan

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