Advertisement

Kinins—II pp 393-407 | Cite as

Effect of Bradykinin to Cyclic AMP Levels and Response of Murine Lymphocytes

  • Yoshitami Kimura
  • Takeo Fujihira
  • Kazutomi Kato
  • Masaichi Furuya
  • Masahiko Onda
  • Akiro Shirota
Part of the Advances in Experimental Medicine and Biology book series (AEMB)

Abstract

Ho information is available on the pharmacological effect of bradykinin to lymphocytes and immunological responses of them. In this study it was clarified that bradykinin as well as histamine elevated cyclic adenosine 3′,5′monophosphate (cAMP) levels of murine splenic or lymph node lymphocytes and mature thymocytes (cortisone-resistant thymus), but did not increase cAMP levels of immature thymocytes as well as histamine. The increased cAMP ratios in T cell-enriched splenic lymphocytes by the impulse of bradykinin were higher than that in splenic and lymph node lymphoid cells by the stimulation of bradykinin. It was also demonstrated that bradykinin as well as histamine suppressed DNA synthesis by mitogenic (PHA-P, Con-A) stimulation of splenic lymphocytes, but not a effect to the response of lymphocytes by mitogenic (LPS) stimulation was observed. These facts suggest that bradykinin may play an important role in the regulation of immunologic lymphocyte responses.

Keywords

Lymphoid Cell cAMP Level Splenic Lymphocyte Cyclic Monophosphate Increase cAMP Level 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Abell, C. W. and T. M. Monahan, 1973, The role of adenosine 3′, 5′-cyclic monophosphate in the regulation of mammalian cell division, J. of Cell Biol. 59, 549.CrossRefGoogle Scholar
  2. Bach, M. A., 1975, Differences in cyclic AMP changes after stimulation by prostaglandins and isopreterenol in lymphocyte sub-populations, J. Clin. Inv. 35, 1074.Google Scholar
  3. Ballet, J. J. and E. Merler, 1976, The separation and reactivity in vitro of a separation of a subpopulation of human lymphocytes which bind histamine. Correlation of histamine reactivity with cellular maturation, Cellular Immunol. 24, 250.Google Scholar
  4. Beising-Schneider, R. and M. Hang, 1976, Role of cyclic AMP on differentiation of T- and B-lymphocytes during the immune induction, Cellular Immunol. 27, 121.CrossRefGoogle Scholar
  5. Brown, B. L., J. D. M. Albano, R. P. Ekins and A. M. Sgherzi, 1971, A simple and sensitive saturation. assay method for the measurement of adenosine 3:5’-cyclic monophosphate, Biochem. J. 121, 561.Google Scholar
  6. Burne, H. R., L. M. Lichtenstein, K. L. Melmon, C. S. Henney, Y. Weinstein and C. M. Shearer, 19 74, Modulation of inflammation and immunity by cyclic AMP, Science 184, 19.Google Scholar
  7. Cross, M. E. and M. G. Ord, 1971, Changes in histone phosphorylation and associated early metabolic events in pig lymphocyte cultures transformed by phytohaemagglutinin or 6-N, 2′0 dibutyryl-adenosine 3′,5′-cyclic monophosphate, Biochem. J. 124, 241.Google Scholar
  8. De Ruberts, F. R., T. V. Zenser, W. H. Adler and T. Hudson, 1974, Role of cyclic adenosine 3′,5′-monophosphate in lymphocyte mitogenesis, 113, 151.Google Scholar
  9. Gilman, A. C., 1970, A protein binding assay for adenosine 3′,5′- cyclic monophosphate, Proc.Natl. Acad. Sci, 67, 305.Google Scholar
  10. Hadden, J. W., E. M. Hadden, M. K. Haddox and N. D. Goldberg, 1972, Guanosine 3′,5′cyclic monophosphate: a possible intracellular mediator of mitogenic influences in lymphocytes, Proc. Nat. Acad. Sci. 69, 3024.Google Scholar
  11. Henney, C. S. and L. M. Lichtenstein, 1971, The role of cyclic AMP in the cytolytic activity of lymphocytes, J. Immunol. 107, 610.PubMedGoogle Scholar
  12. Julius, M. E., E. Simpsone and L. A. Herzberg, 1973, A rapid method for the isolation of functional thymus-derived murine lymphocytes, Eur. J. Immunol. 3, 645.Google Scholar
  13. Lichtenstein, L. M. and E. Gilespie, 1973, Inhibition of hista-mine release by histamine controlled by H2 receptor, Nature 244, 287.PubMedCrossRefGoogle Scholar
  14. MacManus, J. P. and J. F. Whitfield, 1970, Stimulation of DNA synthesis and mitotic activity of thymic lymphocytes by cyclic adenosine 31,5’-monophosphate, Exp. Cell Res. 58, 188.Google Scholar
  15. Makman, M. H. and M. I. Klein, 1972, Expression of adenylate cyclase, catecholamine receptor, and cyclic adenosine monophosphate-dependent protein kinase in synchronized culture of Chang’s liver cells, Proc. Natl. Acad. Sci. 69, 456.Google Scholar
  16. Novogrodsky, A. and E. Katchalski, 1970, Effect of phytohemagglutinin and prostaglandins on cyclic AMP synthesis in rat lymph node lymphocytes, Biochem. Biophys. Acta. 215, 291.Google Scholar
  17. Plaut, M., L. M. Lichtenstein, E. Gillespie and C. S. Henney, 1973, Studies on the mechanism of lymphocyte-mediated cytolysis, IV. Specificity of the histamine receptor on effector T cells, J. Immunol, 111, 389.Google Scholar
  18. Rocklin, R. E., 1976, Modulation of cellular-immune responses in vivo and in vitro by histamine receptor-bearing lymphocytes, J. Clin. Invest, 57, 1051.Google Scholar
  19. Roszkowski, W., M. Plaut and L. M. Lichtenstein, 1976, Selective display of histamine receptors on lymphocytes, Science 195, 683.CrossRefGoogle Scholar
  20. Smith, J. W., A. L. Steiner, W. M. Newberry and C. W. Parker, 1971, Cyclic adenosine 3′,5′-monophosphate in human lymphocytes, Alterations after phytohemagglutinin stimulation, J. Clin. Invest. 50, 432.Google Scholar
  21. Smith, J. W., A. L. Steiner and C. W. Parker, 1971, Human lymphocyte metabolism. Effects of cyclic and noncyclic nucleotides on stimulation by phytohemagglutinin, J. Clin. invest. 50, 442,Google Scholar
  22. Sullivan, T. J., K. L. Parker, W. Stenson and C. W. Parker, 1975, Modulation of cyclic AMP in purified rat mast cells, T. Responses to pharmacologie, metabolic, and physiol stimuli, J. Immunol. 114, 1473.Google Scholar
  23. Teh, H.-S. and V. Paetkau, 1976, Regulation of immune responses, I. Effects of cyclic AMP and cyclic GMP on immune induction, Cellular Immunol. 24, 209.Google Scholar
  24. Vischer, T. L., 1976, The differential effect of cyclic AMP on lymphocyte stimulation by T- or B-cell mitogens, Immunol. 30, 735.Google Scholar
  25. Wang, S. R. and B. Zweiman, 1978, Histamine suppression of human lymphocyte responses to mitogens, Cellular Immunol. 36, 28.CrossRefGoogle Scholar
  26. Watson, J., 1976, The involvement of cyclic nucleotide metabolism in the initiation of lymphocyte proliferation induced by mito-gens, J. Immunol. 117, 1656.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1979

Authors and Affiliations

  • Yoshitami Kimura
    • 1
  • Takeo Fujihira
    • 1
  • Kazutomi Kato
    • 1
  • Masaichi Furuya
    • 1
  • Masahiko Onda
    • 2
  • Akiro Shirota
    • 2
  1. 1.Department of Microbiology and ImmunologyNippon Medical SchoolTokyo 113Japan
  2. 2.Department of 1st SurgeryNippon Medical SchoolTokyo 113Japan

Personalised recommendations