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Kinins—II pp 325-333 | Cite as

Purification of Horse Renal Kallikrein and Chemical Relations with Horse Urinary Kallikrein

  • G. Porcelli
  • G. B. Marini-Bettolo
  • H. R. Croxatto
  • M. Di Jorio
Part of the Advances in Experimental Medicine and Biology book series (AEMB)

Abstract

Kallikrein was purified from horse kidney by several steps of chromatographic procedure and by affinity chromatography on SepharoseConcanavaline. Horse urinary kallikrein was previously purified by DE-32 hydroxylapatite and by Sephadex G-100 gel filtration. On the purified final sample of renal and urinary kallikrein the aminoacid composition and the gel electrophoretic molecular weight were determined. The ratio in uMoles between each aminoacid residue of both hydrolyzed renal and urinary kallikrein of horse is about 1,00 ± 0,30. Except for. Pro, 1/2Cys and basic aminoacid residues a good proportion was obtained. It is confirmed that the different molecular weight, respectively 47,500 for renal kallikrein and 28,000 for the urinary enzyme is an artefact of the different procedures used for the purification of horse kallikrein.

Keywords

Esterase Activity Acetone Powder Good Proportion Urinary Enzyme Aminoacid Residue 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1979

Authors and Affiliations

  • G. Porcelli
    • 1
    • 2
  • G. B. Marini-Bettolo
    • 1
    • 2
  • H. R. Croxatto
    • 1
    • 2
  • M. Di Jorio
    • 1
    • 2
  1. 1.Centro Chimica Recettori C.N.R., Instituto di Chimica Fac. Med.Universita CattolicaRomeItaly
  2. 2.Laboratorio di FisiologiaUniversidad Catolica Santiags de ChileChile

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