Advertisement

Kinins—II pp 305-312 | Cite as

A Simple, Large Scale Process for Purifying Human Urinary Kallikrein, Based on Reverse Osmosis and Ammonium Sulfate Precipitation

  • Amintas F. S. Figueiredo
  • Marcos Mares-Guia
Part of the Advances in Experimental Medicine and Biology book series (AEMB)

Abstract

A simple, large scale process for the purification of human urinary kallikrein is described which is based upon concentration by reverse osmosis, ammonium sulfate precipitation, and gel filtration on a column of Sephadex-G-150. The yield in protein is higher than any reported in the literature to this date; the purified enzyme seems to be identical to that reported by Figueiredo and Mares-Guia at the Klein-Symposium in Paris, 1978, based on the procedure of Hial, et al., (1974).

Keywords

Ammonium Sulfate Reverse Osmosis Human Urinary Amino Acid Analysis Ammonium Sulfate Precipitation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Arens, A., and G.L. Haberland (1977). Determination of kallikrein activity in animal tissue using biochemical methods. In: G.L. Haberland, J.W. Rohen, and T. Suuki (eds.) Kininogenases. Kallikrein 4. Physiological properties and Pharmacological rationale. F.K. Schattauer Verlag, Stuttgart-New York, pp. 43–53.Google Scholar
  2. Figueiredo, A.F.S., and M. Mares-Guia (1978). Some properties of human urinary kallikrein obtained through scaled-up purification. In: Current Concepts in Kinin Research. Ed. C.L. Haberland. Pergamon Press, 1978. In Press.Google Scholar
  3. Geiger, R., K. Mann, and T. Bettels (1977). Isolation of human urinary kallikrein by affinity chromatography. J. Clin. Chem. Clin. Biochem. 15: 479–483.PubMedGoogle Scholar
  4. Hial, V., C.R. Diniz, and Mares-Guia (1974). Purification and properties of a human urinary kallikrein (kininogenase). Biochemistry 13: 4311–4318.PubMedCrossRefGoogle Scholar
  5. Laemmli, U.K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685.PubMedCrossRefGoogle Scholar
  6. Lowry, 0.H., M.J. Rosenbrough, A.F. Lewis, and R.J. Randall (1951). Protein measurement with the folin phenol reagent. J. Biol. Chem. 193: 265–275.Google Scholar
  7. Matsuda, Y., K. Miyazaki, H. Moriya, Y. Fujimoto, Y. Hojima, and C. Moriwaki (1976). Studies on urinary kallikreins. I. Purification and characterization of human urinary kallikreins. J. Biochem. 80: 671–679.PubMedGoogle Scholar
  8. Moriya, H., J.V. Pierce, and M. Webster (1963). Purification and some properties of three kallikreins. Ann. N.Y. Acad. Sci. 104: 172–185.CrossRefGoogle Scholar
  9. Narendra, B.O., and J.W. Ryan (1978). A simple high-yield procedure for isolation of human urinary kallikreins. Biochem. J, 171: 285–288.Google Scholar
  10. Prado, E.S. and J.L. Prado (1961). Partial purification of a plasma kinin forming enzyme from horse urine. Experientia (Basel) 17: 31–33.CrossRefGoogle Scholar
  11. Yoi, 0.0., K.F. Austen, and J. Spragg (1977). Kinin-generating and esterolytic activity of purified human urinary kallikrein (urokallikrein). Biochem. Pharmacol. 26: 1893–1900.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1979

Authors and Affiliations

  • Amintas F. S. Figueiredo
    • 1
  • Marcos Mares-Guia
    • 1
  1. 1.Depto. De Bioquimica, Inst. Cien. Biol.Universidade Federal De Minas Gerais30.000 Belo HorizonteBrazil

Personalised recommendations