A Simple, Large Scale Process for Purifying Human Urinary Kallikrein, Based on Reverse Osmosis and Ammonium Sulfate Precipitation
A simple, large scale process for the purification of human urinary kallikrein is described which is based upon concentration by reverse osmosis, ammonium sulfate precipitation, and gel filtration on a column of Sephadex-G-150. The yield in protein is higher than any reported in the literature to this date; the purified enzyme seems to be identical to that reported by Figueiredo and Mares-Guia at the Klein-Symposium in Paris, 1978, based on the procedure of Hial, et al., (1974).
KeywordsAmmonium Sulfate Reverse Osmosis Human Urinary Amino Acid Analysis Ammonium Sulfate Precipitation
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