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Kinins—II pp 273-290 | Cite as

Purification and Characterization of Hog Pancreatic Kallikrein I, II and III

  • Heizo Kira
  • Seiji Hiraku
  • Hiroshi Terashima
Part of the Advances in Experimental Medicine and Biology book series (AEMB)

Abstract

Three forms of kallikrein [EC 3.4.4.21] were purified from hog pancreas powder by affinity chromatography on bovine lung trypsin inhibitor-Sepharose column and DEAE-Sephadex A-50 column chromatography. Each form of kallikrein was tentatively. named II, III and I, in the order of elution on DEAE-Sephadex A-50 column. Final yields of I, II and III, per kg of the starting material, were 6.7, 7.6 and 1.9 mg, respectively. Kallikrein I and II were crystallized.

Keywords

Soybean Trypsin Inhibitor Ammonium Acetate Buffer Vasodilator Activity Urea Treatment Chloromethyl Ketone 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Andrews, P., 1964, Estimation of the molecular weights of proteins by Sephadex gel-filtration, Biochem. J., 91, 222.PubMedGoogle Scholar
  2. Blix, G. 1948, The determination of hexosamines according to Elson and Morgan, Acta Chem. Stand., 2, 467.CrossRefGoogle Scholar
  3. Cuatrecasas, P., 1970, Protein purification by affinity chromatography, J. Biol. Chem., 245, 3059.Google Scholar
  4. Dubois, M., K. A. Gilles, J. K. Hamilton, P. A. Rebers and F. Smith, 1956, Colorimetric method for determination of sugars and related substances, Anal. Chem., 28, 350Google Scholar
  5. Edelhoch, H., 1967, Spectroscopic determination of tryptophan and tyrosine in proteins, Biochemistry, 7, 1948Google Scholar
  6. Fiedler, F., 1976, Pig pancreatic kallikreins A and B, in: Methods in Enzymology, Vol. 45, eds L. Lorand ( Academic Press, New York ) P. 289.Google Scholar
  7. Fiedler, F. and E. Werle, 1967, Vorkommen zweier Kallikreinogene im Schweinepankreas und Automation der Kailikrein– und Kallikreinogenbestimmung, Hoppe-Seyler’s Z. Physiol. Chem., 348, 1087.Google Scholar
  8. Fiedler, F. and E. Werle, 1968, Activation, inhibition, and pH dependence of the hydrolysis of α-N-benzoyl-L-arginine ethyl ester catalyzed by kallikrein from porcine pancreas, Europ. J. Biochem., 7, 27.Google Scholar
  9. Fiedler, F., C. Hirschauer and E. Werle, 1970, Anreicherung von Praallikrein B aus Schweinepankreas und Eigenschften vershiedener Formen des Pankreaskallikreins.Google Scholar
  10. Fiedler, F., C. Hirschauer and E. Werle, 1975, Characterization of pig pancreatic kallikrein A and B, Bd. 356, 1879Google Scholar
  11. Kassel’, B., 1970, Bovine trypsin-kallikrein inhibitor, in: Methods in Enzymology, Vol. 19, eds G. E. Perlmann and L. Lorand ( Academic Press, New York ) P. 844.Google Scholar
  12. Kunitz, M., 1947, Crystalline soybean trypsin inhibitor. II. General properties, J. Gen. Physiol., 30, 291.Google Scholar
  13. Kutzbach, C. and G. Schmidt-Kastner, 1972, Kallikrein from pig pancreas. Purification, separation of components A and B, and crystallization, Hoppe-Seyler’s Z. Physiol. Chem., 353, 1099.Google Scholar
  14. Lowry, O. H., N. J. Rosebrough, A. L. Farr and R. J. Randall, 1951, Protein measurement with the Folin phenol reagent, J. Biol. Chem., 193, 265.Google Scholar
  15. Moore, S., 1963, The determination of cysti e as cysteic acid, J. Biol. Chem., 238, 235.Google Scholar
  16. Moriwaki, C., Y. Hojima and H. Moriya, 1974, A proposal-use of combined assays of kallikrein activity measurement, Chem. Pharm. Bull. ( Tokyo ), 22, 975.Google Scholar
  17. Spackman, D. H., W. H. Stein and S. Moore, 1958, Anal. Chem.,30, 1190. Takami, T., 1969 a, Purification of hog pancreatic kallikreins and their proteolytic activities, Seikagaku (Tokyo), 41, 777.Google Scholar
  18. Takami, T., 1969 b, Action of hog pancreatic kallikrein on synthetic substrates and its some other enzymatic properties, J. Biochem. ( Tokyo ), 66, 651.Google Scholar
  19. Vestererberg, O. and H. Svensson, 1966, Isoelectric fractionation, analysis, and characterization of ampholytes in natural pH gradients, Acta Chem. Scand., 20 820.Google Scholar
  20. Weber, K. and M. Osborn, 1969, The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis, J. Biol. Chem., 244, 4406.Google Scholar
  21. Williams, D. E. and R. A. Reinsfold, 1964, Disk electrophoresis in polyacrylamide gels, Ann. N. Y. Acad. Sci., 121, 373.Google Scholar
  22. Zuber, M. and E. Sache, 1974, Isolation and characterization of porcine pancreatic kallikrein, Biochemistry, 13, 3098.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1979

Authors and Affiliations

  • Heizo Kira
    • 1
  • Seiji Hiraku
    • 1
  • Hiroshi Terashima
    • 1
  1. 1.Research InstituteOno Pharmaceutical Co., Ltd.Sakurai, Shimamoto-cho, Mishima-gun, Osaka 618Japan

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