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Kinins—II pp 173-184 | Cite as

Assay Methods for Prekallikrein and Kininogens and Their Applications

  • Yasuhiro Uchida
  • Sachiko Oh-Ishi
  • Kunio Tanaka
  • Yoshiteru Harada
  • Akinori Ueno
  • Makoto Katori
  • Shuji Funahashi
  • Takenori Hashimoto
  • Takami Ueno
  • Junzo Kodama
Part of the Advances in Experimental Medicine and Biology book series (AEMB)

Abstract

Prekallikrein activity in plasma was assayed using a synthetic peptidyl fluorogenic substrate (carbobenzoxy-L-phenylalanyl-L-arginine 4-methylcoumarinyl-7-amide), after activation of prekallikrein by acetone and kaolin. For total kininogen assay, the pretreatment of plasma at pH 2.0 was the best to eliminate bradykinin potentiators and kininase activity, before addition of trypsin to convert kininogen to bradykinin. Assay method of high molecular weight (HMW) kininogen was established by conversion of HMW-kininogen to bradykinin through activation of Hageman factor by glass powder and that of low molecular weight (LMW) kininogen was also by treatment of HMW-kininogen-depleted plasma in the same way as that for total kininogen.

The marked reduction of prekallikrein and HMW-kininogen, not of LMW-kininogen, was found in pleural fluid of rat carrageenin pleurisy, and in plasma after i.v. injection of bromelain in rats. Members of the pedigree of hereditary angioneurotic edema patients also show low levels of prekallikrein and kininogens in plasma.

Keywords

Assay Method Pleural Fluid Glass Powder Plasma Kallikrein Hageman Factor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Springer Science+Business Media New York 1979

Authors and Affiliations

  • Yasuhiro Uchida
    • 1
  • Sachiko Oh-Ishi
    • 1
  • Kunio Tanaka
    • 1
  • Yoshiteru Harada
    • 1
  • Akinori Ueno
    • 1
  • Makoto Katori
    • 1
  • Shuji Funahashi
    • 2
  • Takenori Hashimoto
    • 2
  • Takami Ueno
    • 2
  • Junzo Kodama
    • 2
  1. 1.Department of PharmacologyKitasato University School of MedicineSagamihara 228Japan
  2. 2.Osaka National HospitalOsaka 540Japan

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