Method for Measurement of Human Urinary Kininase Activity
A method was developed to measure kininase activity in human urine. The method consists of dialysis of human centrifuged urine sample against phosphate buffer and partial fractionation of A-50 Sephadex column. The enzymatic property of urinary kininase, which destroys bradykinin when incubated, is estimated from its effect on a definite amount of bradykinin, using rat uterus.
KeywordsUrinary Sample Human Urine Enzymatic Property Phosphate Solution Tyrod Solution
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