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Reversible Absorbance Changes and Modulation of Biological Activities by Blue Light

  • Helga Ninnemann
Part of the NATO Advanced Science Institutes Series book series (NSSA, volume 68)

Abstract

For nearly half a century, carotene (carotenoproteins) and flavin (flavoproteins) have been discussed as prime candidates for photoreceptors of blue light-regulated biological processes (Bünning, 1937; Galston and Baker, 1949; Galston, 1977). With time carotenoids have lost their predominant candidacy — so far, in fact, that recently an advocatus diaboli had to step up to defend its case (Shropshire, 1980). This fate is unjust, however, since the evidence for a photoreceptor role of flavins in many blue light reactions doesn’t necessarily eliminate possible carotenid photoreceptors in others. We might have to give up the concept of one common blue light photoreceptor or even of one single compound in the class of carotenoids or flavins. For the time being we should confine ourselves to individual photoreceptors for individual blue light reactions. It is true that flavins/ flavoproteins have gained attention as photoreceptors for a wide variety of blue light responses in slime molds, fungi, algae, higher plants and even insects (Fig. 1, Ninnemann, 1980). I am inclined to add the action spectrum for induction of carotenoid biosynthesis in Neurospora by deFabo(1976) to this series (Fig. 2) which because of its small action maximum in the near uv resembles a flavin more than a carotenoid though it was not interpreted this way originally. Since Ed deFabo is present in this auditorium, I suppose we will have a second advocatus diaboli in a moment.

Keywords

Methylene Blue Nitrate Reductase Glutamine Synthetase Action Spectrum Absorbance Change 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Aparicio, P.J. Roldân, J.M. and Calero, F., 1976, Blue light photo-reactivation of nitrate reductase from green algae and higher plants, Biochem. Biophys. Res. Commun. 70: 1071–1077CrossRefGoogle Scholar
  2. Aslam, M., Oaks, A. and Huffaker, R.C., 1976, Effect of light and glucose on the induction of nitrate reductase and on the distribution of nitrate in etiolated barley leaves, Plant Physiol., 58: 588–591CrossRefGoogle Scholar
  3. Berns, D.S. and Vaughn, J.R., 1970, Studies on the photopigmanL system in Phycomyces, Biochem. Biophys. Res. Commun., 39: 1094–1103Google Scholar
  4. Brain, R.D., Woodward, D.O. and Briggs, W.R., 1977a, Correlative studies of light sensitivity and cytochrome content in Neurospora crassa, Carnegie Yearbook, Wash., 76: 295–299Google Scholar
  5. Brain, R.D., Freeberg, J.A., Weiss, C.V. and Briggs, W.R., 1977b, Blue light-induced absorbance changes in membrane fractions from corn and Neurospora, Plant Physiol., 59: 948–952CrossRefGoogle Scholar
  6. Britz, S.J.,Schrott, E., Widell, S., Brain, R.D. and Briggs, W.R., 1977, Methylene blue-mediated red-light photoreduction of cytochromes in particulate fractions of corn and Neurospora, Carnegie Yearbook, Wash., 76: 289–293Google Scholar
  7. Britz, S.J., Schrott, E. Widell, S. and Briggs, W.R., 1979, Red light-induced reduction of a particle-associated b-type cytochrom from corn in the presence of methylene blue, Photochem. Photobiol., 29: 359–365CrossRefGoogle Scholar
  8. Bünning, E., 1937, Phototropismus und Carotinoide, Planta 26: 719–736CrossRefGoogle Scholar
  9. DeFabo, E., Harding, R.W. and Shropshire, W., 1976, Action spectrum between 260 and 800 nm for the photoinduction of carotinoid biosyn- thesis in Neurospora crassa, Plant Physiol. 57: 440–445CrossRefGoogle Scholar
  10. Delbrück, M., Katzir, A. and Presti, D., 1976, Responses of Phycomylowest triplet state of ces indicating optical excitation of the riboflavin, Proc. Natl. Acad. Sci. Wash.73: 1969–1973CrossRefGoogle Scholar
  11. Edmondson, D.E., Barman, B. and Tollin, G., 1972, On the importance of the N-5 position in flavin coenzymes. Properties of free and protein-bound 5-deaza analogs, Biochem. 11: 1133–1138CrossRefGoogle Scholar
  12. Erlanger, B.F., 1976, Photoregulation of biologically active macromolecules, Ann. Rev. Biochem.45: 267–283CrossRefGoogle Scholar
  13. Frisell, W.R., Chung, C.W. and Mackenzie, C.G., 1959, Catalysis of oxidation of nitrogen compounds by flavin coenzymes in the presence of light, J. Biol. Chem. 234: 1297–1302Google Scholar
  14. Galston, A.W., 1950, Riboflavin, light and the growth of plants, Science 111: 619–624CrossRefGoogle Scholar
  15. Galston, A.W., 1977, Riboflavin retrospective or deja-vu in blue, Photochem. Photobiol. 25: 503–504CrossRefGoogle Scholar
  16. Galston, A.W. and Baker, R.S., 1949, Studies on the physiology of light action. II. The photodynamic action of riboflavin, Amer. J. Bot. 36: 773–780CrossRefGoogle Scholar
  17. Garrett, R.H. and Nason, A., 1967, Involvement of a b-type cytochrome in the assimilatory nitrate reductase of Neurospora crassa, Proc. Natl. Acad. Sci. Wash.58: 1603–1610CrossRefGoogle Scholar
  18. Goldsmith, M.H., Caubergs, R.J. and Briggs, W.R., 1980, Light-inducible cytochrome reduction in membrane preparations from corn coleoptiles, Plant Physiol. 66: 1067–1073CrossRefGoogle Scholar
  19. Gressel, J., 1980, Blue light and transcription, in “The Blue Light Syndrome”, H. Senger, ed., Springer-Verlag Berlin, Heidelberg, New York, p. 133–153Google Scholar
  20. Hemmerich, P., 1976, The present status of flavin and flavocoenzyme chemistry, Progress in the Chemistry of Organic Natural Products 33: 451–527Google Scholar
  21. Hug, D.H., 1981, Photoactivation of enzymes, in “Photochem. Photobiol. Rev. 6”,K.C. Smith, ed., Plenum Press, New York and London, p. 87–138CrossRefGoogle Scholar
  22. Jesaitis, A.J., 1974, Linear dichroism and orientation of the Phycomyces photopigment, J. Gen. Physiol. 63: 1–21CrossRefGoogle Scholar
  23. Jesaitis, A.J., Heners, P.R., Hertel, R. and Briggs, W.R., 1977, Characterization of a membrane fraction containing a b-type cytochrome, Plant Physiol. 59: 941–947CrossRefGoogle Scholar
  24. Johnson, J.L., Hainline, B.E. and Rajagopalan, K.V., 1980, Characterization of the molybdenum cofactor of sulfite oxidase, xanthine oxidase and nitrate reductase, J. Biol. Chem. 255: 1783–1786Google Scholar
  25. Jones, R.W. and Sheard, R.W., 1977, Effects of blue and red light on nitrate reductase level in leaves of maize and pea seedlings, Plant Sci. Lett. 8: 305–311CrossRefGoogle Scholar
  26. Klemm-Wolfgramm, 1979, Korrelation zwischen Absorptionsänderungen und physiologischer Wirkung von Blaulicht bei Neurospora - Nitratreduktase als möglicher Photorezeptor, Doctoral Thesis, Tübingen.Google Scholar
  27. Klemm, E. and Ninnemann, H., 1978, Correlation between absorbance change and a physiological response induced by blue light in Neuro-spora, Photochem. Photobiol. 28: 227–230CrossRefGoogle Scholar
  28. Klemm, E. and Ninnemann, H., 1979, Nitrate reductase - a key enzyme in blue light-promoted conidiation and absorbance change of Neurospora, Photochem. Photobiol.29: 629–632CrossRefGoogle Scholar
  29. Klemm-Wolfgramm, E. and Ninnemann, H., 1979, Influence of various nitrogen sources and sugar concentrations on light-inducible and rhythmic conidiation of Neurospora crassa mutant albino-band, Microsensory Newsletters 1,3: 16–18Google Scholar
  30. Lang-Feulner, J. and Rau, W., 1975, Redox dyes as artificial photoreceptors in light-dependent carotenoid synthesis, Photochem. Photobiol. 21: 179–183CrossRefGoogle Scholar
  31. Leong, T.Y. and Briggs, W.R., 1981, Partial purification and characterization of a blue light-sensitive cytochrome-flavin complex from corn membranes, Plant Physiol. 67: 1042–1046CrossRefGoogle Scholar
  32. Lewis, S.C., Schiff, J.A. and Epstein, H.T., 1961, Photooxidation of cytochromes by a flavoprotein from Euglena, Biochem. Biophys. Res. Commun. 5: 221–225CrossRefGoogle Scholar
  33. Lipson, E.D. and Presti, D., 1977, Light-induced absorbance changes in Phycomyces photomutants, Photochem. Photobiol. 25: 203–208CrossRefGoogle Scholar
  34. Manabe, K. and Poff, K.L., 1978, Purification and characterization of the photoreducible b-type cytochrome from Dictyostelium discoideum, Plant Physiol. 61: 961–966CrossRefGoogle Scholar
  35. McCormick, D.B., 1977, Interactions of flavins with amino acid resi-dues: assessments from spectral and photochemical studies, Photochem. Photobiol. 26: 169–182CrossRefGoogle Scholar
  36. Montagnoli, G., 1976, Light regulation of enzyme activity, in “Proc. Intl. School Biophys. of Photoreceptors and Photobehavior of Microorganisms”, G. Colombetti, ed., p. 219–258Google Scholar
  37. Munoz, V. and Butler, W.L, 1975, Photoreceptor pigment for blue light in Neurospora crassa, Plant Physiol. 55: 421–426CrossRefGoogle Scholar
  38. Munoz, V., Brody, S. and Butler, W.L., 1974, Photoreceptor pigment for blue light responses in Neurospora crassa, Biochem. Biophys. Res. Commun. 58: 322–327CrossRefGoogle Scholar
  39. Nicholas, D.J.D. and Nason, A., 1954, Mechanism of action of nitrate reductase from Neurospora, J. Biol. Chem. 211: 183–197Google Scholar
  40. Nicholas, J.C., Harper, J.E. and Hageman, R.H., 1976, Nitrate reductase activity in soybeans (Glycine max ( L) Merr.), Plant Physiol. 58: 731–735CrossRefGoogle Scholar
  41. Ninnemann, H., 1980, Blue light photoreceptors, BioScience 30: 166–17(CrossRefGoogle Scholar
  42. Ninneman, H., 1982, Photoreduction of cytochrome b557 of partially purified Neurospora nitrate reductase via its internal flavin, Photochem. Photobiol.35: 391–398CrossRefGoogle Scholar
  43. Ninnemann, H. and Klemm, E., 1976, Blue light induced absorption changes in plants and animals, abstr. 112, Plant Physiol. Supp. 57Google Scholar
  44. Ninnemann, H. and Klemm-Wolfgramm, E., 1980, Blue light-controlled conidiation and absorbance change in Neurospora are mediated by nitrate reductase, in “The Blue Light Syndrome”, H. Senger, ed., Springer-Verlag Berlin, Heidelberg, New York, p. 238–243Google Scholar
  45. Ninnemann, H., Strasser, R. and Butler, W.L., 1977, The superoxide anion as an electron donor to the mitochondrial electron transport chain, Photochem. Photobiol.26: 41–47CrossRefGoogle Scholar
  46. Otto, M.K.,Jayaram, M., Hamilton, R.M. and Delbrück, M., 1981, Replacement of riboflavin by an analogue in the blue-light photoreceptor of Phycomyces, Proc. Natl. Acad. Sci. Wash., 78: 266–269CrossRefGoogle Scholar
  47. Pan, S.-S. and Nason, A., 1978, Purification and characterization of homogeneous assimilatory reduced nicotinamide adenine dinucleotide phosphate nitrate reductase from Neurospora crassa, Biochim. Biophys. Acta523: 297–313CrossRefGoogle Scholar
  48. Penzer, G.R., 1970, The chemistry of flavins and flavoproteins: aerobic photochemistry, Biochem. J. 116: 733–743Google Scholar
  49. Poff, K.L., Butler, W.L. and Loomis jr., W.F., 1973, Light-induced absorbance changes associated with phototaxis in Dictyostelium, Proc. Natl. Acad. Sci. Wash., 70: 813–816CrossRefGoogle Scholar
  50. Poff, K.L. and Butler, W.L., 1974, Absorbance changes induced by blu€ light in Phycomyces blakesleeanus and Dictyostelium discoideum, Nature 248: 799–801CrossRefGoogle Scholar
  51. Poff, K.L. and Butler, W.L., 1975, Spectral characterization of the photo-reducible b-type cytochrome of Dictyostelium discoideum, Plant Physiol. 55: 427–429CrossRefGoogle Scholar
  52. Roldân, J.M. and Butler, W.L., 1980, Photoactivation of nitrate reductase from Neurospora crassa, Photochem. Photobiol. 32: 375–381CrossRefGoogle Scholar
  53. Roldân, J.M., Calero, F. and Aparicio, P.J., 1978, Photoreactivation of spinach nitrate reductase: role of flavins, Zeitschr. Pflanzenphysiol. 90: 467–474Google Scholar
  54. Rowell, P., Sampaio, M., Ladha, J.K. and Stewart, W.D., 1979, Alteration of Cyanobacterial glutamine synthetase activity in vivo in response to light and NH4, Arch. Microbiol. 120: 195–200CrossRefGoogle Scholar
  55. Sargent, M.L. and Briggs, W.R., 1967, The effects of light on a circa- dian rhythm of conidiation in Neurospora, Plant Physiol. 42: 1504–1510CrossRefGoogle Scholar
  56. Sargent, M.L. and Kaltenborn, S.H., 1972, Effects of medium composition and carbon dioxide on circadian conidiation in Neurospora, Plant Physiol. 50: 171–175CrossRefGoogle Scholar
  57. Schmidt, W. and Butler, W.L., 1976a, Flavin-mediated photoreactions in artificial systems: a possible model for the blue-light photoreceptor pigment in living systems, Photochem. Photobiol. 24: 71–75CrossRefGoogle Scholar
  58. Schmidt, W. and Butler, W.L., 1976b, Light-induced absorbance changes in cell-free extracts of Neurospora crassa, Photochem. Photobiol.24: 77–80CrossRefGoogle Scholar
  59. Schmidt, W., Thomson, K. and Butler, W.L., 1977, Cytochrom b in plasma membrane enriched fractions from several photoresponsive organisms. Photochem. Photobiol. 26: 407–411CrossRefGoogle Scholar
  60. Shropshire jr., W., 1980, Carotenoids as primary photoreceptors in blue-light responses, in “The Blue Light Syndrome”, H. Senger, ed., Springer-Verlag Berlin, Heidelberg, New York, p. 172–186Google Scholar
  61. Song, P.S., 1980, Spectroscopic and photochemical characterization of flavoproteins and carotenoproteins as blue light photoreceptors, in “The Blue Light Syndrome”, H. Senger, ed., Springer-Verlag Berlin, Heidelberg, New York, p. 157–171Google Scholar
  62. Song, P.S., Moore, T.A. and Sun, M., 1972, Excited states of some plant pigments, in “The Chemistry of Plant Pigments”, C.O. Chichester, ed., Academic Press. New York, p. 33–74Google Scholar
  63. Sorger, G.J. and Davies, J., 1973, Regulation of nitrate reductase of Neurospora at the level of transcription and translation, Biochem. J. 134: 673–685Google Scholar
  64. Stoy, V., 1955, Action of different light qualities on simultaneous photosynthesis and nitrate assimilation in wheat leaves, Physiol. Plant. 8: 963–986CrossRefGoogle Scholar
  65. Subramanian, K.N., Padmanaban, G. and Sarma, P.S., 1968, The regulation of nitrate reductase and catalase by amino acids in Neurospora crassa, Biochim. Biophys. Acta151: 20–32CrossRefGoogle Scholar
  66. Swader, J.A., Stocking, C.R. and Lin, C.H., 1975, Light-stimulated absorption of nitrate by Wolffia arrhiza, Physiol. Plant. 34: 335–341CrossRefGoogle Scholar
  67. Tischner, R. and Hüttermann, A., 1978, Light-mediated activation of nitrate reductase in synchronous Chlorella, Plant Physiol. 62: 284–286CrossRefGoogle Scholar
  68. Tischner, R. and Hüttermann, A., 1980, Regulation of glutamine synthetase by light and during nitrogen deficiency in synchronous Chlorella sorokiniana, Plant Physiol. 66: 805–808CrossRefGoogle Scholar
  69. Tischner, R. and Lorenzen, H., 1979, Nitrate uptake and nitrate reduction in synchronous Chlorella, Planta 146: 287–292CrossRefGoogle Scholar
  70. Traber, R., Kramer, H. and Hemmerich, P., 1982, Mechanism of light-induced reduction of biological redox centers by amino acids, Biochem. 21: 1687–1693CrossRefGoogle Scholar
  71. Widell, S. and Björn, L.O., 1976, Light-induced absorption changes in etiolated coleoptiles, Physiol. Plant. 36: 305–309CrossRefGoogle Scholar
  72. Wu, T., MacKenzie, R.E. and McCormick, D.B., 1970, Kinetics and mechanism of oxidation-reduction reactions between pyridine nucleotides and flavins, Biochem. 9: 2219–2224CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1983

Authors and Affiliations

  • Helga Ninnemann
    • 1
  1. 1.Institut für Chemische PflanzenphysiologieUniversität Tübingen74 TübingenFederal Republic of Germany

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