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Gene Structure, Organisation and Expression of the Light-Harvesting B800-850 α and β Polypeptides in Photosynthetic Bacteria

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Molecular Biology of Membrane-Bound Complexes in Phototrophic Bacteria

Part of the book series: FEMS Symposium ((FEMSS))

Abstract

Members of the phototrophic non-sulfer purple bacteria produce ATP either by oxidative phosphorylation when growing aerobically in the dark or by photophosphorylation when grown anaerobically in the light. During the differentiation of the photosynthetic intracytoplasmic membrane, three pigment-protein complexes, besides other membrane bound complexes and cofactors, are synthesized. These are the photochemical reaction center (RC) and the light-harvesting antenna complexes B870 (LHI) and B800-850 (LHII). The light-harvesting complexes absorb light energy which is then directed to the photochemical reaction center with high efficiency.

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References

  • Armstrong, G.A., Alberti, M., Leach, F., and Hearst, J.E., 1989, Nucleotide sequence, organization, and nature of the protein products of the carotenoid biosynthesis gene cluster of Rhodobacter capsulatus, Mol. Gen. Genet. 216: 254–268.

    Article  PubMed  CAS  Google Scholar 

  • Brunisholz, R.A., Cuendet, P.A., Theiler, R., and Zuber, H., 1981, The complete amino acid sequence of the single light harvesting protein from chromatophores of Rhodospirillum rubrum G-9, FEBS Lett. 129: 150–154.

    Article  CAS  Google Scholar 

  • Chen, C.Y., Beatty, J.T., Cohen, S.N., and Belasco, J.G., 1988, An intercistronic stem-loop structure functions as an mRNA decay terminator necessary but insufficient for put mRNA stability, Cell 52: 609–629.

    Article  PubMed  CAS  Google Scholar 

  • Firsow, N.N., and Drews, G., 1977, Differentiation of the intracytoplasmic membrane of Rhodopseudomonas palustris induced by variations of oxygen partial pressure or light intensity, Arch. Microbial. 115: 299–306.

    Article  CAS  Google Scholar 

  • Hayashi, H., Nakano, M., and Morita, S., 1982a, Comparative studies of protein properties and bacteriochlorophyll contents of bacteriochlorophyll-protein complexes from spectrally different types of Rhodopseudomonas palustris, J.Biochem. 92: 1805–1811.

    PubMed  CAS  Google Scholar 

  • Hayashi, H., Miyao, M., and Morita, S., 1982b, Absorption and fluorescence spectra of light-harvesting bacteriochlorophyll-protein complexes from Rhodopseudomonas palustris in the near-infrared region, J. Biochem. 91: 1017–1027.

    PubMed  CAS  Google Scholar 

  • Kiley, P.J., and Kaplan, S., 1987, Cloning, DNA sequence, and expression of the Rhodobacter sphaeroides light-harvesting B800–850-a and B800–85043 genes, J. Bacteriol. 169: 3268–3275.

    PubMed  CAS  Google Scholar 

  • Klug, G.T., Kaufmann, N., and Drews, G., 1985, Gene expression of pigment-binding proteins of the bacterial photosynthetic apparatus: transcription and assembly in the membrane of Rhodopseudomonas capsulata, Proc. Natl. Acad. Sci. USA 82: 6485–6489.

    Article  PubMed  CAS  Google Scholar 

  • Lee, J.K., Kiley, P.J., and Kaplan, S., 1989, Posttranscriptional control of puc operon expression of B800–850 light-harvesting complex formation in Rhodobacter sphaeroides, J. Bact. 171: 3391–3405.

    PubMed  CAS  Google Scholar 

  • McClure, W.R., 1985, Mechanism and control of transcription initiation in prokaryotes, Ann. Rev. Biochem. 54: 171–204.

    Article  PubMed  CAS  Google Scholar 

  • Platt, T., 1986, Transcription termination and the regulation of gene expression, Annu. Rev. Biochem. 55: 339–372.

    Article  PubMed  CAS  Google Scholar 

  • Schumacher, A., and Drews, G., 1978, The formation of bacteriochlorophyll-protein complexes of the photosynthetic apparatus of Rhodopseudomonas capsulata during early stages of development, Biochim. Biophys. Acta 501: 183–194.

    Article  PubMed  CAS  Google Scholar 

  • Schumacher, A., and Drews, G., 1979, Effects of light intensity on membrane differentiation in Rhodopseudomonas capsulata, Biochim. Biophys. Acta 547: 417–428.

    Article  PubMed  CAS  Google Scholar 

  • Shine, J., and Dalgarno, L., 1974, The 3’-terminal sequence of Escherichia Coli 16S ribosomal RNA: Complementary to nonsense triplets and ribosomes binding sites, Proc. Natl. Acad. Sci. USA 71: 1342–1346.

    Article  PubMed  CAS  Google Scholar 

  • Shiozawa, J.A., Cuendet, P.A., Drews, G., and Zuber, H., 1980, Isolation and characterization of the polypeptide components from light-harvesting pigment-protein complex B800–850 of Rhodopseudomonas capsulata, EurJ.Biochem. 111: 455–460.

    Article  CAS  Google Scholar 

  • Tadros, M.H., Zuber, F., and Drews, G., 1982, The polypeptide components from the light-harvesting pigment-protein complex II (B800–850) of Rhodopseudomonas capsulata, Eur.JBiochem. 127: 315–318.

    Article  CAS  Google Scholar 

  • Tadros, M.H., and Waterkamp, K., 1989, Multiple copies of the coding regions for the light-harvesting B800–850 a and ß polypeptides are present in the Rhodopseudomonas palustris genome, EMBO J. 5: 1303–1308.

    Google Scholar 

  • Theiler, R., Suter, F., Wiemken, V., and Zuber, H., 1984, The light-harvesting polypeptides of Rhodopseudomonas sphaeroides R-26.1 I.Isolation, purification and sequence analysis, Hoppe-Seyler’s J. Physiol.Chem. 365: 703–719.

    Article  CAS  Google Scholar 

  • Tichy, H.V., Oberlé, Béatrice, Stiehle, H., Schiltz, E., and Drews, G., 1989, Genes downstream from PUCB and PUCA are essential for the formation of the B800–850 complex of Rhodobacter capsulatus, J. Bacteriol.,in press.

    Google Scholar 

  • Vagra, A.R., and Staehlin, L.A., 1985, Pigment-protein complexes from Rhodopseudomonas palustris: Isolation, characterization, and reconstitution into liposomes, J. Bacteriol. 161: 921–927.

    Google Scholar 

  • Wechsler, T.D., Brunisholz, R.A., Frank, G., Suter, F., and Zuber, H., 1987, The complete amino acid sequence of the antenna polypeptide B806–866–3 from the cytoplasmic membrane of the green bacterium Chloroflexus aurantiacus, FEBS Leu. 210: 189 – 194.

    Article  CAS  Google Scholar 

  • Whittenbury, R., and Mc Lee, A.G., 1967, Rhodopseudomonas palustris and Rhodopseudomonas viridis. Photosynthetic bacteria, Arch. Mikrobiol. 59: 324–334.

    CAS  Google Scholar 

  • Youvan, D.C., and Ismail, S., 1985, Light-harvesting II (B800–850 complex) structural genes from Rhodopseudomonas capsulata, Proc. Natl. Acad. Sci. USA 82: 58–62.

    Article  PubMed  CAS  Google Scholar 

  • Zhu, Y.S., Cook, D.N., Leach, F., Armstrong, G.A., Alberti, M., and Hearst, J.E., 1986, Oxygen-regulated mRNAs for light-harvesting and reaction center complexes and for bacteriochlorophyll and carotenoid biosynthesis in Rhodobacter capsulatus during the shift from anaerobic to aerobic growth, J. Bacteriol. 168: 1180–1188.

    PubMed  CAS  Google Scholar 

  • Zhu, Y.S., and Hearst, J.E., 1986, Regulation of the expression of the genes for light-harvesting antenna proteins LH-I and LH-II; reaction center polypeptides RC-L, RC-M, and RC-H; and enzymes of bacteriochlorophyll and carotenoid biosynthesis in Rhodobacter capsulatus by light and oxygen, Proc. Natl. Acad. Sci. USA 83: 7613–7617.

    Article  PubMed  CAS  Google Scholar 

  • Zucconi, A.P., and Beatty, J.T., 1988, Post-transcriptional regulation by light of the steady-state levels of mature B800–850 light-harvesting complexes in Rhodobacter capsulatus, J. Bacteriol. 170: 877–882.

    PubMed  CAS  Google Scholar 

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© 1990 Springer Science+Business Media New York

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Tadros, M.H. (1990). Gene Structure, Organisation and Expression of the Light-Harvesting B800-850 α and β Polypeptides in Photosynthetic Bacteria. In: Drews, G., Dawes, E.A. (eds) Molecular Biology of Membrane-Bound Complexes in Phototrophic Bacteria. FEMS Symposium. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-0893-6_4

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  • DOI: https://doi.org/10.1007/978-1-4757-0893-6_4

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4757-0895-0

  • Online ISBN: 978-1-4757-0893-6

  • eBook Packages: Springer Book Archive

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