The Electrogenic Event Associated with the Reduction of the Secondary Quinone Acceptor in Rhodobacter Sphaeroides Reaction Centers

  • A. Yu. Semenov
  • M. D. Mamedov
  • V. P. Shinkarev
  • M. I. Verkhovsky
  • N. I. Zakharova
Part of the FEMS Symposium book series (FEMSS)


An electrometric method was used to investigate flash-induced electrogenic stages in proteoliposomes containing photosynthetic RCs of Rhodobacter sphaeroides. Besides the very fast electrogenic step associated with primary dipole formation, an additional electrogenic stage with a rise-time of 0.25 ms (pH 7.5) appeared to be induced by even numbered flashes. The maximal amplitude of this stage contributes ~0.3 to the fast phase, associated with the charge separation between P870 and QA. The similarity of the rise-time of this phase, the time of the disproportioning reaction of semiquinones QA and QB and the rate of proton uptake by RCs, its appearance only after even-numbered flashes, the sensitivity to o-phenanthroline as well as the increase of its rise-time with pH indicate that the additional electrogenic phase arises from the reaction:
$$ {Q_A}^ - ({H^ + }){Q_B}^ - + {H^ + } - - \to {Q_A}{Q_B}{H_2} $$
A kinetic model is suggested describing the flash number dependence of the observed changes of the amplitude of this phase.


Quinone Acceptor Proton Binding Photoelectric Response Actinic Light Intensity Electrometric Method 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



reaction center


reaction center bacteriochlorophyll dimmer


primary and secondary quinine acceptors


transmembrane electric potential difference




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Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • A. Yu. Semenov
    • 1
  • M. D. Mamedov
    • 1
  • V. P. Shinkarev
    • 1
  • M. I. Verkhovsky
    • 1
  • N. I. Zakharova
    • 1
  1. 1.A.N. Belozersky Laboratory and Biological DepartmentMoscow State UniversityMoscowUSSR

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