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Spectroscopic and Structural Studies of Crystallized Reaction Centres from Wild Type Rhodobacter Sphaeroides Y

  • F. Reiss-Husson
  • B. Arnoux
  • A. Ducruix
  • K. Steck
  • W. Mäntele
  • M. Schiffer
  • C. H. Chang
Part of the FEMS Symposium book series (FEMSS)

Abstract

Spectroscopic studies on reaction centre (RC) crystals are a powerful tool to answer a number of questions. First one may ask if the pigments have been altered or not during the crystallization process. Second, photoinduced electron tranfer may be studied in the crystalline state and kinetics may be compared to those observed in solution. Finally, when the three-dimensionnal structure of the reaction center has been solved, the polarized absorption spectra may be analyzed to assign the individual absorption bands to oriented transition moments of each ohromophore [1,2].

Keywords

Reaction Centre Charge Recombination Linear Dichroism Secondary Quinone Measuring Light Intensity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. [1]
    Knapp, E. W., Fischer, S. F., Zinth, W., Sander, M., Kaiser, W., Deisenhofer, J. and Michel, H. (1985); Proc. Natl. Acad. Sci. USA 82, 8463–8467.PubMedCrossRefGoogle Scholar
  2. [2]
    Mäntele, W., Steck, K. Becker, A., Wacker, T., Welte, W., Gadon, N., and Drews, G. (1988); In: The Photosynthetic Bacterial Reaction Center: Structure and Dynamics (Breton, J. and Vermeglio, A. eds.) NATO ASI Series Vol. 149, pp. 33–39.CrossRefGoogle Scholar
  3. [3]
    Rutherford, A. W., Agalidis, I. and Reiss-Husson, F. (1985); FEBS Lett. 182, 151–157.CrossRefGoogle Scholar
  4. [4]
    Ducruix, A. and Reiss-Husson, F. (1987); J. Mol. Biol. 193, 419–421.PubMedCrossRefGoogle Scholar
  5. [5]
    Lutz, M., Agalidis, I., Hervo, G., Cogdell, R. and Reiss-Husson, F. (1978); Biochim. Biophys. Acta 503, 287–303.PubMedCrossRefGoogle Scholar
  6. [6]
    Lutz, M., Szponarski, W., Berger, G., Robert, B. and Neumann, J. M. (1987); Biochim. Biophys. Acta 894, 423–433.CrossRefGoogle Scholar
  7. [7]
    Reiss-Husson, F. and Mäntele, W. (1988); FEBS Lett. 239, 78–82CrossRefGoogle Scholar
  8. [8]
    Ducruix, A., Arnoux, B. and Reiss-Husson, F. (1988); in: The Photosynthetic Bacterial Reaction Center: Structure and Dynamics (Breton, J. and Vermeglio, A., eds.) NATO ASI Series, Vol. 149, 419–421Google Scholar
  9. [9]
    Agalidis, I. (1987); Eur. J. Biochem. 166, 235–239PubMedCrossRefGoogle Scholar
  10. [10]
    Chang, C. H., Tiede, D. M., Tang, J., Smith, U., Norris, J. and Schiffer, M. (1986); FEBS Lett. 205, 82–86PubMedCrossRefGoogle Scholar
  11. [11]
    Yeates, T. O., Komiya, H., Chirino, A., Rees, D. C., Allen, J. P. and Feher, G. (1988); Proc. Natl. Acad. Sci. USA 85, 7995–7997CrossRefGoogle Scholar
  12. [12]
    Taremi, S. S., Violette, C. A. and Frank, H.A. (1989); Biochim. Biophys. Acta 973, 86–92PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • F. Reiss-Husson
    • 1
  • B. Arnoux
    • 2
  • A. Ducruix
    • 2
  • K. Steck
    • 3
  • W. Mäntele
    • 3
  • M. Schiffer
    • 4
  • C. H. Chang
    • 4
  1. 1.UPR 407Gif sur YvetteFrance
  2. 2.ICSN, CNRSGif sur YvetteFrance
  3. 3.Institut für Biophysik und StrahlenbiologieFreiburgGermany
  4. 4.Biology DivisionArgonne National Lab.ArgonneUSA

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