Abstract
The primary photochemistry in bacterial photosynthesis involves a charge separation, stable for milliseconds to seconds, between specialized pigments acting as the primary electron donor and the intermediary electron acceptor, and quinones acting as primary and secondary electron acceptors. In bacterial reaction centers, the primary electron donor (P) is a bacteriochlorophyll (BChl) a or b dimer, the intermediary acceptor (H) is a bacteriopheophytin (BPheo) a or b monomer, and ubiquinones or menaquinones have been identified as electron acceptors (Q). The specifity, efficiency and stability of this charge separation relies on the arrangement and specific interaction of the pigments and redox components in the protein matrix. X-ray structures available for bacterial RC [1,2] provide a static picture of the quiescent state and suggest specific interactions of the pigments and quinones with their host site. However, additional information on the mechanisms and dynamics of primary electron transfer and on the concomitant change of interactions and protein conformation is required. Time-resolved optical spectroscopy (for a review, see [3]) as well as resonance Raman spectroscopy (for a review, see [4]) have provided such information.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
H. Michel, O. Epp and J. Deisenhofer; Pigment-Protein Interactions in the Photosynthetic Reaction Centre from Rhodopseudomonas Viridis; The EMBO Journal 5: 2445 (1986)
J.P. Allen, G. Feher, T.O. Yeates and D.C. Rees; Structure of the Reaction Center from Rhodobacter Sphaeroides R-26: The Cofactors; Proc. Natl. Acad. Sci. USA 84: 5730 (1988)
W.W. Parson; Photosynthetic Bacterial Reaction Centers; Ann. Rev. Biophys. Bioeng. 11:57 (1982)
M. Lutz; Resonance Raman Studies in Photosynthesis; In: Advances in Infrared and Raman Spectroscopy (Clark, R.H.J and , R.E., eds.) Wiley Heyden 11:211 (1984)
W. Mäntele, E. Nabedryk, B.A. Tavitian, W. Kreutz, and J. Breton; Light Induced Fourier Transform Infrared (FTIR) Spectroscopic Studies of the Primary Donor Oxidation in Bacterial Photosynthesis; FEBS Letters 187: 227 (1985)
E. Nabedryk, W. Mäntele, B.A. Tavitian and J. Breton; Light-Induced Fourier-Transform Infrared (FTIR) Spectroscopic Investigations of the Intermediary Acceptor Reduction in Bacterial Photosynthesis; Photochem. Photobiol. 43: 461 (1986)
W. Mäntele, A.M. Wollenweber, E. Nabedryk, J. Breton; Infrared Spectroelectrochemistry of Bacteriochlorophylls and Bacteriopheophytins: Implications for the Binding of the Pigments in the Reaction Center from Photosynthetic Bacteria; Proc. Natl. Acad. Sci. USA 85: 8468 (1988)
W. Mäntele, A.M. Wollenweber, F. Rashwan, J. Heinze, E. Nabedryk, G. Berger, J. Breton; Fourier-Transform Infrared Spectroelectrochemistry of the Bacteriochlorophyll Anion Radical; Photochem. Photobiol. 47: 451 (1988)
K. Ballschmiter, J.J. Katz; An Infrared Study of Chlorophyll-Chlorophyll and Chlorophyll-Water Interactions; J. Am. Chem. Soc. 91: 2661 (1969)
B.R. Clark and D.H. Evans; Infrared Studies of Quinone Radical Anions and Dianions Generated by Flow-Cell Electrolysis. J. Electroanal. Chem. 69: 181 (1976)
W. Mäntele, F. Siebert and W. Kreutz; Kinetic Properties of Rhodopsin and Bacteriorhodopsin Measured by Kinetic Infrared Spectroscopy (KIS) Methods in Enzymology 88: 729 (1982)
R. Hienerwadel, W. Kreutz and W. Mäntele; Time-Resolved Infrared Spectroscopy using Tunable Diode Lasers: Characterization of Intermediates in Light-Induced Electron Transfer of Photosynthesis; Procedings of the III European Conference on the Spectroscopy of Biological Molecules, Rimini (1989), in the press
M.S. Braiman and K.J. Rothschild; Fourier-Transform Infrared Techniques for Probing Membrane Protein Structure; Ann. Rev. Biophys. Biophys. Chem. 17: 541 (1988)
R. Hienerwadel, W. Kreutz and W. Mäntele; A Period-Four Infrared Signal from Active Water-Splitting Complex; Proceedings of the VIIIth International Congress on Photosynthesis; Stockholm 1989, in the press
G. Berger, A.M. Wollenweber, J. Kleo, S. Andrianambinintsoa, W. Mäntele; A Rapid Preparative Method for Purification of Bacteriochlorophyll A and B; J. Liq. Chrom. 10: 1519 (1987)
M. Leonhard, A.M. Wollenweber, G. Berger, J. Kleo, E. Nabedryk, J. Breton and W. Mäntele; Infrared spectroscopy and Electrochemistry of Chlorophylls: Model Compound Studies on the Interaction in their Native Environment; In: Techniques and New Developments in Photosynthesis; (Barber,J. ed.) in the press
J. Heinze; Cyclic Voltammetry — “Electrochemical Spectroscopy”; Angew. Chemie (Int. Ed. Engl.) 23:831 (1984)
M. Bauscher, K. Bagley, E. Nabedryk, J. Breton, W. Mäntele; Models for Ubiquinones and their Anions Involved in Photosynthetic Electron Transfer, Characterized by Thin-Layer Electrochemistry and FTIR/UV/VIS Spectroscopy; Proceedings of the VIIIth International Congress on Photosynthesis; Stockholm (1989), in the press
R.C. Prince, D.M. Tiede, J. P. Thornber and P.L. Dutton; Spectroscopic Properties of the Intermediary Electron Carrier in the Reaction Center of Rhodopseudomonas viridis; Biochim. Biophys. Acta 462: 467 (1977)
E. Nabedryk, S. Andrianambinintsoa, W. Mäntele, J. Breton; FTIR Investigations of the Intermediary Electron Acceptor Photoreduction in Purple Photosynthetic bacteria and Green Plants; In: The Photosynthetic Bacterial Reaction Center — Structure and Dynamics ( J. Breton & A. Vermeglio, eds) NATO ASI Series (1988), Plenum Press
M. Leonhard, E. Nabedryk, G. Berger, J. Breton, W. Mäntele; Model Compound Studies of Pigments Involved in Photosynthetic Energy Conversion: Infrared (IR) -Spectro-Electrochemistry of Chlorophylls and Pheophytins; Proceedings of the VIIIth International Congress on Photosynthesis,Stockholm (1989), in the press
B.A. Tavitian, E. Nabedryk, W. Mäntele, J. Breton; Light-Induced FourierTransfrom Infrared (FTIR) Spectroscopic investigations of Primary Reactions in Photosystem I and Photosystem II; FEES Lett 201: 151 (1986)
E. Nabedryk, S. Andrianambinintsoa, G. Berger, M. Leonhard, W. Mäntele, J. Breton; Characterization of Bonding Interactions of the Intermediary Electron Acceptor in the Reaction Center of Photosystem II by FTIR Spectroscopy; Manuscript submitted to BBA (July 1989)
K.A. Bagley, E. Abresch, M. Okamura, G. Feher, M. Bauscher, W. Mäntele, E. Nabedryk, J. Breton; FTIR studies of the D+Q −A and D+Q −B States in Reaction Centers from Rb. sphaeroides; Proceedings of the VIIIth International Congress on Photosynthesis; Stockholm (1989), in the press
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1990 Springer Science+Business Media New York
About this chapter
Cite this chapter
Mäntele, W., Leonhard, M., Bauscher, M., Nabedryk, E., Berger, G., Breton, J. (1990). The Binding and Interaction of Pigments and Quinones in Bacterial Reaction Centers Studied by Infrared Spectroscopy. In: Drews, G., Dawes, E.A. (eds) Molecular Biology of Membrane-Bound Complexes in Phototrophic Bacteria. FEMS Symposium. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-0893-6_37
Download citation
DOI: https://doi.org/10.1007/978-1-4757-0893-6_37
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4757-0895-0
Online ISBN: 978-1-4757-0893-6
eBook Packages: Springer Book Archive