Examination of the Rhodobacter Capsulatus Special Pair in Wild-Type and Heterodimer-Containing Reaction Centers by Time-Resolved Optically Detected Magnetic Resonance

  • Stephen V. Kolaczkowski
  • Edward J. Bylina
  • Douglas C. Youvan
  • James R. Norris
Part of the FEMS Symposium book series (FEMSS)


One of the exciting new techniques available to photosynthetic researchers is the ability to perform site-directed mutagenesis on the photosynthetic apparatus. The X-ray crystal structures of reaction centers (RCs) can be used to identify important amino acid residues (Michel et al., 1986; Chang et al., 1986; Yeates et al., 1988). Genetic manipulations that alter such residues allow for unprecedented experimental possibilities. Rb. capsulatus possesses the best-developed genetic system for the study of the photosynthetic apparatus (Scolnick and Marrs, 1987). A system of deletion strains (Youvan et al., 1985) and complementing plasmids (Bylina et al., 1986, 1989) has been used to generate a diverse collection of reaction center mutations. When the histidine at M200 was replaced with either leucine or phenylalanine, the (Bch1)2 primary donor is replaced with a Bchl-Bphe heterodimer (Bylina and Youvan, 1988). The isolation of heterodimer-containing RCs is one of the first true tests of the importance of a special pair type structure for efficient photosynthetic charge separation.


Reaction Center Radical Pair Deletion Strain Magnetic Field Effect Photosynthetic Reaction Center 


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Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • Stephen V. Kolaczkowski
    • 1
  • Edward J. Bylina
    • 2
  • Douglas C. Youvan
    • 2
  • James R. Norris
    • 1
  1. 1.Chemistry DivisionArgonne National LaboratoryArgonneUSA
  2. 2.Department of ChemistryMassachusetts Institute of TechnologyCambridgeUSA

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