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Carotenoid Absorbance Changes in Liposomes Reconstituted with Pigment-Protein Complexes from Rhodobacter Sphaeroides

  • Wim Crielaard
  • Klaas J. Hellingwerf
  • Wil N. Konings
Part of the FEMS Symposium book series (FEMSS)

Abstract

The electrochromic behaviour of carotenoids has been widely used to determine the electrical potential difference (∆ψ) across photosynthetic membranes, like chromatophores [1] and bacterial cells [2,3]. The carotenoid absorbance change has several advantages over other methods for recording the ∆ψ: (i) the method is non-invasive, (ii) the relationship between the ∆ψ and the absorbance change is linear and (iii) the method shows a rapid response time. The major disadvantage of the carotenoid band shift as a ∆ψ indicator is that a calibration of the ∆ψ dependent bandshifts is not (always) possible in every experimental system [3].

Keywords

Difference Spectrum Absorbance Change Photosynthetic Membrane Electrical Potential Difference Rhodopseudomonas Palustris 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • Wim Crielaard
    • 1
  • Klaas J. Hellingwerf
    • 1
  • Wil N. Konings
    • 1
  1. 1.Department of MicrobiologyUniversity of GroningenHarenThe Netherlands

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