Assembly of Intracytoplasmic Membranes in Rhodobacter Sphaeroides Mutants Lacking Light-Harvesting and Reaction Center Complexes
The intracytoplasmic membrane (ICM) of photosynthetic bacteria contains a number of integral bacteriochlorophyll (BCh1)-protein complexes. In Rhodobacter sphaeroides, these consist of photochemical reaction centers together with the B800-850 and B875 light-harvesting proteins1 which function as peripheral and core antennae, respectively. The B875 and reaction center complexes form the fixed cores of photosynthetic units and are found in a constant molar ratio of ~25:1, respectively . The B800-850 antenna comprises the variable portion of the photosynthetic unit and can reach levels more than three-fold greater than those of B875 under low illumination  or in the latter stages of induction of ICM formation at reduced oxygen tension . The ICM is continuous with the cytoplasmic membrane [4, 5] and in vivo surface labeling has demonstrated that the interior of the ICM is accessible from the periplasmic space [6, 7].
KeywordsRhodobacter Sphaeroides Photosynthetic Membrane Antenna Complex Reaction Center Complex Photosynthetic Unit
Unable to display preview. Download preview PDF.
- 1.R. J. Cogdell, H. Zuber, J. P. Thornber, G. Drews, G. Gingras, R. A. Niederman, W. W. Parson, and G. Feher, Recommendations for the naming of photochemical reaction centres and light-harvesting pigment-protein complexes from purple photosynthetic bacteria, Biochim. Biophys. Acta 806: 185 (1985).CrossRefGoogle Scholar
- 5.S. Kaplan and C. J. Arntzen, Photosynthetic membrane structure and function, in: “Photosynthesis,” Vol. 1, Govindjce, ed., p. 65, Academic Press, New York (1982).Google Scholar
- 7.G. S. Inamine, J. van Houten, and R. A. Niederman, Intracellular localization of photosynthetic membrane growth initiation sites in Rhodopseudomonas sphaeroides, J. Bacterial. 158: 425 (1984).Google Scholar
- 9.R. A. Niederman, C. N. Hunter, G. S. Inamine, and D. E. Mallon, Development of the bacterial photosynthetic apparatus, in: “Photosynthesis,Vol. 5, Chloroplast Development,” G. Akoyunoglou, ed., p. 663, Balaban, Philadelphia (1981).Google Scholar
- 11.J. R. Bowyer, C. N. Hunter, T. Ohnishi, and R. A. Niederman, Photosynthetic membrane development in Rhodopseudomonas sphaeroides: Spectral and kinetic characterization of redox components of light-driven electron flow in apparent photosynthetic membrane growth initiation sites, J. Biol. Chem. 260: 3295 (1985).PubMedGoogle Scholar
- 16.C. N. Hunter and R. van Grondelle, The use of mutants to investigate the organisation of the photosynthetic apparatus of Rhodobacter sphaeroides, in: “Photosynthetic Light-Harvesting Systems,” H. Scheer and P. Schneider, eds., p. 247, Walter de Gruyter, New York (1988).Google Scholar
- 20.C. N. Hunter, J. D. Pennoyer, J. N. Sturgis, D. Farrelly, and R. A. Niederman, Oligomerization states and associations of light-harvesting pigment-protein complexes of Rhodobacter sphaeroides as analyzed by lithium dodecyl sulfate-polyacrylamide gel electrophoresis, Biochemistry 27: 3459 (1988).CrossRefGoogle Scholar
- 21.J. N. Sturgis, C. N. Hunter, and R. A. Niederman, In preparation.Google Scholar
- 27.J. N. Sturgis and R. A. Niederman, Role of B800–850 light-harvesting pigment-protein complex in the morphogenesis of Rhodobacter sphaeroides membranes, in:“Proc. VIII Internat. Congr. Photosynth.”, In press.Google Scholar
- 28.W. H. J. Westerhuis, M. Vos, R. J. van Dorssen, R. van Grondelle, J. Amesz and R. A. Niederman, Associations of pigment-protein complexes in phospholipid-enriched bacterial photosynthetic membranes, in:“Biological Role of Plant Lipids,” P. A. Biacs; K. Gruiz, and T. Kremmer, eds., p. 227, Plenum, New York.Google Scholar