Advertisement

Rate-Limiting Endonucleolytic Cleavage of the 2.7 kb puf mRAN of Rhodobacter Capsulatus is Influenced by Oxygen

  • Gabriele Klug
  • Stanley N. Cohen
Part of the FEMS Symposium book series (FEMSS)

Abstract

Segmental differences in the stability of a polycistronic mRNA were first described for the R. capsulatus puf operon (Belasco et al., 1985). The proximal and distal segments of a 2.7 kb mRNA species encoding reaction center (RC) and light harvesting I (LHI) proteins are degraded at different rates, causing a tenfold molar excess of mRNA encoding LHI versus mRNA encoding RC proteins. The higher stability of the LHI-specific 0.5 kb mRNA segment results from protection against 3′ to 5′ exonucleases by an intercistronic mRNA region of secondary structure localized between the LHI genes (pufB, pufA) and the RC genes (pufL, pufM). Removal of this hairpin loop structure results in a change in the molar ratio of the 2.7 kb mRNA species and its 0.5 kb derivative and an altered stoichiometry of LHI and RC complexes in the membrane (Klug et al., 1.987).

Keywords

Reaction Center Phototrophic Bacterium Reaction Center Complex Segmental Difference Endonucleolytic Cleavage 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Adams, C.W., Forrest, M.E., Cohen, S.N., Beatty, T. (1989) J. Bacteriol. 171, 4 73 - 482.Google Scholar
  2. Bauer, C.E., Young, D.A., Marrs, B.L. (1988) J. Biol. Chem. 263, 4820-4827.Google Scholar
  3. Belasco, J. G., Beatty, J.T., Adams, C.W., von Gabain, A., Cohen, S.N. (1985) Cell 40, 171 - 181.PubMedCrossRefGoogle Scholar
  4. Chen, C.-Y. A., Beatty, J.T., Cohen, S.N., Belasco, J.G. (1988) Cell 52, 609-61 9.Google Scholar
  5. Clark, W.G., Davidson, E., Marrs, B.L. (1984) J. Bacteriol. 157, 945-948. Drews, G., Oelze, J. (1981) Adv. Microbiol. Physiol. 22, 1-97.Google Scholar

Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • Gabriele Klug
    • 1
  • Stanley N. Cohen
    • 2
  1. 1.Zentrum fur Molekulare BiologieHeidelbergGermany
  2. 2.Department of GeneticsStanford University, School of MedicineStanfordUSA

Personalised recommendations