Formation and Assembly of Alphavirus Glycoproteins
Among the variety of enveloped viruses that exist in nature, the Togaviridae family is generally considered to be among the simplest with regard to structure and composition of the virus. The virion structure is examined in detail in Chapter 22. In this chapter, we focus on the major protein components of the virion envelope and describe modifications of these proteins that occur as they mature from nascent polypeptides to fully mature and functional glycoproteins that form the spikes of the infectious particle. Most of our information about togavirus glycoproteins comes from biochemical studies of the two alphaviruses, Semliki Forest virus and Sindbis virus (Garoff et al., 1982). These viruses have provided valuable models for the analysis of membrane proteins.
KeywordsChinese Hamster Ovary Cell Glycosylation Site Vesicular Stomatitis Virus Glucose Residue Virus Glycoprotein
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