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Hapten — Sandwich Labeling of Cell-Surface Antigens

  • Leon Wofsy
  • Claudia Henry
  • Salvatore Cammisuli
Part of the Contemporary Topics in Molecular Immunology book series (CTI)

Abstract

The indirect antibody technique for amplified detection of antigens (Coons, 1956) has a wide and expanding range of applications. In general, this well-established method employs second-layer antiimmunoglobulin, coupled to an appropriate detector, to label first-layer antibody specific for a particular antigen. The last few years have seen the introduction of hapten-sandwich labeling procedures (Lamm et al,1972; Wofsy et al.,1974; Cammisuli and Wofsy, 1976) that circumvent the requirement of the conventional sandwich technique for antibodies from one species directed against the immunoglobulins of another. Hapten-sandwich labeling uses instead detector-coupled antihapten antibodies to amplify first-layer antibodies to which the hapten has been covalently attached.

Keywords

Hapten Group Biotin Hydrazide Avidin Molecule Conventional Sandwich Trinitrobenzene Sulfonate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations used in this chapter

ars

P-azobenzene

benz

p-azobenzoate

BzPen

benzylpenicilloyl

Con A

concanavalin A

DHB

methyl 3,5-dihydroxybenzimidate

DNP

2,4-dinitrophenyl

FACS

fluorescence-activated cell sorter

FcR

Fc receptors

FI

fluorescein

gal

p-azophenyl ß-D galactoside

glut

benzoyl-L-glutamic acid

HB

methyl p-hydroxybenzimidate

KLH

keyhole limpet hemocyanin

lac

p-azophenyl ß D lactoside

NIP

4-hydroxy-3-iodo-5-nitrophenylacetyl

PBS

0.01 M phosphate buffer containing 8.5 mg/m1 NaCI

PFC

plaque-forming cells

Rh

rhodamine

SRBC

sheep red blood cells

sulf

p-azobenzene sulfonate

TMA

p-azotrimethylanilinium

TNP

2,4, 6-tinitrophenyl

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Copyright information

© Springer Science+Business Media New York 1978

Authors and Affiliations

  • Leon Wofsy
    • 1
  • Claudia Henry
    • 1
  • Salvatore Cammisuli
    • 2
  1. 1.Department of Bacteriology and ImmunologyUniversity of CaliforniaBerkeleyUSA
  2. 2.Basel Institute for ImmunologyBaselSwitzerland

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