Advertisement

H-2 Antigens pp 815-825 | Cite as

Fc Receptor-Ligand Interaction Induces Activation of Human Natural Killer Cells

  • Ignacio Anegon
  • Maria Cristina Cuturi
  • Giorgio Trinchieri
  • Bice Perussia
Part of the NATO ASI Series book series (NSSA, volume 144)

Abstract

Interaction of NK cells with Sepharose-linked anti-Fc receptor (FcR, CD16 antigen) antibodies inhibits their ability to mediate both spontaneous and antibody-dependent cytotoxicity, whereas bivalent anti-CD16 antibodies enhance spontaneous cytotoxicity. Treatment of cultured NK cells with Sepharose-linked anti-CD16 antibodies induces expression of IL-2 and transferrin (Tf) receptors and of 4F2 antigens and this effect is enhanced in the presence of IL-2 during treatment. Analogously, treatment of NK cells with the anti-CD16 antibodies or immune complexes synergizes with IL-2 in inducing production of IFNγ and TNF. The combined treatment of NK cells with IL-2 and anti-CD16 antibodies induces cytoplasmic accumulation of mRNA transcripts for IL-2R and for the two lymphokines. Nuclear run-on experiments show that gene transcription is induced by the two stimuli. These data indicate that FcR are specialized molecules on NK cell surface that mediate signal transduction upon ligand binding. These effects may be similar to those induced in NK cells upon target cell recognition.

Keywords

Large Granular Lymphocyte Human Natural Killer Cell Daudi Cell Peripheral Blood Mononuclear Cell Culture Peripheral Blood Natural Killer Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Cuturi, M. C., Murphy, M., Costa-Giomi, M. P., Weinmann, R., Perussia, B., and Trinchieri, G.: Independent regulation of tumor necrosis factor and lymphotoxin production by human peripheral blood lymphocytes. J. Exp. Med. 165: 1581–1594, 1987PubMedCrossRefGoogle Scholar
  2. Degliantoni, G., Murphy, M., Kobayashi, M., Francis, M-K., Perussia, B., and Trinchieri, G.: Natural Killer (NK) cell-derived hematopoietic colony-inhibiting activity and NK cytotoxic factor. Relationship with tumor necrosis factor and synergism with immune interferon. J. Exp. Med. 162: 1512–1530, 1985PubMedCrossRefGoogle Scholar
  3. Gariglio, P., Bellard, M., and Chambon, P.: Clustering of RNA polymerase B molecules in the 5’ moiety of the adult g-globin gene of ben erythrocytes. Nucl. Acids Res. 9: 2589–2598, 1981PubMedCrossRefPubMedCentralGoogle Scholar
  4. Groudine, M., Peretz, M., and Weintraub, H.: Transcriptional regulation of hemoglobin switching in chicken embryos. Mol. Cell. Biol. 1: 281–288, 1981PubMedCentralPubMedGoogle Scholar
  5. Isakov, N., Scholz, W., and Altman, A.: Signal transduction and intracellular events in T lymphocyte activation. Immunol. Today 7: 271–276, 1986CrossRefGoogle Scholar
  6. Krönke, M., Leonard, W. J., Depper, J. M., Arya, S. K., Woong-Staal, F., Gallo, R. C., Waldman, T. A., and Greene, W. C.: Cyclosporin A inhibits T-cell growth factor gene expression at the level of mRNA transcription. Proc. Natl. Acad. Sci. USA 81: 5214–5218, 1984PubMedCrossRefPubMedCentralGoogle Scholar
  7. Lanier, L. L., Cwirla, S., Federspiel, N., and Phillips, J. H.: Human natural killer cells isolated from peripheral blood do not rearrange T cell antigen receptor chain genes. J. Exp. Med. 163: 209–214, 1986PubMedCrossRefGoogle Scholar
  8. Liu, C-C., Perussia, B., Cohn, Z. A., and Young, J. D-E.: Identification of a pore-forming protein of human peripheral blood NK cells. J. Exp. Med. 164: 2061–2076, 1986PubMedCrossRefGoogle Scholar
  9. Malavasi, F., Tetta, C., Funaro, A., Bellone, G., Ferrero, E., Collifranzone, A., Della Bona, P., Rusci, R., Matera, L., Camussi, G., and Caligaris Cappio, F.: Fc receptor triggering induces expression of surface-activation antigens and release of platelet-activating factor in large granular lymphocytes. Proc. Natl. Acad. Sci. (USA) 83: 2443–2447, 1986CrossRefGoogle Scholar
  10. Maniatis, T., Fritsch, E. F., and Sambrook, J.: Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory. N. Y. 1982Google Scholar
  11. Metzger, J., and Ishizaka, T.: Transmembrane signaling by receptor aggregation: the mast cell receptor for IgE as a case study. Fed. Proc. Am. Soc. Exp. Biol. 41: 7–34, 1982Google Scholar
  12. Murphy, M., Loudon, R., Kobayashi, M., and Trinchieri, G.: Gamma interferon and lymphotoxin, released by activated T cells, synergize to inhibit granulocyte-monocyte colony formation. J. Exp. Med. 164: 263–279, 1986PubMedCrossRefGoogle Scholar
  13. Perussia, B., Acuto, O., Terhorst, C., Faust, J., Lazarus, R., Fanning, V., and Trinchieri, G.: Human natural killer cells analyzed by B73.1, a monoclonal antibody blocking FcR functions. II. Studies of B73.1 antibody-antigen interaction on the lymphocyte membrane. J. Immunol. 130: 2142–2148, 1983aPubMedGoogle Scholar
  14. Perussia, B., Ramoni, C., Anegon, I., Cuturi, M. C., Faust, J., and Trinchieri, G.: Preferential proliferation of natural killer cells among peripheral blood mononuclear cells cocultured with B lymphoblastoid cell lines. Submitted for publication 1987Google Scholar
  15. Perussia, B., Starr, S., Abraham, S., Fanning, V., and Trinchieri, G.: Human natural killer cells analyzed by B73.1, a monoclonal antibody blocking Fc receptor functions. I. Characterization of the lymphocyte subset reactive with B73.1. J. Immunol. 130: 2133–2141, 1983bPubMedGoogle Scholar
  16. Perussia, B., and Trinchieri, G.: Inactivation of natural killer cell cytotoxic activity after interaction with target cells. J. Immunol. 126: 754–758, 1981PubMedGoogle Scholar
  17. Perussia, B., and Trinchieri, G.: Antibody 3G8, specific for the human neutrophil Fc receptor, reacts with natural killer cells. J. Immunol. 132: 1410–1415, 1984PubMedGoogle Scholar
  18. Perussia, B., Trinchieri, G., and Cerottini, J-C.: Functional studies of Fc receptor-bearing human lymphocytes: effect of treatment with proteolytic enzymes. J. Immunol. 123: 681–687, 1979PubMedGoogle Scholar
  19. Perussia, B., Trinchieri, G., Jackson, A., Warner, N. L., Faust, J., Rumpold, H., and Lanier, L. L.: The Fc receptor for IgG on human natural killer cells: phenotypic, functional and comparative studies using monoclonal antibodies. J. Immunol. 133: 180–189, 1984PubMedGoogle Scholar
  20. Reynolds, C. W., Bonyhadi, M., Herberman, R. B., Young, H. A., and Hedrick, S. M.: Lack of gene rearrangement and mRNA expression of the beta chain of the T cell receptor in spontaneous rat large granular lymphocyte leukemia lines. J. Exp. Med. 161: 1249–1254, 1985PubMedCrossRefGoogle Scholar
  21. Trinchieri, G., and Perussia, B.: Human natural killer cells: biologic and pathologic aspects. Lab. Invest. 50: 489–513, 1984PubMedGoogle Scholar
  22. Tutt, M. M., Kuziel, W. A., Hackett, J. J., Bennett, M., Tucker, P. W., and Kumar, V.: Murine natural killer cells do not express functional transcript of the a, B, or y chain genes of the T cell receptor. J. Immunol. 137: 2998–3001, 1986PubMedGoogle Scholar
  23. Van De Griend, R. J., Bolhuis, R. L. H., Stoter, G., and RooTemond, R. C.: Regulation of cytolytic activity in CD3 and CD3 killer cell clones by monoclonal antibodies (anti-CD16, anti-CD2, anti-CD3) depends on subclass specificity of target cell IgG-FcR. J. Immunol. 138: 3137–3144, 1987PubMedGoogle Scholar
  24. Wright, S. C., and Bonavida, B.: Studies on the mechanism of natural killer cytotoxicity. II. Coculture of human PBL with NK-sensitive or resistant cell lines stimulates release of natural killer cytotoxic factors (NKCF) selectively cytotoxic to NK-sensitive target cells. J. Immunol. 130: 2479–2483, 1983PubMedGoogle Scholar
  25. Wright, S. C., and Bonavida, B.: Studies on the mechanism of natural killer cell-mediated cytotoxicity. VII. Functional comparison of human natural killer cytotoxic factors with recombinant lymphotoxin and tumor necrosis factor. J.Immunol. 138: 1791–1798, 1987PubMedGoogle Scholar
  26. Zarcone, D., Prasthofer, E. F., Malavasi, F., Pistoia, V., LoBuglio, A. F., and Grossi, C.: Ultrastructural analysis of human natural killer cell activation. Blood 69: 1725–1736, 1987PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1987

Authors and Affiliations

  • Ignacio Anegon
    • 1
  • Maria Cristina Cuturi
    • 1
  • Giorgio Trinchieri
    • 1
  • Bice Perussia
    • 1
  1. 1.The Wistar Institute of Anatomy BiologyPhiladelphiaUSA

Personalised recommendations