H-2 Antigens pp 801-814 | Cite as

Use of Congenic and Recombinant Inbred Mouse Strains to Link a Highly Conserved Idiotype Marker to an Igh-C Region Allotype Locus and to Map This New Member of the Q52 Gene Family Within the Igh-V Gene Complex

  • Sheng-Chang Luo
  • Richard B. Bankert
Part of the NATO ASI Series book series (NSSA, volume 144)


The gene coding for a highly conserved idiotype marker associated with the murine response to the hapten phthalate (Xmp) was linked to the IgCH allotype locus using a pair of congenic resistant mouse strains, CB.20 and BC.8. These two strains of mice produce the same level of anti-phthalate antibody when immunized with phthalate-KLH. However, the idiotype marker identified as CRI-Xmp was found in the anti-phthalate antibody obtained from BC.8 but not CB.20 immune mice. The presence or absence of the CRI-Xmp in the phthalate immune sera of several different wild mouse populations was also monitored. In these studies, we observed some nonconcordance in the expression of the idiotype and the heavy chain allotype marker. This nonconcordance is most likely due to a crossover event occuring between Igh-C and Igh-V gene complexes. Using BXD recombinant inbred strains, the VH-Xmp was mapped with respect to other known VH gene markers. Based upon these studies VH-Xmp was found to map close to the constant region immunoglobulin gene cluster, i.e., between VH-S14 and VH-Ox. Preliminary sequence data indicate the VH-Xmp is a member of the Q52 gene family.


Inbred Strain Immune Seron Antiidiotypic Antibody Congenic Mouse Strain Immunoglobulin Heavy Chain Variable Region 


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  1. Baily, D.W., 1971, Recombinant inbred strains. Transplantation 11: 325–327.CrossRefGoogle Scholar
  2. Bankert, R.B., Mazzaferro, P.K. and Mayers, G.L.,1981, Hybridomas producing hemolytic plaques used to study the relationship between monoclonal antibody affinity and the efficiency of plaque inhibition with increasing concentrations of antigen. Hybridoma 1: 47–58.Google Scholar
  3. Bloor, A.G., Jou, Y-H., Hoeplinger, C., Gartner, J.E., Mayers, G.L. and Bankert, R.B., 1982, Hapten-specific B cell repertoire probed by hybridoma technology: Selection and characterization of representative clonotypes from the antibody-forming cell pool. J. Immunol. 128: 1443–1449.PubMedGoogle Scholar
  4. Brodeur, P.H. and Riblet, R., 1984, The immunoglobulin heavy chain variable region (Igh-V) locus in the mouse. I. One hundred Igh-V genes comprise seven families of homologous genes. Eur. J. Immunol. 14: 922–930.PubMedCrossRefGoogle Scholar
  5. Edelman, G.M. and Gottlieb, P.D., 1970, A genetic marker in the variable region of light chains of mouse immunoglobulins. Proc. Natl. Acad. Sci, USA 67: 1192–1199.PubMedCrossRefGoogle Scholar
  6. Hart, D.A., Wang, A.L., Pawlak, L.L. and Nisonoff, A., 1972, Suppression of idiotypic specificities in adult mice by administration of antiidiotypic antibodies. J. Exp. Med. 135: 1293–1300.PubMedCentralPubMedCrossRefGoogle Scholar
  7. Jou, Y-H., Luo, S-C. and Bankert, R.B., 1983, A filtration double antibody radioimmunoassay that simplifies and semi-automates the isolation of immune precipitates. J. Immunol. Methods 65: 285–292.PubMedCrossRefGoogle Scholar
  8. Kaartinen, M., Griffiths, G., Markham, A.F., and Milstein, C., 1983, mRNA sequences define an unusually restricted IgG response to 2-phenyloxazolone and its early diversification. Nature 304: 320–324.Google Scholar
  9. Kipps, T.J. and Dorf, M.E., 1979, Genetic mapping of the BGL idiotypic marker within the Igh-V region. Immunogenetics 9: 297–302.CrossRefGoogle Scholar
  10. Lieberman, R., Potter, M., Humphrey, W., Jr. and Chien, C.C., 1976, Idiotypes of insulin-binding antibodies and myeloma proteins controlled by genes linked to the allotype locus of the mouse. J. Immunol. 117: 2105–2111.PubMedGoogle Scholar
  11. Luo, S.C. and Bankert, R.B., 1985, Cross-reactive idiotype family observed in the phthalate-specific B cell repertoire of adult BALB/c mice: Diversity of IgM compared with IgG monoclonal anti-phthalate antibodies. J. Immunol. 135: 1252–1258.PubMedGoogle Scholar
  12. Luo, S.C. and Bankert, R.B., 1987, A new cross-reactive idiotype-defined family in the phthalate humoral immune response of mice. I. Linkage of VH-Xmp to IgCH allotype locus and mapping with respect to other known VH genes. J. Immunol. 138: 2311–2315.PubMedGoogle Scholar
  13. Luo, S-C. and Bankert, R.B., 1985, Characterization of a new cross-reactive idiotype family and its manipulation in vivo by the administration of anti-idiotype antibodies. Fed. Proc. (Abst.) 44: 1691.Google Scholar
  14. Luo, S-C. and Bankert, R.B., 1985, Monoclonal antibodies specific for the mouse IgG1 allotypic determinants: Reactivity with inbred and outbred mice. Hybridoma 4: 319–329.PubMedCrossRefGoogle Scholar
  15. Makela, O., Karjalainen, K. and Potter, M., 1979, Evolutionary conservation of a gene which controls the VH regions of an anti-hapten antibody (Np). Ann. Immunol. (Inst. Pasteur) 130C: 215–223.Google Scholar
  16. Makela, O., Seppala, I.J.T., Pelkonen, J., Kaartinen, M., Cazenave, P.-A. and Gefter, M.L., 1984, Crossing-over frequency in the Igh region of the mouse genome. Ann. Immunol. (Inst. Pasteur) 135C: 169–173.CrossRefGoogle Scholar
  17. Mayers, G.L. and Bankert, R.B., 1980, Immunochemistry of monoclonal antibodies. Transpl. Proc. 12: 413–416.Google Scholar
  18. Mayers, G.L., Grossberg, A. and Pressman, D., 1973, Arginine and lysine in binding sites of anti-4-azophthalate antibodies. Immunochemistry 10: 37–41.PubMedCrossRefGoogle Scholar
  19. Perlmutter, R.M., Kearney, J.F., Chang, S.P. and Hood, L.E., 1985, Developmentally controlled expression of immunoglobulin VH genes. Science 227: 1597–1600.PubMedCrossRefGoogle Scholar
  20. Snell, G.D., 1948, Methods for the study of histocompatibility genes. J. Genetics 49: 87–108.CrossRefGoogle Scholar
  21. Yancopoulos, G.D., Desiderio, S., Paskind, M., Kearney, J.F., Baltimore, D. and Alt, F., 1984, Preferential utilization of the most D-proximal VH gene segments in pre-B cell lines. Nature 311: 727–733.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1987

Authors and Affiliations

  • Sheng-Chang Luo
    • 1
  • Richard B. Bankert
    • 1
  1. 1.Department of Molecular ImmunologyRoswell Park Memorial InstituteBuffaloUSA

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