H-2 Antigens pp 719-724 | Cite as

H-2-linked Control of the Susceptibility of Mice to Cleft Palate Induced by Cortisone and Testosterone

  • David L. Gasser
  • Chhanda Gupta
  • Allen S. Goldman
Part of the NATO ASI Series book series (NSSA, volume 144)


In the course of mapping H–2–linked genes which control susceptibility to cortisoneinduced cleft palate, we have observed that certain pairs of congenic strains which have been considered to have the same H–2–recombinant chromosomes differ in their susceptibility. The B10.A(1R) and B10.A(2R) congenic strains have been believed to possess the same a/b crossover in the S/D interval, but B10.A(2R) is significantly more susceptible than B10.A(1R). Likewise, the B10.A(18R) and B10.BAR5 strains have been considered to have the same b/a crossover in the S/D interval, but B10.BAR5 is significantly more susceptible than B10.A(18R). In order to assess the possible effects of these genes on susceptibility to other steroids, we have injected pregnant mice on days 11 through 14 with testosterone. We have observed significant differences among H-2 congenic strains in the frequency of testosterone-induced fetal resorption. Among surviving fetuses, there were significant differences in the frequency of testosterone-induced cleft palate.


Glucocorticoid Receptor Cleft Palate Mouse Mammary Tumor Virus Pregnant Mouse Congenic Strain 


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  1. Biddle, F.G. And Fraser, F.C.: Cortisone-induced cleft palate in the mouse. A search for the genetic control of the embryonic response trait. Genetics 85: 289–302, 1977PubMedPubMedCentralGoogle Scholar
  2. Bonner, J.J. and Slavkin, H.C.: Cleft palate susceptibility linked to histocompatibility-2 (H-2) in the mouse. Immunogenetics 2: 213–218, 1975Google Scholar
  3. Bonner, J.J. and Tyan, M.L.: Glucocorticoid-induced cleft palate genes in chromosome 17: Genetic linkage and mapping analyses. Immunogentics 20: 169–183, 1984Google Scholar
  4. Cato, A.C.B., Henderson, D. and Ponta, H.: The hormone response element of the mouse mammary tumor virus DNA mediates the progestin and androgen induction of transcription in the proviral long terminal repeat region. EMBO Journal 6: 363 — 368, 1987PubMedPubMedCentralGoogle Scholar
  5. Darbre, P., Page, M. and King, R.J.B.: Androgen regulation by the long terminal repeat of mouse mammary tumor virus. Mol. Cell Biol. 6: 2847 — 2854, 1986PubMedCentralPubMedGoogle Scholar
  6. Demant, P.: Corticosteroid-induced cleft palate: Cis interaction of MHC genes and hybrid resistance. Immunogenetics 22: 183–188, 1985Google Scholar
  7. Dlouhy, S.R., Taylor, B.A. and Karn, R.C.: The genes for mouse salivary androgen-binding protein (ABP) subunits alpha and gamma are located on chromsome 7. Genetics 115: 535 — 543, 1987Google Scholar
  8. Francke, V. and Gehring, U.: Chromosome assignment of a murine glucocorticoid receptor gene (Grl-1) using intraspecies somatic cell hybrids. Cell 22: 657 — 664, 1980PubMedCrossRefGoogle Scholar
  9. Gasser, D.L. and Goldman, A.S.: Genetic and biochemical studies on glucocorticoid-induced cleft palate. Biochemical Actions of Hormones 10: 357 — 382, 1983Google Scholar
  10. Gasser, D.L., Mele, L., Lees, D.D. and Goldman, A.S.: Genes in mice that affect susceptibility to cortisone-induced cleft palate are closely linked to Ir genes on chromsomes 2 and 17. Proc. Natl. Acad. Sci. USA 78: 3147–3150, 1981Google Scholar
  11. Goldman, A.S. and Katsumata, M.: Murine glucocorticoid receptors: New evidence for a discrete receptor influenced by H-2. Arch. Biochem. Biophys. 249: 316–325, 1986Google Scholar
  12. Gregorova, S. and Ivanyi, P.: H-2-Associated genetic differences in androgen-dependent traits. The effect of testosterone injections. Foila biologica 19: 337–345, 1973Google Scholar
  13. Haseman, J.K. and Hogan, M.D.: Selection of the experimental unit in teratology studies. Teratology 12: 165–172, 1975Google Scholar
  14. Ivanyi, P., Gregorova, S. and Mickova, M.: Genetic differences in thymus, lymph node, testes and vesicular gland weight among inbred mouse strains. Association with the major histocompatibility (H-2) system. Foila biologica 18: 81–97, 1972aGoogle Scholar
  15. Ivanyi, P., Hampl, R., Starka, L. and Mickova, M.: Genetic association between H-2 gene and testosterone metabolism in mice. Nature New Biology 238: 280–281, 1972bGoogle Scholar
  16. Klein, J., Flaherty, L., VandeBerg, J.L. and Shreffler, D.C.: H-2 haplotypes, genes, regions and antigens: First listing. Immunogenetics 6: 489–512, 1978Google Scholar
  17. Lafuse, W. and Edidin, M.: Influence of the major histocompatibility complex, H-2, on liver adenylate cyclase activity and on glucagon binding to liver cell membranes. Biochemistry 19: 49–54, 1980PubMedCrossRefGoogle Scholar
  18. Litwack, G. and Rosenfield, S.A.: Liver cytosol corticosteroid binder IB, a new binding protein. J Biol. Chem. 250: 6799–6805, 1975Google Scholar
  19. Meruelo, D. and Edidin, M.: The biological function of the major histocompatibility complex: Hypotheses. Contemp. Topics Immunobiol 9: 231–253, 1980CrossRefGoogle Scholar
  20. Tyan, M.L. and Miller, K.K.: Genetic and environmental factors in cortisone-induced cleft palate. Proc. Soc. Exp. Biol. Med. 158: 618–621, 1978Google Scholar
  21. Warner, C.M., Gollnick, S.O. and Goldbard, S.B.: Linkage of the Preimplantation-Embryo-Development (Ped) gene to the mouse major histocompatibility complex (MHC). Biol. Reprod. 36: 606–610, 1987Google Scholar

Copyright information

© Springer Science+Business Media New York 1987

Authors and Affiliations

  • David L. Gasser
    • 1
  • Chhanda Gupta
    • 1
  • Allen S. Goldman
    • 2
  1. 1.Department of Human GeneticsUniversity of Pennsylvania School of Medicine, and Children’s Hospital of PhiladelphiaPhiladelphiaUSA
  2. 2.Center for Craniofacial Anomalies and Department of PediatricsThe University of Illinois College of Medicine at ChicagoUSA

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