The Origin of Lyt-2+ Class II Specific Allogeneic CTL
T lymphocytes recognize antigens in the context of MHC molecules and can be divided into two major subpopulations according to the expression of the two types of murine accessory molecules, Lyt-2 and L3T4 antigens. A general correlation has been noted between the class of MHC antigens recognized by T cells and the type of accessory molecules they express, with Lyt-2+ lymphocytes recognizing antigens in the context of class I molecules and L3T4+ lymphocytes in the context of class II molecules (1,2). However, CTLs specific for class II alloantigens do not follow this rule. In fact, allogeneic CTLs induced against disparities at class II loci were found to consist of comparable numbers of Lyt-2+ and L3T4+ cells (3,4). A part of such Lyt-2+ exceptions could be explained by the existence of clones recognizing allogeneic class II antigens in the context of class I antigens as exemplified by several isolated CTL clones (5). Nevertheless, there still remains a major population of Lyt-2+ cells with genuine class II specificity, as judged from four lines of evidence; genetic mapping, sensitivity to the blocking effect of monoclonal antibodies, failure to recognize I-A mutant cells, and ability to lyse cells transfected with alpha and beta genes of the I-A antigen (6). This population of CTL turned out to be extremely insensitive to the blocking effect of antibodies to the Lyt-2 antigen (3,4,6). Thus, such cells with “wrong phenotype” may have been triggered by class II antigens because of their independence from function of the accessory molecule.
KeywordsSpleen Cell Precursor Pool Accessory Molecule Genuine Class Radiation Bone Marrow Chimera
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- 2.D.P. Dialynas, D.B. Wilde, P. Marrack, A. Pierres, K.A. Wall, W. Havran, G. Otten, M.R. Loken, M. Pierres, J. Kappler, and F.W. Fitch. Characterization of the murine antigenic determinant, designated L3T4a recognized by monoclonal antibody GK1.5: Expression of L3T4a by functional T cell clones appears to correlate primarily with class II MHC antigen reactivity. Immunol. Rev. 74:29 (1983).PubMedCrossRefGoogle Scholar
- 4.N. Shinohara. Class II antigen-specific murine cytolytic T lymphocytes (CTL). I. analysis of bulk populations and establishment of Lyt-2+1,3T4- and Lyt-2-L3T4+ bulk CTL lines. Cell. Immunol. in press (1987).Google Scholar
- 6.N. Shinohara, N. Hozumi, M. Watanabe, J.A. Bluestone, R. Johnson-Leva, and D.H. Sachs. Class II antigen specific murine cytolytic T lymphocytes (CTL). II. Genuine class II specificity of Lyt-2-r CTL clones. submitted (1987).Google Scholar
- 8.H. Auchincloss, Jr., K. Ozato and D.H. Sachs. Two distinct murine differentiation antigens determined by genes linked to the Ly-6 locus. J. Immunol. 127:1839 (1981).Google Scholar
- 9.F.J. Dumont, L.Z. Coker, R.C. Habbersett and J.A. Treffinger. Xenogeneic monoclonal antibody to an Ly-6-linked murine cell surface antigen: Differential reactivity with T cell subpopulations and bone marrow cells. J. Immunol. 134:2357 (1985).Google Scholar