Abstract
We examined the ability of peptides corresponding to different regions of hen eggwhite lysozyme to stimulate proliferative T-cell responses in several mouse strains. The relationship of the major histocompatibility complex (MHC) and other non-MHC genes to the T cell repertoire was studied. It was found that the MHC was the major influence on the specificity of the T cell repertoire. The T cell response following immunization with native hen eggwhite lysozyme was also investigated. Challenge with the whole protein preferentially induces responses to some determinants compared to others. Hence, some regions of the protein appear to be immunodominant while other determinants are sub-dominant.
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Allen, P.M., McKean, D.J., Beck, B.N., Sheffield, J. and Glimcher, L.H. (1985), Direct evidence that a class II molecule and a simple globular protein generate multiple determinants. J Exp Med 162: 1264.
Benjamin, C.D., Berzofsky, J.A., East, I.J., Gurd, F.R.N., Hannum, C., Leach, S.J, Margoliash, E., Michael, J.G., Miller, A., Prager E.M. Reichlin, M., Sercarz, E.E., Smith-Gill, S.J., Todd, P.E. and Wilson, A.C., (1984), The antigenic structure of proteins: A reappraisal. Ann. Rev. Immunol. 2: 67–101.
Berkower, I., Matis, L.A., Buckenmeyer, G.K., Gurd, F.R.N., Longo, D.L. and Berzofsky, J.A., (1984), Identification of distinct predominant epitopes recognized by myoglobin-specific T-cells under the control of different Ir genes and characterization of representative T-cell clones. J. Immunol. 132: 1370.
Bixler, G.S., Yoshida, T. and Atassi, M.Z., (1985), Antigen presentation of lysozyme: T cell recognition of peptide and intact protein after priming with synthetic overlapping peptides comprising the entire protein chain. Immunology 56: 103.
Finnegan, A., Smith, M.A., Smith, J.A., Berzofsky, J.A., Sachs, D.M. and Hodes, R.J., (1986), The T cell repertoire for recognition of a phylogenetically distant protein antigen. Peptide specificity and MHC restriction of Staphylococcal nuclease-specific T cell clones. J. Exp. Med. 164: 897.
Gammon, G., Shastri, N., Cogswell, J., Wilbur, S., Sadegh-Nasseri, S., Krzych, U., Miller, A., and Sercarz, E., (1987), The choice of T cell epitopes utilized on a protein antigen depends on factors distant from, as well as at, the determinant site. Immunol. Reviews. 98, In press.
Katz, M., Maizels, R., Wicker, L., Miller, A., and Sercarz, E.E., (1982), Immunological focussing by the mouse MHC: mouse strains confronted with distantly related lysozymes confine their attention to very few epitopes. Eur. J. Immunol. 12: 535–540.
Livingstone, A.M. and Fathman, C.G., (1987), The structure of T-cell epitopes. Ann. Rev. Immunol. 5: 477.
Maizels, R., Clarke, J., Harvey, M., Miller, A. and Sercarz, E.E., (1980), Epitope specificity of the T cell proliferative response to lysozyme: T proliferative cells react predominantly to different determinants from those recognized by B cells. Eur. J. Immunol. 10: 509–515.
Margalit, M., Spouge, J., Cornette, J., Cease, K., DeLisi, C. and Berzofsky, J.A., (1987), Prediction of immunodominant helper T cell antigenic sites from the primary sequence. J. Immunol. 138: 2213.
Rothbard, J., Townsend, A., Edwards, M., and Taylor W., (1986), Pattern recognition among T cell epitopes, in: “Modern Trends in Human Leukemia VII”.
Shastri, N., Gammon, G., Miller, A. and Sercarz, E.E., (1986), la molecule associated selectivity in T-cell recognition of a 23 amino acid peptide of lysozyme. J. Exp. Med. 164: 882–896.
Solinger, A.M., Ultee, M.E., Margoliash, E. and Schwartz, R.H., (1979), Lymphocyte response to cytochrome c. Demonstration of a T-cell heteroclitic proliferative response and identification of a topographic antigenic determinant on pigeon cytochrome c whose immune recognition requires two complementary immune response genes. J. Exp. Med. 150: 830.
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Gammon, G. et al. (1987). The Major Histocompatibility Complex and the T Cell Repertoire. In: David, C.S. (eds) H-2 Antigens. NATO ASI Series, vol 144. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-0764-9_47
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DOI: https://doi.org/10.1007/978-1-4757-0764-9_47
Publisher Name: Springer, Boston, MA
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