Abstract
The function of class I genes mapping in the Q and TL regions of the MHC is unknown. Data presented in this study will show that two genes control a determinant (Qc-1) recognized by cloned cytotoxic T lymphocytes (CTL). One gene is a class I gene and maps in the Q region. The second gene maps in the K-end of the MHC and has tentatively been mapped between A ß3 and A ß2 with recombinant strains. Monoclonal antibody reactive with Q region encoded class I molecules blocks CTL recognition of the Qc-1 determinant at the target cell level. An attractive interpretation of these data is that the Q region class I molecule serves as a CTL restriction element for the recognition of a K-end encoded peptide.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Fischer-Lindahl, K., Loveland, B.E., and Richards, C.S.: The end of “H-2”? In C.S. David (ed):Major Histocompatibility Genes and Their Role in Immune Function, Plenum Publishing Corp, New York, in press.
Flaherty, L., Karl, M., and DiBiase, K.: The Qa series of antigens. In B. Pernis and H.J. Vogel (eds): Cell Biology of the Major Histocompatibility Complex, pp 229–237, Academic Press, New York, 1985.
Flavell, R.A., Allen, H., Burkly, L.C., Sherman, D.H., Waneck, G.L., and Widera, G.: Molecular biology of the H-2 histocompatibility complex. Science 233: 437–443, 1986.
Forman, J., Trial, J., Tonkonogy, S., and Flaherty, L.: The Qa-2 subregion controls the expression of two antigens recognized by H-2 unrestricted cytotoxic T cells. J. Exp. Med. 155: 749–767, 1982.
Homer, R.J. and Murphy, D.B.: Qc-1, a novel Q region controlled CTL determinant expressed on B lymphocytes. J. Immunol. 138: 4322–4328, 1987.
Klein, J.: Natural History of the Major Histocompatibility Complex, John Wiley und Sons, New York, 1986.
Lynes, M.A., Tonkonogy, S., and Flaherty, L.: Qa-1 and Qa-2 expression on CFU-s. J. Immunol. 129: 928–930, 1982.
Matzinger, R. and Bevan, M.: Why do so many lymphocytes respond to major histocompatibility antigens? Cell. Immunol. 29: 1–5, 1977.
Mellor, A.: The class I MHC gene family in mice. Immunology Today 7: 19–24, 1986.
Mellor, A., Antoniou, J., and Robinson, P.: Structure and expression of genes encoding murine Qa-2 class I antigens. Proc. Natl. Acad. Sci. U.S.A. 82: 5920–5924, 1985.
Monaco, J.J. and McDevitt, H.O.: The IMP antigens: a stable MHC-controlled multisubunit protein complex. Human Immunology 15: 416–426, 1986.
Ozato, K. and Sachs, D.H.: Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H=2b haplotype reveal genetic control of isotype expression. J. Immunol. 126: 317–321, 1981.
Sandrin, M., Hogarth, P., and McKenzie, I: Two “Qa” specificities Qa-m7 and Qa-m8 defined by monoclonal antibodies. J. Immunol. 131: 546–547, 1983.
Sharrow, S.O., Flaherty, L., and Sachs, D.H.: Serologic cross-reactivity between class I MHC molecules and an H-2-Linked differentiation antigen as detected by monoclonal antibodies. J. Exp. Med. 159: 21–40, 1984.
Steinmetz, M., Stephan, D., and Fischer-Lindahl, K: Gene organization and recombinational hot spots in the murine histocompatibility complex. Cell 44: 895–904, 1986.
Straus, D., Stroynowski, I., Schiffer, S., and Hood, L.: Expression of hybrid class I genes of the major histocompatibility complex of the mouse in L cells. Proc. Natl. Acad. Sci. U.S.A. 82: 6245–6249, 1985.
Stroynowski, I., Forman, J., Goodenow, R., Schiffer, S., McMillian, M., Sharrow, S., Sachs, D., and Hood, L.: Expression and T cell recognition of hybrid antigens with amino terminal domains encoded by Qa-2 region of major histocompatibility complex and carboxyl termini of transplantation antigens. J. Exp. Med. 161: 835–952, 1985.
Stroynowski, I., Soloski, M., Low, M., and Hood, L.: A single gene encodes soluble and membrane bound forms of the major histocompatibility Qa-2 antigen: Anchoring of the product by a phospholipid tail. Cell, in press.
van de Meugheuvel, W., van Seventer, G., and Demant, P.: A new Tla region antigen, Qa-11, similar to Qa-2 and associated with B type 82-microglobulin. J. Immunol. 134: 2507–2512, 1985.
Waneck, G.L., Sherman, D.H., Calvin, S., Allen, H., and Flavell, R.A.: Tissue specific expression of cell-surface Qa-2 antigen from a transfected Q_7b gene of C57BL/10 mice. J. Exp. Med. 165: 1358–1370, 1987.
Watts, T.H. and McConnell, H.M.: Biophysical aspects of antigen recognition by T cells. Ann. Rev. Imnunol. 5: 461–475, 1987.
Widacki, S.M., Flaherty, L., and Cook, R.G.: Biochemical characterization of the molecules reactive with Qa–6 antiserum and the monoclonal antibody 20–8–4: Evidence for structural similarity with the Qa–2 molecule. J. Immunol. 135: 3333 – 3339, 1985.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1987 Springer Science+Business Media New York
About this chapter
Cite this chapter
Murphy, D.B., Homer, R.J. (1987). A Q Region Product as a CTL Restriction Element?. In: David, C.S. (eds) H-2 Antigens. NATO ASI Series, vol 144. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-0764-9_35
Download citation
DOI: https://doi.org/10.1007/978-1-4757-0764-9_35
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4757-0766-3
Online ISBN: 978-1-4757-0764-9
eBook Packages: Springer Book Archive