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H-2 Antigens pp 297-304 | Cite as

Macrophage IA Hybrid Molecule as Product of the Ir-Thy-1 Genes

  • P. Zhou
  • L. J. Quackenbush
  • B. Albini
  • M. B. Zaleski
Part of the NATO ASI Series book series (NSSA, volume 144)

Abstract

A primary anti-Thy-1 response in mice is under complex genetic control (Zaleski et al. 1986). This control has been shown to involve either class I or class II H-2 molecules depending on the form in which the Thy-1 antigen is presented to a responder. Briefly, when thymocytes from an H-2-compatible donor are used, the cell-bound Thy-1 antigen seems to be presented directly to the responder’s T cells. The latter are believed to require simultaneous stimulation by the carrier-like or helper determinants (Lake and Douglas 1978) or acolytes (Klein and Zaleski 1987). In contrast, when thymocytes from H-2-incompatible donors are used, the cell-free Thy-1 antigen, which is shed from the immunizing thymocytes, appears to be presented by the responder’s macrophages. This presentation most likely involves associative recognition of the Thy-1 antigen and the product of the two complementary Ir-Thy-1 genes (Zaleski and Klein 1978). Recently, it was demonstrated that the product of these genes is identical with a hybrid IA molecule (Quackenbush et al. 1985, Zaleski et al. 1985). The working hypothesis outlined above is based on two crucial observations. First, a good anti-Thy-1 response to the cell-free antigen is demonstrable only in F1 hybrids that express a particular hybrid IA molecule (Quackenbush et al. 1985). Second, in vivo administration of monoclonal antibodies specific for a particular class II molecule inhibits the response to the cell-free, but not to the cell-bound antigen (Zaleski et al. 1985, Quackenbush et al. 1987).

Keywords

Inbred Strain Associative Recognition Splenic Macrophage Chloroquine Diphosphate Helper Determinant 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1987

Authors and Affiliations

  • P. Zhou
    • 1
  • L. J. Quackenbush
    • 1
  • B. Albini
    • 1
  • M. B. Zaleski
    • 1
  1. 1.Department of MicrobiologyState University of New York at BuffaloBuffaloUSA

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