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H-2 Antigens pp 275-281 | Cite as

Activation of I-A Gene Products by Aß Gene Transfection Using Bovine Papilloma Virus — Vector Systems

  • Kenji Okuda
  • Jun Fukushima
  • Susumu Kawamoto
  • Ichiro Aoki
  • Toyozoh Takahashi
Part of the NATO ASI Series book series (NSSA, volume 144)

Summary

The bovine papilloma virus (BPV) recombinant which carried class II MHC (A ß d ) gene (pBPAß-d) has been constructed at first. The recombinant plasmid was introduced into the mouse B cell hybridoma by co-transfection method with neomycin resistance gene. Uing this method we could impose into B cell hybridomas many copies of A ß d genes extrachromosomally. The several transfectants which had been introduced only beta chain gene of I-Ad have been revealed to expressed the I-Ad proteins on the cell surface by direct immunofluorescent technique. Moreover these I-Ad molecules could induce the IL-2 production of the I-Ad restricted autoreactive T cell hybridoma. So I-Ad molecules which express on the cell surface of the transfectants are fully functional to the T-cell response. RNA dot-blot analysis has revealed that the cells express not only A ß d gene but also Ad gene. Thus, A ß d gene transfection induced the expression of internally latent Ad gene by trans gene effect.

Keywords

Major Histocompatibility Complex Gene Transfection Neomycin Resistance Gene High Stringent Condition Trans Gene Effect 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1987

Authors and Affiliations

  • Kenji Okuda
    • 1
  • Jun Fukushima
    • 1
  • Susumu Kawamoto
    • 1
  • Ichiro Aoki
    • 2
  • Toyozoh Takahashi
    • 1
  1. 1.Departments of BacteriologyUniversity School of MedicineYokohamaJapan
  2. 2.Pathology, Yokohama CityUniversity School of MedicineYokohamaJapan

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