Structural and Functional Analysis of Murine Class II Molecules
Class II (Ia) major histocompatibility complex (MHC) molecules in the mouse are cell surface glycoproteins expressed on antigen presenting cells (APC; ref. 1). In the mouse two isotypic forms of Ia exist: the I-A and I-E molecules, each a heterodimer composed of noncovalently-associated a and ß chains. The association of foreign antigen, Ia molecules and the clonally distributed antigen receptor on T cells is critical in the initiation and regulation of immune responses1. However, the exact nature of the interaction between T cell receptor, antigen and Ia molecule is still poorly understood at the molecular level.
KeywordsMajor Histocompatibility Complex Immune Response Gene Polymorphic Residue Tertiary Conformation Eell Line
Unable to display preview. Download preview PDF.
- 3.R. N. Germain, J. D. Ashwell, R. I. Lechler, D. H. Margulies, K. M. Nickerson, G. Susuki, and J. Y. L. Tou, “Exon-shuffling” maps control of antibody-and T-cell-recognition sites to the NH2-terminal domain of the class II major histocompatibility polypeptide Al, Proc. Natl. Acad. Sci. USA 82: 2940 (1985).PubMedCrossRefPubMedCentralGoogle Scholar
- 9.B. N. Beck, L. H. Glimcher, A. E. Nilson, M. Pierres, and D. J. McKean, The structure-function relationship of I-A molecules: correlation of serologic and functional phenotypes of four I-Ak mutant cell lines, J. Tmmunol. 133: 3176 (1984).Google Scholar
- 13.I. J. Griffith, F. M. Carland, and L. H. Glimcher, Alteration of a non-polymorphic residue in a class II Eß gene eliminates an antibody-defined epitope without affecting T cell recognition, J. Immunol. 38: 4480 (1987).Google Scholar
- 21.G. Widera, and R. A. Flavell, The nucleotide sequence of the murine IEßb immune response gene: evidence for gene conversion events in class II genes of the major histocompatibility complex, EMBO J.Google Scholar
- 25.L. Mengle-Gaw, and H.O. McDevitt, Allelic variation in the murine la ß chain genes, in: “Regulation of the immune system”, H. Cantor, L. Chess, and E. Sercarz, eds., Alan R. Liss, Inc., NY, (1984).Google Scholar
- 26.G. G. Schlauder, M. P. Bell, B. N. Beck, A. Nilson, and D. J. McKean, The structure-function relationship of I-A molecules: a biochemical analysis of I-A polypeptides from mutant antigen-presenting cells and evidence of preferential association of allelic forms, J. Tmmunol. 135: 1945 (1985).Google Scholar