Guanidines pp 39-46 | Cite as

Enzymic Determination of Methylguanidine in Urine

  • Motoo Nakajima
  • Kazuo Nakamura
  • Yoshio Shirokane


Methylguanidine is known to accumulate in the body fluids of uremic patients1 , 2 and has proved, to be a uremic toxin3. Determination of its concentration in body fluids would, therefore, be useful in clinical practice. Recently, the determination of methylguanidine has been carried out by means of automated high-performance liquid chromatography with a fluorometric detection method for guanidino compounds4. The guanidino compounds analyzer is well suited for the simultaneous determination of several guanidino compounds. However, it is very expensive and inappropriate for analyses of many samples. In clinical practice, the enzymic determination of metabolite concentration has been widely employed because of its simplicity and specificity.


Chronic Renal Failure Enzymic Method Enzymic Determination Analytical Recovery Fluorometric Determination 


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Copyright information

© Springer Science+Business Media New York 1985

Authors and Affiliations

  • Motoo Nakajima
    • 1
  • Kazuo Nakamura
    • 1
  • Yoshio Shirokane
    • 1
  1. 1.Research & Development DivisionKikkoman CorporationNoda-shi, 278Japan

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