Guanidines pp 15-31 | Cite as

Automatic Guanidine Analyzer Using Benzoin as a Fluorogenic Reagent

  • Yiau-Lin Huang
  • Masaaki Kai
  • Yosuke Ohkura


In the current analytical methods for guanidino compounds, ion-exchange chromatographic methods coupled with automatic col­orimetric or fluorimetric detection, including high-performance liquid chromatography (HPLC), is the most popular because of its simplicity in operation, though other chromatographic methods based on paper1,2, thin-layer3,5 and gas chromatography5,8 have been reported. The colorimetric detection of these compounds by means of the Sakaguchi or Voges-Proskauer reaction in ion-exchange chromatography has a limited sensitivity and thus necessitates a large amount of sample9 11. On the other hand, fluorimetric detection based on the post-column derivatization in HPLC using ninhydrin or 9, 10-phenanthraquinone as a fluorogenic reagent can offer a method sensitive enough to measure the compounds at the picomole level12,14.


Potassium Hydroxide Citrate Dihydrate Potassium Perchlorate Trisodium Citrate Dihydrate Guanidino Compound 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. 1.
    A. S. Jones and T. W. Thompson, Detection of guanidine compounds on paper chromatograms, J. Chromatogr., 10: 248 (1963).PubMedGoogle Scholar
  2. 2.
    S. Giovannetti, M. Biagini and L. Cioni, Evidence that methyl-guanidine is retained in chronic renal failure, Experientia, 24: 341 (1968).PubMedCrossRefGoogle Scholar
  3. 3.
    M. Rink and D. Krebber, Dunnschichtchromatographische bestimmung von Kreatinin and Kreatin im harn, J. Chromatogr., 25: 80 (1966).PubMedCrossRefGoogle Scholar
  4. 4.
    F. Di Jeso, Qualitative and quantitative thin-layer chromatography of guanidine derivatives and differentiation of phosphagens from other phosphorus compounds, J. Chromatogr., 32: 269 (1968).PubMedCrossRefGoogle Scholar
  5. 5.
    A. Volkl and H. H. Berlet, Thin-layer chromatography of guanidino compounds, J. Clin. Chem. Clin. Biochem., 15: 267 (1977).PubMedGoogle Scholar
  6. 6.
    H. Patel and B. D. Cohen, Gas-liquid chromatographic measurement of guanidino acids, Clin. Chem., 21: 838 (1975).PubMedGoogle Scholar
  7. 7.
    T. Kawabata, H. Ohshima, T. Ishibuchi, M. Matsui and T. Kitsuwa, Gas chromatographic dtermination of methylguanidine, guanidine and agmatine as their hexafluoroacetylacetonates, J. Chromatogr., 140: 47 (1977).PubMedCrossRefGoogle Scholar
  8. 8.
    A. Mori, T. Ichimura and H. Matsumoto, Gas chromatography-mass spectrometry of guanidino compounds in brain, Anal. Biochem., 89: 393 (1978).PubMedCrossRefGoogle Scholar
  9. 9.
    P. P. Kamoun, J. M. Pleau and N. K. Man, Semiautomated method for measurement of guanidinosuccinic acid in serum, Clin. Chem., 18: 355 (1972).PubMedGoogle Scholar
  10. 10.
    R. Shainkin, Y. Berkenstadt, Y. Giatt and G. M. Berlyne, An automated technique for the analysis of plasma guanidino acids, and some findings in chronic renal disease, Clin. Chem. Acta, 60: 45 (1975).CrossRefGoogle Scholar
  11. 11.
    M. Matsumoto, H. Kishikawa and A. Mori, Guanidino compounds in the sera of uremic patients and in the sera and brain of experimental uremic rabbits, Biochem. Med., 16: 1 (1976).PubMedCrossRefGoogle Scholar
  12. 12.
    Y. Yamamoto, T. Manji, A. Saito, K. Maeda and K. Ohta, Ion exchange chromatographic separation and fluorimetric detection of guanidino compounds in physiologic fluids, J. Chromatogr., 162: 327 (1979).PubMedCrossRefGoogle Scholar
  13. 13.
    Y. Yamamoto, A. Saito, T. Manji, K. Maeda and K. Ohta, Quantitative analysis of methylguanidine and guanidine in physiologic fluid by high-performance liquid chromatography-fluorescence detection method, J. Chromatogr., 162: 23 (1979).PubMedCrossRefGoogle Scholar
  14. 14.
    Y. Hiraga and T. Kinoshita, Post-column derivatization of guanidino compounds in high-performance liquid chromatography using ninhydrin, J. Chromatogr., 226: 43 (1981).PubMedCrossRefGoogle Scholar
  15. 15.
    Y. Ohkura and M. Kai, Fluorimetric determination of mono-substituted guanidino compounds with benzoin-dimethylformamide reagent, Anal. Chim. Acta, 106: 89 (1979).CrossRefGoogle Scholar
  16. 16.
    M. Kai, T. Miura, K. Kohashi and Y. Ohkura, New method for the fluorimetric determination of guanidino compounds with benzoin, Chem. Pharm. Bull., 29: 1115 (1981).CrossRefGoogle Scholar
  17. 17.
    M. Kai, M. Yamaguchi and Y. Ohkura, Fluorescent products of the reaction of monosubstituted guanidino compounds with benzoin-dimethylformamide, Anal. Chim. Acta, 120: 411 (1980).CrossRefGoogle Scholar
  18. 18.
    M. Kai, T. Miyazaki, M. Yamaguchi and Y. Ohkura, High-performance liquid chromatography of guanidino compounds using benzoin as a pre-column fluorescent derivatization reagent, J. Chromatogr., 268: 417 (1983).CrossRefGoogle Scholar
  19. 19.
    D. J. Durzan, Automated chromatographic analysis of free monosubstituted guanidines in physiological fluids, Can. J. Biochem., 47: 657 (1969).PubMedCrossRefGoogle Scholar
  20. 20.
    R. E. Majors, Effect of particle size on column efficiency in liquid-solid chromatography, J. Chromatogr. Sci., 11: 88 (1973).Google Scholar
  21. 21.
    J. Bergström, P. Fûrst and L. Zimmerman, Uremic middle molecules exist and are biologically active, Clin. Nephrol., 11: 229 (1979).PubMedGoogle Scholar
  22. 22.
    J. Menyhart and J. Grof, Many hitherto unknown peptides are principal constituents of uremic middle molecules, Clin. Chem., 27: 1712 (1981).PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1985

Authors and Affiliations

  • Yiau-Lin Huang
    • 1
  • Masaaki Kai
    • 1
  • Yosuke Ohkura
    • 1
  1. 1.Faculty of Pharmaceutical SciencesKyushu UniversityHigashi-ku, Fukuoka 812Japan

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