Advertisement

Proliferative and Functional Impairment of Pancreatic Epithelial Cells Maintained in Vitro

  • Robert J. Hay
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 53)

Abstract

The use of animal cells in monolayer culture for model studies of the ageing process has increased extensively during recent years. In this regard, comparatively more attention has been directed towards the limited division potential of human fibroblasts than to limits in specific functional capability of this or other cell types with time in culture (1). This may be understandable when one considers the dearth of information concerning factors, environmental or otherwise, which affect expression of differentiated function by cells in culture. Nonetheless, for many experimental gerontologists, the age-associated loss of ability to synthesize tissue-specific products is thought to represent a major consequence of tissue ageing: one which, in most cases, cannot be accounted for by concomitant loss of individual cells (2,3).

Keywords

Acinar Cell Clonal Culture Pancreatic Epithelial Cell Embryonic Pancreas Human Amnion Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Reference

  1. 1.
    Cristofalo, V.J. 1972. Animal cell cultures as a model system for the study of aging. Adv. Gerontol. Res. 4: 45.Google Scholar
  2. 2.
    Adelman, R.C. 1971. Age-dependent effects in enzyme induction-a biochemical expression of aging. Exp. Gerontol. 6: 75.Google Scholar
  3. 3.
    Curtis, H.J. and Tilley, J. 1971. The life-span of dividing mammalian cells in vivo. J. Gerontol. 26: 1.PubMedCrossRefGoogle Scholar
  4. 4.
    Coon, I.G. 1966. Clonal stability and phenotypic expression of chick cartilage cells in vitro. Proc. Nat. Acad. Sci. USA 55:66.Google Scholar
  5. 5.
    Kaighn, M.E. and Prince, A.M. 1971. Production of albumin and other serum proteins by clonal cultures of normal human liver. Proc. Nat. Acad. Sci. USA 68: 2396.Google Scholar
  6. 6.
    Houck, J.C., Sharma, V.V. and Hayflick, L. 1971. Functional failures of cultured human diploid fibroblasts after continued population doublings. Proc. Soc. Exp. Biol. Med. 137: 331.Google Scholar
  7. 7.
    Macek, M., Hurych, J. and Chvapil, M. 1967. The collagen protein formation in tissue cultures of human diploid strains. Cytologia 32: 426.PubMedCrossRefGoogle Scholar
  8. 8.
    Cahn, R.D., Coon, H.G. and Cahn, M.B. 1966. Cell culture and cloning techniques. In: Methods in Developmental Biology. F.H. Tilt and.N.K. Wessells, eds., Crowell, p. 493.Google Scholar
  9. 9.
    Amsterdam, A. and Jamieson, J.D. 1972. Structural and func¬tional characterization of isolated pancreatic exocrine cells. Proc. Nat. Acad. Sci, USA 69: 3028.Google Scholar
  10. 10.
    Jamieson, A.D., Pruitt, K.M. and Caldwell, R.C. 1969. improved amylase assay. J. Dental Res. 48. 483.Google Scholar
  11. 11.
    Shear, M. and Pearse, A.G.E. 1963. A starch substrate film method for the histochemical localization of amylase. Exp. Cell Res. 32: 174.Google Scholar
  12. 12.
    Lowry, O,H., Rosebrough, N.J., Farr, A.L. and Randall, R.J. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193: 265.Google Scholar
  13. 13.
    Buckley, B., Rookstool, P. and Hay, R.J. Unpublished observations.Google Scholar
  14. 14.
    Hay, R.J., Yoshikawa -Fukada, M.,Yuyama, S. and Ebert, J.D. 1968. Cell differentiation and viral susceptibility. Carnegie Inst. Year Book 67: 429.Google Scholar
  15. 15.
    Parsa, I., Marsh,W.H, and Fitzgerald, P.J. 1972. Pancreas acinar cell differentiation. V. Significance of methyl groups in morphologic and enzymatic development. Exp. Cell Res. 73: 49.Google Scholar
  16. 16.
    Ronzio, R.A. and Rutter, W.J. 1973. Effects of a partially purified factor from chick embryos on macromolecular synthesis of embryonic pancreatic epithelia. Dev. Biol. 30: 307.Google Scholar
  17. 17.
    Wessels, N.K. and Cohen, J.H. 1967. Early pancreas organo-genesis: morphogenesis, tissue interactions, and mass effects. Dev. Biol. 15: 237.Google Scholar
  18. 18.
    Orei, L. Like, A.A., Amherdt, M., Blondel, B., Kanazawa Y., Marliss, E,B., Lambert, A.E., Wollheim, C.B. and Renold, A.E. 1973. Monolayer cell culture of neonatal rat pancreas: An ultrastructural and biochemical study of functioning endocrine cells. J. Ultrastruct. Res. 43: 270.Google Scholar
  19. 19.
    Chick, W.L., Lauris, V., Flewelling, J.H., Andrews, K.A. and Woodruff, J.M. 1973. Effects of glucose on beta cells in pancreatic monolayer.cultures. Endocrinol. 92: 212.CrossRefGoogle Scholar
  20. 20.
    Chang, R.S. 1962. Metabolic alterations with senescence of human cells: Some observations in vitro. Arch. Int. Med. 110: 563.Google Scholar

Copyright information

© Springer Science+Business Media New York 1975

Authors and Affiliations

  • Robert J. Hay
    • 1
  1. 1.Department of Biological SciencesWright State UniversityDaytonUSA

Personalised recommendations