Advertisement

The Effect of Hydrocortisone on DNA Synthesis and Cell Division during Aging in Vitro

  • V. J. Cristofalo
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 53)

Abstract

Several years ago, during the course of our studies on lysosomes in aging human diploid cells, we observed a small but consistent increase in the lifespan of cultures grown in the presence of 14μM hydrocortisone (HC).

Keywords

Glutamine Synthetase Label Nucleus Human Diploid Cell Hydrocortisone Effect Human Diploid Cell Strain 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Cristofalo, V.J. 1970. Metabolic aspects of aging in diploid human cells. In: Aging in Cell and Tissue Culture. E. Holeandkovä and V8 Cristofalo, eds., Plenum Press, N.Y., p. 83.Google Scholar
  2. 2.
    Cristofalo, V.J. 1972. Animal cell cultures as a model system for the study of aging. Adv. Gerontol. Res. 4: 45.Google Scholar
  3. 3.
    Cristofalo, V.J. 1973. Cellular Senescence: Factors modulating cell proliferation in vitro. Molec. Cell. Mech. Aging 27, 65.Google Scholar
  4. 4.
    Cristofalo, V.J. and Kabakjian, J. 1974. Lysosomal enzymes and aging in vitro. Mech. Aging and Develop, (in press).Google Scholar
  5. 5.
    Hayflick, L. and Moorhead, P. 1961. The serial cultivation of human diploid cell strains. Exp. Cell Res. 25: 585.PubMedCrossRefGoogle Scholar
  6. 6.
    Hayflick, L. 1965. The limited in vitro lifetime of human diploid cell strains. Exp. Cell Res. 37: 614.PubMedCrossRefGoogle Scholar
  7. 7.
    Cristofalo, V.J. and Sharf, B.B. 1973. Cellular senescence and DNA synthesis. Thymidine incorporation as a measure of population age in human diploid cells. Exp. Cell Res. 76: 419.PubMedCrossRefGoogle Scholar
  8. 8.
    Levine, E.M. 1972. Mycoplasma contamination of animal cell cultures: A simple, rapid detection method. Exp. Cell Res. 74: 99.Google Scholar
  9. 9.
    Volkin, E. and Cohn, W.E. 1954. Estimation of nucleic acids. Meth. Biochem. Anal. 1: 287.CrossRefGoogle Scholar
  10. 10.
    Färber, J., Rovera, G. and Baserga, R. 1971. Template activity of chromatin during stimulation of cellular proliferation in human diploid fibroblasts. Biochem. J. 122: 189.Google Scholar
  11. 11.
    Todaro, G.J., Lazar, G.K. and Green, H. 1965. The initiation of cell division in a contact-inhibited mammalian cell line. Comp. Physiol. 66: 325.Google Scholar
  12. 12.
    Turkington, R.W. and Topper, Y.J. 1967. Androgen inhibition of mammary gland differentiation in vitro Endocrinol. 80; 329.Google Scholar
  13. 13.
    Moscona, A.A. and Piddington, R. 1966» Stimulation by hydrocortisone of premature changes in the developmental pattern of glutamine synthetase in embryonic retina. Biochim. Biophys. Acta 121: 409.Google Scholar
  14. 14.
    Reif-Lehrer, L. and Chader, G. 1972. Hormonal effects on the neural retina: corticoid uptake, specific binding and structural requirements for the induction of glutamine synthetase. Biochim. Biophys. Acta 264: 186.Google Scholar
  15. 15.
    Viceps, D0 and Cristofalo, V.J. 1974. Glutamine synthetase activity in WI-38. Expt. Cell Res. (submitted for publication).Google Scholar
  16. 16.
    Ruhmann, A. Ga and Berliner, D.L. 1965. Effect of steroids on growth of mouse fibroblasts in vitro. Endocrinol. 76: 916.CrossRefGoogle Scholar
  17. 17.
    Arpels, C., Babcock, V.J. and Southam, C.M. 1964. Effect of steroids on human cell cultures; sustaining effect of hydrocortisone. Proc. Soc. Exp. Biol. Med, 115: 102.Google Scholar
  18. 18.
    Yuan, G.C. and Chang, R.S. 1969. Effect of hydrocortisone on age-dependent changes in lipid metabolism of primary human amnion cells in vitro. Proc. Soc. Exp. Biol. Med. 130: 934.Google Scholar
  19. 19.
    Yuan, G.C., Chang, R.S., Little, J.B. and Cornil, G. 1967. Prolongation of postmitotic lifespan of primary human amnion cells in vitro by hydrocortisone. J. Gerontol. 22: 174.PubMedCrossRefGoogle Scholar
  20. 20.
    Macieira-Coelho, A. 1966. Action of cortisone on human fibroblasts in vitro. Experientia 22: 390.PubMedCrossRefGoogle Scholar
  21. 21.
    Castor, C.W. and Prince, R.K. 1964. Modulation of the intrinsic viscosity of hyaluronic acid formed by human fibroblasts in vitro: The effects of hydrocortisone and colchicine. Biochim. Biophys. Acta 83: 165.Google Scholar
  22. 22.
    Smith, B.T., Torday, J.S. and Giroud, C.J.P. 1973. The growth promoting effect of Cortisol on human fetal lung cells. Steroids 22: 515.PubMedCrossRefGoogle Scholar
  23. 23.
    Donaldson, C. and Moorhead, P. 1974. Lifespan in vitro of fibroblasts supplemented with hydrocortisone: Thymidine incorporation and cell size. Exp. Cell Res. (submitted for publication).Google Scholar
  24. 24.
    Thrash, C.R. and Cunningham, D.D. 1973. Stimulation of division of density inhibited fibroblasts by glucocorticoids. Nature 242: 399.PubMedCrossRefGoogle Scholar
  25. 25.
    Armelin, H. 1973. Pituitary extracts and steroid hormones in the control of cell growth. Proc. Nat. Acad. Sci. 70: 2702.PubMedCrossRefGoogle Scholar
  26. 26.
    Gospodarowicz, D. 1974. Localisation of a fibroblast growth factor and its effect alone and with hydrocortisone on cell growth. Nature 249: 123.PubMedCrossRefGoogle Scholar
  27. 27.
    Bresciani, F. 1968. Topography of DNA synthesis in the mammary gland of the C3H mouse and its control by ovarian hormones; an autoradiographic study. Cell Tissue Kinet. 1: 51.Google Scholar

Copyright information

© Springer Science+Business Media New York 1975

Authors and Affiliations

  • V. J. Cristofalo
    • 1
  1. 1.Wistar Institute of Anatomy and BiologyPhiladelphiaUSA

Personalised recommendations