Abstract
The major advantage of cultured fibroblasts (CF) for the study of genetic regulatory aspects of insulin binding is the unrestricted use of stable diploid human cells several generations removed from neuro-humoral influences. CF were grown to confluence in 100 mm plastic dishes, 6–10 subcultures after harvesting from skin ex-plants. Donors included 11 nondiabetic controls aged three months to 70 years, four children two to nine years old with precocious tissue degeneration--three with Progeria (Rosenbloom and DeBusk, 1971) and one with Rothmund-Thomson Syndrome (Blinstrub, Lehman, and Steinberg, 1964)--and three persons ages 15, 19, and 30 years with lipoatrophic diabetes (LD). LD is a condition characterized by extremely high circulating insulin levels and resistance to the effects of massive doses of injected insulin (Flier, Kahn, Roth, and Bar, 1975).
Keywords
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
This work was carried out while ALR was supported by a Faculty Development Award from the University of Florida.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Blinstrub, R.S., Lehman, R., and Steinberg, T.H. (1964) Poikiloderma congenitale. Report of two cases. Arch. Derm. 89: 659–664.
DeMeyts, P., Roth, J., Neville, C.M., Gavin, J.R. III, and Lesniak, M.A. (1973) Insulin interactions with its receptors: Experimental evidence for negative cooperativity. Biochem. Biophys. Res. Commun. 55: 154–161.
DeMeyts,P., and Roth, J. (1975) Insulin receptors in the cell membrane: Quantitative description of the site-site interactions. Proc. of the 57th Annual Meeting of the Endocrine Society., p. 51.
Flier, J.S., Kahn, C.R., Roth, J., and Bar, R.S. (1975) Antibodies that impair insulin receptor binding in an unusual diabetic syndrome with severe insulin resistance. Science. 190: 63–65.
Hamlin, J.L. and Arguilla, E.R. (1974) Monoiodoinsulin: Preparation, purification, and characterization of a biologically active derivative substituted predominantly on Tyrosine A14. J. BioZ. Chem. 249:21–32.
Rosenbloom, A.L. and DeBusk, F.L. (1971) Progeria of Hutchinson-Gilford: A caricature of aging. Amer. Heart J. 82: 287–289.
Singal, D.P. and Goldstein, S. (1972) Absence of detectable HL-A antigens on cultured fibroblasts in progeria. J. Clin. Invest. 52: 2259–2263.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1978 Springer Science+Business Media New York
About this chapter
Cite this chapter
Rosenbloom, A.L., Goldstein, S., Yip, C.C. (1978). Insulin Binding by Cultured Fibroblasts from Normal and Insulin-Resistant Subjects. In: Klachko, D.M., Forte, L.R., Franz, J.M. (eds) Hormone Receptors. Advances in Experimental Medicine and Biology, vol 96. Springer, New York, NY. https://doi.org/10.1007/978-1-4757-0722-9_7
Download citation
DOI: https://doi.org/10.1007/978-1-4757-0722-9_7
Published:
Publisher Name: Springer, New York, NY
Print ISBN: 978-1-4757-0724-3
Online ISBN: 978-1-4757-0722-9
eBook Packages: Springer Book Archive