Insulin Binding by Cultured Fibroblasts from Normal and Insulin-Resistant Subjects
The major advantage of cultured fibroblasts (CF) for the study of genetic regulatory aspects of insulin binding is the unrestricted use of stable diploid human cells several generations removed from neuro-humoral influences. CF were grown to confluence in 100 mm plastic dishes, 6–10 subcultures after harvesting from skin ex-plants. Donors included 11 nondiabetic controls aged three months to 70 years, four children two to nine years old with precocious tissue degeneration--three with Progeria (Rosenbloom and DeBusk, 1971) and one with Rothmund-Thomson Syndrome (Blinstrub, Lehman, and Steinberg, 1964)--and three persons ages 15, 19, and 30 years with lipoatrophic diabetes (LD). LD is a condition characterized by extremely high circulating insulin levels and resistance to the effects of massive doses of injected insulin (Flier, Kahn, Roth, and Bar, 1975).
KeywordsInsulin Receptor Culture Fibroblast Insulin Binding Negative Cooperativity Nondiabetic Control
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