Evidence for the Role of Immobilization of Ligand-Occupied Membrane Receptors in Signal Transduction

  • David A. Jans
Part of the Molecular Biology Intelligence Unit book series (MBIU)


As we saw in chapter 6, immobilization of hormone-occupied receptors plays an impor­tant role in downregulating response subsequent to hormonal stimulation in GTP-binding protein activating systems.1–3 However, receptor immobilization also appears to play an important role in eliciting the stimulatory signal in other receptor systems. As will be dis­cussed below, these include tyrosine kinase receptors, as already hinted at in chapter 4, as well as receptors mediating binding to the cell substratum or cell-cell interaction. In these cases, immobilization of receptors through dimerization/aggregation and/or complexation with cytosolic, cytoskeletal or extracellular components appears to represent the primary stimulus triggering signal transduction. Receptor movement within the plane of the mem­brane before ligand engagement, however, is required to effect receptor dimerization/aggre­gation and/or bring receptors to the sites of interaction with extracellular components or components on the surfaces of other cells and hence is also essential to signal transduction in adhesion/cell-cell recognition responses.


Focal Contact Mobile Fraction Lateral Mobility Receptor Aggregation Fibronectin Receptor 
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  1. 1.
    Fahrenholz F, Jans DA, Peters R. Lateral mobility of the V1- and V2-receptors in plasma membranes: a role in signal transduction and receptor down-regulation. Colloques INSERM: Vasopressin 1991; 208: 49–56.Google Scholar
  2. 2.
    Jans DA. The mobile receptor hypothesis revisited: a mechanistic role for hormone receptor lateral mobility in signal transduction. Biochim Biophys Acta 1992; 1113: 271–276.PubMedCrossRefGoogle Scholar
  3. 3.
    Jans DA, Pavo I. A mechanistic role for polypeptide hormone receptor lateral mobility in signal transduction. Amino Acids 1995; 9: 93–109.Google Scholar
  4. 4.
    Levi A, Schechter Y, Neufeld EJ et al. Mobility, clustering and transport of nerve growth factor in embryonal sensory cells and in a sympathetic neuronal cell line. Proc Natl Acad Sci USA 1980; 77: 3469–3473.PubMedCrossRefGoogle Scholar
  5. 5.
    Schlessinger J, Schechter Y, Cuatrecasas P et al. Quantitative determination of the lateral diffusion coefficients of the hormone-receptor complexes of insulin and epidermal growth factor on the plasma membrane of cultured fibroblasts. Proc Natl Acad Sci USA 1978; 75: 5353–5357.PubMedCrossRefGoogle Scholar
  6. 6.
    Zidovetzki R, Yarden Y, Schlessinger J et al. Rotational diffusion of epidermal growth factor complexed to its surface receptor the rapid microaggregation and endocytosis of occupied receptors. Proc Natl Acad Sci USA 1981; 78: 6981–6985.PubMedCrossRefGoogle Scholar
  7. 7.
    Hillman GM, Schlessinger J. The lateral diffusion of epidermal growth factor complexed to its surface receptors does not account for the thermal sensitivity of patch formation and endocytosis. Biochemistry 1982; 21: 1667–1672.PubMedCrossRefGoogle Scholar
  8. 8.
    Rees AR, Gregoriou M, Johnson P et al. High-affinity epidermal growth factor receptors on the surface of A-431 cells have restricted lateral diffusion. EMBO J 1984; 3: 1843–1847.PubMedGoogle Scholar
  9. 9.
    Schlessinger J, Schechter Y, Willingham MC et al. Direct visualization of binding, aggregation, and internalization of insulin and epidermal growth factor on living fibroblastic cells. Proc Natl Acad Sci USA 1978; 75: 2659–2663.PubMedCrossRefGoogle Scholar
  10. 10.
    Zidovetzki R, Yarden Y, Schlessinger, J et al. Rotational diffusion of epidermal growth factor complexed to its surface receptor the rapid microaggregation and endocytosis of occupied receptors. Proc Natl Acad Sci USA 1981; 78: 6981–6985.PubMedCrossRefGoogle Scholar
  11. 11.
    Mock EJ, Niswender GD. Differences in the rates of internalization of 125I-labeled human chorionic gonadotropin, luteinizing hormone, and epidermal growth factor by ovine luteal cells. Endocrinology 1983; 113 (l): 259–264.PubMedCrossRefGoogle Scholar
  12. 12.
    Panaotou G, Waterfield MD. The assembly of signalling complexes by receptor tyrosine kinases. Bioessays 1993; 15 (3): 171–177.CrossRefGoogle Scholar
  13. 13.
    Ullrich A, Schlessinger J. Signal transduction by receptors with tyrosine kinase activity. Cell 1990; 61: 203–212.PubMedCrossRefGoogle Scholar
  14. 14.
    Noh DY, Shin SH, Rhee SG. Phosphoinositide-specific phospholipase C and mitogenic signaling. Biochim Biophys Acta 1995; 1242 (2): 99–113.PubMedGoogle Scholar
  15. 15.
    Honegger AM, Kris RM, Ullrich A et al. Evidence that autophosphorylation of solu-bilized EGF-receptors is mediated by inter-molecular cross phosphorylation. Proc Natl Acad Sci USA 1989; 86: 925–929.PubMedCrossRefGoogle Scholar
  16. 16.
    Honegger AM, Schmidt A, Ullrich A et al. Evidence for EGF-induced autophosphorylation of the EGF-receptor in living cells. Mol Cell Biol 1990; 10 (8): 4035–4044.PubMedGoogle Scholar
  17. 17.
    Ballotti R, Lammers R, Scimeca I-C et al. Intermolecular transphosphorylation between insulin receptors and EGF-insulin receptor chimerae. EMBO J 1989; 8: 3303–3309.PubMedGoogle Scholar
  18. 18.
    Gadella TW Jr, Jovin TM. Oligomeriza-tion of epidermal growth factor receptors on A431 cells studied by time-resolved fluorescence imaging microscopy. A stereochemical model for tyrosine kinase receptor activation. J Cell Biol 1995; 129 (6): 1543–1558.PubMedCrossRefGoogle Scholar
  19. 19.
    Sorokin A. Activation of the EGF receptor by insertional mutations in its juxtamembrane regions. Oncogene 1995; 11 (8): 1531–1540.PubMedGoogle Scholar
  20. 20.
    Gilboa L, Ben Levy R, Yarden Y et al. Roles for a cytoplasmic tyrosine and tyrosine kinase activity in the interactions of Neu receptors with coated pits. J Biol Chem 1995; 270 (13): 7061–7067.PubMedCrossRefGoogle Scholar
  21. 21.
    Weiner DB, Lui J, Cohen JA et al. A point mutation in the neu oncogene mimics ligand induction of receptor aggregation. Nature 1989; 339: 230–231.PubMedCrossRefGoogle Scholar
  22. 22.
    Heffetz D, Zick Y. Receptor aggregation is necessary for activation of the soluble insulin receptor kinase. J Biol Chem 1986; 261: 889–894.PubMedGoogle Scholar
  23. 23.
    Kahn CR, Baird KL, Jarrett DB et al. Direct demonstration that receptor crosslinking or aggregation is important in insulin action. Proc Natl Acad Sci USA 1978; 75 (9): 4209–4213.PubMedCrossRefGoogle Scholar
  24. 24.
    Fire E, Zwart DE, Roth MG et al. Evidence from lateral mobility studies for dynamic interactions of a mutant influenza hemagglutinin with coated pits. J Cell Biol 1991; 115: 1585–1594.PubMedCrossRefGoogle Scholar
  25. 25.
    Giugni TD, Braslau DL, Haigler HT. Electric field-induced redistribution and postfield relaxation of epidermal growth factor receptors on A431 cells. J Cell Biol 1987; 104 (5): 1291–1297.PubMedCrossRefGoogle Scholar
  26. 26.
    Venkatakrishnan G, McKinnon CA, Pilapil CG et al. Nerve growth factor receptors are preaggregated and immobile on responsive cells. Biochemistry 1991; 30 (11): 2748–2753.PubMedCrossRefGoogle Scholar
  27. 27.
    Ljungquist-Hoeddelius P, Lirvall M, Wasteson A et al. Lateral diffusion of PDGF-p receptor in human fibroblasts. Bioscience Reports 1991; ll(l):43–52.Google Scholar
  28. 28.
    Ljungquist P, Wasteson A, Magnusson K-E. Lateral diffusion of plasma membrane receptors labelled with either plate-let-derived growth factor (PDGF) or wheat germ agglutinin (WGA) in human leukocytes and fibroblasts. Bioscience Reports 1989; 9: 63–73.PubMedCrossRefGoogle Scholar
  29. 29.
    Yarden Y, Schlessinger J. Epidermal growth factor induces rapid, reversible aggregation of the purified epidermal growth factor receptor. Biochem 1987; 26: 1443–1451.CrossRefGoogle Scholar
  30. 30.
    Kahn CR. Membrane receptors for hormones and neurotransmitters. J Cell Biol 1976; 70: 261–286.PubMedCrossRefGoogle Scholar
  31. 31.
    Kahn CR. The molecular mechanism of insulin action. Annu Rev Med 1985; 36: 429–451.PubMedCrossRefGoogle Scholar
  32. 32.
    Cuatrecasas P. Membrane receptors. Annu Rev Biochem 1974; 43: 169–214.PubMedCrossRefGoogle Scholar
  33. 33.
    Fuchs R, Male P, Mellman I. Acidification and ion permeabilities of highly purified rat liver endosomes. J Biol Chem 1989; 264 (4): 2212–2220.PubMedGoogle Scholar
  34. 34.
    Contreras I, Caro J. The collisional hypothesis of insulin action, receptor internalization and diabetes. Trends in Biochem Sci 1989; 14: 399.CrossRefGoogle Scholar
  35. 35.
    Atlas D, Volsky DJ, Levitzki A. Lateral mobility of beta-receptors involved in adenylate cyclase activation. Biochim Biophys Acta 1980; 597 (1 ): 64–69.PubMedCrossRefGoogle Scholar
  36. 36.
    Jans DA, Peters R, Jans P et al. Vasopressin V2-receptor mobile fraction and ligand-dependent adenylate cyclase-activ-ity are directly correlated in LLC-PKj renal epithelial cells. J Cell Biol 1991; 114 (l): 53–60.PubMedCrossRefGoogle Scholar
  37. 37.
    Jans DA, Peters R, Jans P et al. Ammonium chloride affects receptor number and lateral mobility of the vasopressin V2-type receptor in the plasma membrane of LLC-PK! renal epithelial cells: role of the cytoskeleton. Exper Cell Res 1990; 191: 121–128.CrossRefGoogle Scholar
  38. 38.
    Zakharova OM, Rosenkranz AA, Sobolev AS. Modification of fluid lipid and mobile protein fractions of reticulocyte plasma membranes affects agonist-stimu-lated adenylate cyclase. Application of the percolation theory. Biochim Biophys Acta 1995; 1236: 177–184.PubMedCrossRefGoogle Scholar
  39. 39.
    Schlessinger J. Signal transduction by al-losteric receptor oligomerization. Trends Biochem Sci 1988; 13: 443–447.PubMedCrossRefGoogle Scholar
  40. 40.
    Schlessinger J. The epidermal growth factor receptor as a multifunctional allos-teric protein. Biochemistry 1989; 27: 3119–3123.CrossRefGoogle Scholar
  41. 41.
    Yarden Y, Schlessinger J. Self-phospho-rylation of epidermal growth factor: evidence for a model of intermolecular al-losteric activation. Biochem 1987; 26: 1434–1442.CrossRefGoogle Scholar
  42. 42.
    Metzger H, Kinet JP. How antibodies work: focus on Fc receptors. FASEB J 1988; 2 (1): 3–11.PubMedGoogle Scholar
  43. 43.
    Blank U, Ra C, Miller L et al. Complete structure and expression in transfected cells of high affinity IgE receptor. Nature 1989; 337 (6203): 187–189.PubMedCrossRefGoogle Scholar
  44. 44.
    Metzger H. The receptor with high affinity for IgE. Immunol Rev 1992; 125: 37–48.PubMedCrossRefGoogle Scholar
  45. 45.
    Fridman WH, Bonnerot C, Daeron M et al. Structural bases of Fey receptor functions. Immunol Rev 1992; 125: 49–76.PubMedCrossRefGoogle Scholar
  46. 46.
    Hogarth PM, Hulett MD, Ierino FL et al. Identification of the innumoglobulin binding regions (IBR) of FcyRII and FceRI. Immunol Rev 1992; 125: 21–35.PubMedCrossRefGoogle Scholar
  47. 47.
    Posner RG, Lee B, Conrad DH et al. Aggregation of IgE-receptor complexes on rat basophilic leukemia cells does not change the intrinsic affinity but can alter the kinetics of the ligand-IgE interaction. Biochemistry 1992; 31 (23): 5350–5356.PubMedCrossRefGoogle Scholar
  48. 48.
    Mao SY, Yamashita T, Metzger H. Chemical cross-linking of IgE-receptor complexes in RBL-2H3 cells. Biochemistry 1995; 34 (6): 1968–1977.PubMedCrossRefGoogle Scholar
  49. 49.
    Yamashita T, Mao SY, Metzger H. Aggregation of the high-affinity IgE receptor and enhanced activity of p53/56lyn protein-tyrosine kinase. Proc Natl Acad Sci USA 1994; 91 (23): 11251–11255.PubMedCrossRefGoogle Scholar
  50. 50.
    Zhang F, Yang B, Odin JA et al. Lateral mobility of Fey Rlla is reduced by protein kinase C activation. FEBS Lett 1995; 376 (l-2): 77–80.PubMedCrossRefGoogle Scholar
  51. 51.
    Trinchieri G, Valiante N. Receptors for the Fc fragment of IgG on natural killer cells. Nat Immun 1993; 12 (4–5): 218–234.PubMedGoogle Scholar
  52. 52.
    Rosales C, Brown EJ. Signal transduction by neutrophil immunoglobulin G Fc receptors. Dissociation of intracytoplasmic calcium concentration rise from inositol 1,4,5-trisphosphate. J Biol Chem 1992; 267 (8): 5265–5271.PubMedGoogle Scholar
  53. 53.
    Sarmay G, Rozsnyay Z, Koncz G et al. Interaction of signaling molecules with human Fey Rllbl and the role of various Fey Rllb isoforms in B-cell regulation. Immunol Lett 1995; 44 (2–3): 125–131.PubMedCrossRefGoogle Scholar
  54. 54.
    Apgar JR. Association of the crosslinked IgE receptor with the membrane skeleton is independent of the known signaling mechanisms in rat basophilic leukemia cells. Cell Regul 1991; 2 (3): 181–191.PubMedGoogle Scholar
  55. 55.
    Paolini R, Numerof R, Kinet JP. Phos-phorylation/dephosphorylation of high-affinity IgE receptors: a mechanism for coupling/uncoupling a large signaling complex. Proc Natl Acad Sci USA 1992; 89 (22): 10733–10737.PubMedCrossRefGoogle Scholar
  56. 56.
    Kent UM, Mao SY, Wofsy C et al. Dynamics of signal transduction after aggregation of cell-surface receptors: studies on the type I receptor for IgE. Proc Natl Acad Sci USA 1994; 91 (8): 3087–3091.PubMedCrossRefGoogle Scholar
  57. 57.
    Pribluda VS, Metzger H. Transmembrane signaling by the high-affinity IgE receptor on membrane preparations. Proc Natl Acad Sci USA 1992; 89 (23): 11446–11450.PubMedCrossRefGoogle Scholar
  58. 58.
    Wofsy C, Kent UM, Mao SY et al. Kinetics of tyrosine phosphorylation when IgE dimers bind to Fee receptors on rat basophilic leukemia cells. J Biol Chem 1995; 270 (35): 20264–20272.PubMedCrossRefGoogle Scholar
  59. 59.
    Li W, Deanin GG, Margolis B et al. Fee R1-mediated tyrosine phosphorylation of multiple proteins, including phospholi-pase Cy 1 and the receptor (3y2 complex, in RBL-2H3 rat basophilic leukemia cells. Mol Cell Biol 1992; 12 (7): 3176–3182.PubMedGoogle Scholar
  60. 60.
    Gergely J, Sarmay G. B-cell activation-induced phosphorylation of FcyRII: a possible prerequisite of proteolytic receptor release. Immunol Rev 1992; 125: 1–19.CrossRefGoogle Scholar
  61. 61.
    Ishizaka T, Ishizaka K. Triggering of histamine release from rat mast cells by divalent antibodies against IgE-receptors. J Immunol 1978; 120: 800–805.PubMedGoogle Scholar
  62. 62.
    Isersky C, Taurog JD, Poy G et al. Triggering of cultured mastocytoma cells by antibodies to the receptor for IgE. J Immunol 1978; 121: 549–558.PubMedGoogle Scholar
  63. 63.
    Posner RG, Subramanian K, Goldstein B et al. Simultaneous cross-linking by two nontriggering bivalent ligands causes synergistic signaling of IgE Fc epsilon RI complexes. J Immunol 1995; 155 (7): 3601–3609.PubMedGoogle Scholar
  64. 64.
    Menon AK, Holowka D, Webb WW et al. Clustering, mobility, and triggering activity of small oligomers of immunoglobulin E on rat basophilic leukemia cells. J Cell Biol 1986; 102: 534–540.Google Scholar
  65. 65.
    Menon AK, Holowka D, Webb WW et al. Cross-linking of receptor-bound IgE to aggregates larger than dimers leads to rapid immobilization. J Cell Biol 1986; 102: 541–550.PubMedCrossRefGoogle Scholar
  66. 66.
    Schlessinger J, Webb WW, Elson EL. Lateral motion and valence of Fc receptors on rat peritoneal mast cells. Nature 1976; 264: 550–552.PubMedCrossRefGoogle Scholar
  67. 67.
    Mao SY, Varin-Blank N, Edidin M et al. Immobilization and internalization of mutated IgE receptors in transfected cells. J Immunol 1991; 146 (3): 958–966.PubMedGoogle Scholar
  68. 68.
    McCloskey MA, Liu ZY, Poo MM. Lateral electromigration and diffusion of Fee receptors on rat basophilic leukemia cells: effects of IgE binding. J Cell Biol 1984; 99 (3): 778–787.PubMedCrossRefGoogle Scholar
  69. 69.
    Chang EY, Mao SY, Metzger H et al. Effects of subunit mutation on the rotational dynamics of Fc epsilon RI, the high affinity receptor for IgE, in transfected cells. Biochemistry 1995; 34 (18): 6093–6099.PubMedCrossRefGoogle Scholar
  70. 70.
    Myers JN, Holowka D, Baird B. Rotational motion of monomeric and dimeric immunoglobulin E-receptor complexes. Biochemistry 1992; 31 (2): 567–575.PubMedCrossRefGoogle Scholar
  71. 71.
    Poo H, Krauss JC, Mayo-Bond L et al. Interaction of Fey receptor type IIIB with complement receptor type 3 in fibroblast transfectants: evidence from lateral diffusion and resonance energy transfer studies. J Mol Biol 1995; 247 (4): 597–603.PubMedGoogle Scholar
  72. 72.
    Leedman PJ, Faulkner-Jones B, Cram DS et al. Cloning from the thyroid of a protein related to actin binding protein that is recognized by Graves disease immunoglobulins. Proc Natl Acad Sci USA 1993; 90 (13): 5994–5998.PubMedCrossRefGoogle Scholar
  73. 73.
    Mao SY, Alber G, Rivera J et al. Interaction of aggregated native and mutant IgE receptors with the cellular skeleton. Proc Natl Acad Sci USA 1992; 89 (l): 222–226.PubMedCrossRefGoogle Scholar
  74. 74.
    Robertson D, Holowka D, Baird B. Cross-linking of immunoglobulin E-receptor complexes induces their interaction with the cytoskeleton of rat basophilic leukemia cells. J Immunol 1986; 136: 4565–4572.PubMedGoogle Scholar
  75. 75.
    Jans DA, Peters R, Fahrenholz F. An inverse relationship between receptor internalization and the fraction of laterally mobile receptors for the vasopressin re-nal-type V2-receptor; an active role for receptor immobilization in down-regulation? FEBS Lett 1990; 274: 223–226.PubMedCrossRefGoogle Scholar
  76. 76.
    Maxfield FR, Willingham MC, Haigler HT et al. Binding, surface mobility, internalization, and degradation of rhodamine-labeled a2-macroglobulin. Biochemistry 1981; 20 (18): 5353–5358.PubMedCrossRefGoogle Scholar
  77. 77.
    Roettger BF, Rentsch RU, Hadac EM et al. Insulation of a G protein-coupled receptor on the plasmalemmal surface of the pancreatic acinar cell. J Cell Biol 1995; 130: 579–590.PubMedCrossRefGoogle Scholar
  78. 78.
    Carraway III KL, Koland JG, Cerione RA. Visualization of epidermal growth factor (EGF) receptor aggregation in plasma membranes by fluorescence energy transfer. J Biol Chem 1989; 264: 8699–8707.PubMedGoogle Scholar
  79. 79.
    Furuichi K, Rivera J, Isersky C. The fate of IgE bound to rat basophilic leukemia cells. III. Relationship between antigen-induced endocytosis and serotonin release. J Immunol 1984; 133: 1513–1520.PubMedGoogle Scholar
  80. 80.
    Ra C, Furuichi K, Mullins JM et al. Internalization of IgE receptors on rat basophilic leukemia cells by phorbol ester: comparison with endocytosis induced by receptor aggregation. Eur J Immunol 1989; 19: 1771–1777.PubMedCrossRefGoogle Scholar
  81. 81.
    Furuichi K, Rivera J, Triche T et al. The fate of IgE bound to rat basophilic leukemia cells. IV. Functional association between the receptors for IgE. J Immunol 1985; 134: 1766–1773.PubMedGoogle Scholar
  82. 82.
    Becker KE, Ishizaka T, Metzger H et al. Surface IgE on human basophils during histamine release. J Exp Med 1973; 138: 394–409.PubMedCrossRefGoogle Scholar
  83. 83.
    Lawson D, Fewtrell C, Gomperts B et al. Anti-immunoglobulin-induced histamine secretion by rat peritoneal mast cells studied by immunoferritin electron microscopy. J Exp Med 1975; 142 (2): 391–402.PubMedCrossRefGoogle Scholar
  84. 84.
    Magro AM, Alexander A. Histamine-re-lease–in vitro studies of inhibitory region of dose-response curve. J Immunol 1974; 112: 1762–1765.PubMedGoogle Scholar
  85. 85.
    Springer TA. Adhesion receptors of the immune system. Nature 1990; 346: 425–434.PubMedCrossRefGoogle Scholar
  86. 86.
    Liu SJ, Hahn WC, Bierer BE et al. Intracellular mediators regulate CD2 lateral diffusion and cytoplasmic Ca2+ mobilization upon CD2-mediated T cell activation. Biophys J 1995; 68 (2): 459–470.PubMedCrossRefGoogle Scholar
  87. 87.
    Chan PY, Lawrence MB, Dustin ML et al. Influence of receptor lateral mobility on adhesion strengthening between membranes containing LFA-3 and CD2. J Cell Biol 1991; 115 (l): 245–255.PubMedCrossRefGoogle Scholar
  88. 88.
    Carpen O, Dustin ML, Springer TA et al. Motility and ultrastructure of large granular lymphocytes on lipid bilayers reconstituted with adhesion receptors LFA-1, ICAM-1, and two isoforms of LFA-3. J Cell Biol 1991; 115 (3): 861–871.PubMedCrossRefGoogle Scholar
  89. 89.
    Liu SJ, Golan DE. Mobilization of intracellular free Ca2+ upon T cell activation via TCR/CD3 is required to induce a decrease in CD2 lateral mobility in Jurkat T cell membranes. Biophys J 1994; 66: 149a.Google Scholar
  90. 90.
    Liu SJ, Golan DE. Regulation of CD2 lateral mobility by calmodulin and calcineurin in Jurkat T cell membranes. Biophys J 1994; 66: 149a.Google Scholar
  91. 91.
    Blotta MH, Marshall JD, DeKruyff RH et al. Cross-linking of the CD40 ligand on human CD4+ T lymphocytes generates a costimulatory signal that up-regu-lates IL-4 synthesis. J Immunol 1996; 156 (9): 3133–3140.PubMedGoogle Scholar
  92. 92.
    Arulanandam AR, Koyasu S, Reinherz EL. T cell receptor-independent CD2 signal transduction in FcR+ cells. J Exp Med 1991; 173 (4): 859–868.PubMedCrossRefGoogle Scholar
  93. 93.
    Pal R, Nair BC, Hoke GM et al. Lateral diffusion of CD4 on the surface of a human neoplastic T-cell line probed with a fluorescent derivative of the envelope glycoprotein (gpl20) of human immunodeficiency virus type 1 (HIV-1). J Cell Physiol 1991; 147 (2): 326–332.PubMedCrossRefGoogle Scholar
  94. 94.
    Grebenkamper K, Tosi PF, Lazarte JE et al. Modulation of CD4 lateral mobility in intact cells by an intracellularly applied antibody. Biochem J 1995; 312 (1 ): 251–259.PubMedGoogle Scholar
  95. 95.
    Bierer BE, Golan DE, Brown CS et al. A monoclonal antibody to LFA-3, the CD2 ligand, specifically immobilizes major histocompatibility complex proteins. Eur J Immunol 1989; 19 (4): 661–665.PubMedCrossRefGoogle Scholar
  96. 96.
    Corcao G, Sutcliffe RG, Kusel JR et al. Lateral diffusion of human CD2 wild type and mutants with large deletions in the transmembrane domain. Biochem Biophys Res Commun 1995; 208: 1131–1136.PubMedCrossRefGoogle Scholar
  97. 97.
    Sleckman BP, Shin J, Igras VE et al. Disruption of the CD4-p56lck complex is required for rapid internalization of CD4. Proc Natl Acad Sci USA 1992; 89: 7566–7570.PubMedCrossRefGoogle Scholar
  98. 98.
    Chopra H, Hatfield JS, Chang YS et al. Role of tumor cell cytoskeleton and membrane glycoprotein IRgpIIb/IIIa in platelet adhesion to tumor cell membrane and tumor cell-induced platelet aggregation. Cancer Res 1988; 48: 3787–3800.PubMedGoogle Scholar
  99. 99.
    Jaffe SH, Friedlander F, Matsuzaki F et al. Differential effects of the cytoplasmic domains of cell adhesion molecules on cell aggregation and sorting-out. Proc Natl Acad Sci USA 1990; 87: 3589–3593.PubMedCrossRefGoogle Scholar
  100. 100.
    Carpen O, Pallai P, Staunton DE et al. Association of intercellular adhesion molecule-1 (ICAM-1) with actin-con-taining cytoskeleton and alpha-actinin. J Cell Biol 1992; 118: 1223–1234.PubMedCrossRefGoogle Scholar
  101. 101.
    Burridge K. Substrate adhesions in normal and transformed fibroblasts: organization and regulation of cytoskeletal, membrane, and extracellular matrix components at focal contacts. Cancer Rev 1987; 4: 18–78.Google Scholar
  102. 102.
    Kupfer A, Singer SJ. The specific interaction of helper T-cells and antigen-presenting B cells. IV. Membrane and cytoskeletal reorganizations in the bound T cell as a function of antigen dose. J Exp Med 1989; 170: 1697–1713.PubMedCrossRefGoogle Scholar
  103. 103.
    Freed BM, Lempert N, Lawrence DA. The inhibitory effects of N- ethylmaleimide, colchicine and cytocha-lasins on human T-cell functions. Int J Immunopharmacol 1989; 11: 459–465.PubMedCrossRefGoogle Scholar
  104. 104.
    Ishijima SA, Asakura H, Suzuta T. Participation of cytoplasmic organelles in E- rosette formation. Immunol Cell Biol 1991; 69: 403–409.PubMedCrossRefGoogle Scholar
  105. 105.
    Namba Y, Ito M, Zu Y et al. Human T- cell L-plastin bundles actin filaments in a calcium-dependent manner. J Biochem (Tokyo) 1992; 112: 503–507.Google Scholar
  106. 106.
    Pacaud M, Derancourt I. Purification and further characterization of macrophage 70-kDa protein, a calcium-regulated, actin-binding protein identical to L- plastin. Biochemistry 1993; 32: 3448–3455.PubMedCrossRefGoogle Scholar
  107. 107.
    Ledbetter JA, Rabinovitch PS, Hellstrom I et al. Role of CD2 cross-linking in cytoplasmic calcium responses and T cell activation. Eur J Immunol 1988; 18: 1601–1608.PubMedCrossRefGoogle Scholar
  108. 108.
    Ferguson LM, Dustin ML, Chan P-Y et al. Redistribution of GPI-linked LFA-3 to regions of contact between LFA-3 reconstituted planar bilayer membranes and CD2+ T-lymphoblasts. J Cell Biol 1991; 115: 71a.CrossRefGoogle Scholar
  109. 109.
    McCloskey MA, Poo MM. Contact-induced restribution of specific membrane components: local accumulation and development of adhesion. J Cell Biol 1986; 102: 2185–2196.PubMedCrossRefGoogle Scholar
  110. 110.
    Bell GI. Models for the specific adhesion of cells to cells: a theoretical framework for adhesion mediated by reversible bonds between cell surface molecules. Science 1978; 200: 618–627.PubMedCrossRefGoogle Scholar
  111. 111.
    Bell GI. Theoretical models for cells-cell interactions in immune responses. Dev Cell Biol 1979; 4: 371–392.Google Scholar
  112. 112.
    Helmreich EJM, Elson EL. Protein and lipid mobility. Adv in Cyclic Nucleotide and Prot Phosphor Res 1984; 18: 1–62.Google Scholar
  113. 113.
    Duband J-L, Nuckolls GH, Ishihara A et al. Fibronectin receptor exhibits high lateral mobility in embryonic locomoting cells but is immobile in focal contacts and fibrillar streaks in stationary cells. J Cell Biol 1988; 107: 1385–1396.PubMedCrossRefGoogle Scholar
  114. 114.
    Duband JL. Extracellular matrix and embryonal morphogenesis: role of fibronectin in cell migration. Reprod Nutr Dev 1990; 30 (3): 379–395.PubMedCrossRefGoogle Scholar
  115. 115.
    Thiery JP, Duband J-L, Tucker GC. Cell migration in the vertebrate embryo. Annu Rev Cell Biol 1985; 1: 91–113.PubMedCrossRefGoogle Scholar
  116. 116.
    Zetter BR. Adhesion molecules in tumor metastasis. Semin Cancer Biol 1993; 4 (4): 219–229.PubMedGoogle Scholar
  117. 117.
    McCarthy JB, Basara ML, Palm SL et al. The role of cell adhesion proteins–laminin and fibronectin–in the movement of malignant and metastatic cells. Cancer Metastasis Rev 1985; 4 (2): 125–152.PubMedCrossRefGoogle Scholar
  118. 118.
    Mecham RP. Receptors for laminin on mammalian cells. FASEB J 1991; 5 (11): 2538–2546.PubMedGoogle Scholar
  119. 119.
    Albelda SM, Buck CA. Integrins and other cell adhesion molecules. FASEB J 1990; 4 (11): 2868–2880.PubMedGoogle Scholar
  120. 120.
    Sonnenberg A. Integrins and their ligands. Curr Top Microbiol Immunol 1993; 184: 7–35.PubMedCrossRefGoogle Scholar
  121. 121.
    Tucker RP, Edwards BF, Erickson CA. Tension in the culture dish: microfilament organization and migratory behaviour of quail neural crest cells. Cell Motil 1985; 5: 225–237.PubMedCrossRefGoogle Scholar
  122. 122.
    Shimizu Y, van-Seventer GA, Horgan KJ et al. Roles of adhesion molecules in T- cell recognition: fundamental similarities between four integrins on resting human T cells (LFA-1, VLA-4, VLA-5, VLA-6) in expression, binding, and costimulation. Immunol Rev 1990; 114: 109–143.PubMedCrossRefGoogle Scholar
  123. 123.
    Buck CA, Horwitz AF. Integrin, a transmembrane glycoprotein complex mediating cell-substratum adhesion. J Cell Sci Suppl 1987; 8: 237–250.Google Scholar
  124. 124.
    Schlessinger J, Barak LS, Hammes GG et al. Mobility and distribution of a cell surface glycoprotein and its interaction with other membrane components. Proc Natl Acad Sci USA 1977; 74 (7): 2909–2913.PubMedCrossRefGoogle Scholar
  125. 125.
    Horwitz A, Duggan K, Buck C et al. Interaction of plasma membrane fibronectin receptor with talin. A transmembrane linkage. Nature 1986; 320: 531–532.PubMedCrossRefGoogle Scholar
  126. 126.
    Burridge K, Molony L, Kelly T. Adhesion plaques: sites of transmembrane interaction between the extracellular matrix and the actin cytoskeleton. J Cell Sci Suppl 1987; 8: 211–229.PubMedGoogle Scholar
  127. 127.
    Kreis TE, Geiger B, Schlessinger J. Mobility of microinjected rhodamine actin within living chicken gizzard cells determined by fluorescence photobleaching recovery. Cell 1982; 29: 835–845.PubMedCrossRefGoogle Scholar
  128. 128.
    Stickel SK, Wang Y-L. Alpha-actinin-containing aggregates in transformed cells are highly dynamic structures. J Cell Biol 1987; 101: 1521–1526.CrossRefGoogle Scholar
  129. 129.
    Myohanen HT, Stephens RW, Hedman K et al. Distribution and lateral mobility of the urokinase-receptor complex at the cell surface. J Histochem Cytochem 1993; 41 (9): 1291–1301.PubMedCrossRefGoogle Scholar
  130. 130.
    Gudewicz PW, Gilboa N. Human uroki-nase-type plasminogen activator stimulates chemotaxis of human neutrophils. Biochem Biophys Res Commun 1987; 147: 1176–1181.PubMedCrossRefGoogle Scholar
  131. 131.
    Fibbi G, Ziche M, Morbidelli M et al. Interaction of urokinase with specific receptors stimulates mobilization of bovine adrenal capillary endothelial cells. Exp Cell Res 1988; 179: 385–395.PubMedCrossRefGoogle Scholar
  132. 132.
    Del Rosso M, Fibbi G, Dini G et al. Role of specific membrane receptors in uroki-nase-dependent migration of human keratinocytes. J Invest Dermatol 1990; 94: 310–316.PubMedCrossRefGoogle Scholar
  133. 133.
    Ossowski L, Clunie G, Masucci MT et al. In vivo interaction between urokinase and its receptor: effect on tumor cell invasion. J Cell Biol 1991; 115: 1107–1112.PubMedCrossRefGoogle Scholar
  134. 134.
    Bruckner A, Filderman AE, Kirchheimer JC et al. Endogenous receptor-bound urokinase mediates tissue invasion of the human lung carcinoma cell lines A549 and Calu-1. Cancer Res 1992; 52: 3043–3047.PubMedGoogle Scholar
  135. 135.
    Kobayashi H, Ohi H, Sugimura M et al. Inhibition of in vitro ovarian cancer cell invasion of modulation of urokinase type plasminogen activator and cathepsin B. Cancer Res 1992; 52: 3610–3614.PubMedGoogle Scholar
  136. 136.
    Griffin Jnr FM, Mullinax PJ. Augmentation of macrophage complement receptor function in vitro. III. C3b receptors that promote phagocytosis migrate within the plane of macrophage plasma membrane. J Med Exp 1981; 154: 291–305.CrossRefGoogle Scholar
  137. 137.
    Hermanowski-Vosatka A, Detmers PA, Gotze O et al. Clustering of ligand on the surface of a particle enhances adhesion to receptor-bearing cells. J Biol Chem 1988; 263 (33): 17822–17827.PubMedGoogle Scholar
  138. 138.
    Ross GD, Reed W, Dalzell JG et al. Macrophage cytoskeletal association with CR3 and CR4 regulates receptor mobility and phagocytosis of iC3b-opsonized erythrocytes. J Leukoc Biol 1992; 51 (2): 109–117.PubMedGoogle Scholar
  139. 139.
    Detmers PA, Wright SD, Olsen E et al. Aggregation of complement receptors on human neutrophils in the absence of ligand. J Cell Biol 1987; 105: 1137–1145.PubMedCrossRefGoogle Scholar
  140. 140.
    Pryzwansky KB, Wyatt T, Reed W et al. Phorbol ester induces transient focal concentrations of functional, newly expressed CR3 in neutrophils at sites of specific granule exocytosis. Eur J Cell Biol 1991; 54 (l): 61–75.PubMedGoogle Scholar
  141. 141.
    Griffin FM Jr, Mullinax PJ. Effects of differentiation in vivo and of lymphok-ine treatment in vitro on the mobility of C3 receptors of human and mouse mononuclear phagocytes. J Immunol 1985; 135 (5): 3394–3397.PubMedGoogle Scholar
  142. 142.
    Griffin FM Jr, Mullinax PJ. High concentrations of bacterial lipopolysaccharide, but not microbial infection-induced inflammation, activate macrophage C3 receptors for phagocytosis. J Immunol 1990; 145 (2): 697–701.PubMedGoogle Scholar
  143. 143.
    Detmers PA, Powell DE, Walz A et al. Differential effects of neutrophil-activat-ing peptide l/IL-8 and its homologues on leukocyte adhesion and phagacytosis. J Immunol 1991; 147 (12): 4211–4217.PubMedGoogle Scholar
  144. 144.
    Vedder NB, Harlan JM. Increased surface expression of CD lib/CD 18 (Mac-1) is not required for stimulated neutrophil adherence to cultured endothelium. J Clin Invest 1988; 81: 676–682.PubMedCrossRefGoogle Scholar
  145. 145.
    Siu K, Detmers PA, Levin SM et al. Transient adhesion of neutrophils to endothelium. J Exp Med 1989; 169: 1779–1793.CrossRefGoogle Scholar

Copyright information

© R.G. Landes Company 1997

Authors and Affiliations

  • David A. Jans
    • 1
  1. 1.John Curtin School of Medical ResearchAustralian National UniversityCanberraAustralia

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