The Use of Tritium and 14C Labelled Ethanol in Studies of Ethanol Metabolism at High Ethanol Concentrations
Measurement of the rate of ethanol metabolism of the liver at concentrations at which the pharmacological actions of ethanol become manifest, i.e. 50–80 mM are of considerable importance. Such measurements can, however, not be performed with acceptable accuracy by determination of the decrease in ethanol concentration in preparations such as slices, isolated hepatocytes, or perfused liver, as the concentration differences are small compared to the absolute level. In the intact organism the overall metabolism of ethanol at high concentrations can be determined by measurement of the blood alcohol concentration at suitable intervals, but in this case we cannot decide in which organ the metabolism takes place. Extra-hepatic metabolism may play a more important role at high than at low ethanol concentrations. The obvious solution to this problem is to measure the products formed from ethanol, not the disappearance of the substrate.
KeywordsEthanol Concentration Isotope Effect Blood Alcohol Concentration Perfusion Medium Carbon Water
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