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Special Techniques for Weighing Microgram Quantities of Tissue and Assaying Them for Enzyme Activities

  • Charles L. Rutherford

Abstract

To carry out biochemical analyses on single hydra or parts of an animal and to relate those measurements to the dry weight of the tissue analyzed. There are three essential steps: (1) the quick freeze-drying of the hydra; (2) the dissection and weighing of pieces of dry hydra tissue as light as 0.1 μg; (3) the biochemical assay of those dried tissues.

Keywords

Copper Wire Rubber Band Ocular Micrometer Pyridine Nucleotide Quartz Fiber 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Burt, A. M. 1966. Modification of the Lowry quartz fiber “Fishpole” ultramicrobalance. Microehern. J. 11: 18–25.Google Scholar
  2. Lowry, O. H. 1941. A quartz fiber balance. J. Biot Chem. 140: 183–189.Google Scholar
  3. Lowry, O. H., and Passonneau, J. V. 1972. A Flexible System of Enzymatic Analysis. Academic Press, New York.Google Scholar
  4. Lowry, O. 1-L, Roberts, N. R, and Kapphahn, J. L. 1957. The fluorometric measurement of pyridine nucleotides./ Biol. Chem. 224: 1041–1064.Google Scholar
  5. Rutherford, C. L. 1976. Glycogen degradation during migration of presumptive cell types in. Dictyostelium discoideunz. Biochim. Biophys. Acta. 74: 179–222.Google Scholar
  6. Rutherford, C. L., and Lenhoff. H. M. 1969. Enzymes of glucose catabolism in Hydra. H. Application of microfluorometric analysis to patterns of enzyme localization. Arch. Biochem. Biophys. 133: 128–136.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1983

Authors and Affiliations

  • Charles L. Rutherford
    • 1
  1. 1.Biology DepartmentVirginia Polytechnic Institute and State UniversityBlacksburgUSA

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