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Special Techniques for Weighing Microgram Quantities of Tissue and Assaying Them for Enzyme Activities

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Abstract

To carry out biochemical analyses on single hydra or parts of an animal and to relate those measurements to the dry weight of the tissue analyzed. There are three essential steps: (1) the quick freeze-drying of the hydra; (2) the dissection and weighing of pieces of dry hydra tissue as light as 0.1 μg; (3) the biochemical assay of those dried tissues.

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References

  • Burt, A. M. 1966. Modification of the Lowry quartz fiber “Fishpole” ultramicrobalance. Microehern. J. 11: 18–25.

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  • Rutherford, C. L., and Lenhoff. H. M. 1969. Enzymes of glucose catabolism in Hydra. H. Application of microfluorometric analysis to patterns of enzyme localization. Arch. Biochem. Biophys. 133: 128–136.

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Author information

Authors and Affiliations

  1. Biology Department, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, USA

    Charles L. Rutherford

Authors
  1. Charles L. Rutherford
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Editor information

Editors and Affiliations

  1. Department of Developmental and Cell Biology, University of California, 92717, Irvine, California, USA

    Howard M. Lenhoff

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© 1983 Springer Science+Business Media New York

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Rutherford, C.L. (1983). Special Techniques for Weighing Microgram Quantities of Tissue and Assaying Them for Enzyme Activities. In: Lenhoff, H.M. (eds) Hydra: Research Methods. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-0596-6_50

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  • DOI: https://doi.org/10.1007/978-1-4757-0596-6_50

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4757-0598-0

  • Online ISBN: 978-1-4757-0596-6

  • eBook Packages: Springer Book Archive

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