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Dissolving the Nematocyst Capsule Wall and Identifying its Protein Component(s)

  • Richard S. Blanquet

Abstract

Since the properties of the nematocyst capsule and thread undoubtedly play a significant role in the mechanism of nematocyst discharge, solubilization of these structures facilitates the chemical and physical analyses of some of their components. Analyses show that nematocysts contain a collagenlike protein (Lenhoff et al, 1957) linked by disulfide bonds (Blanquet and Lenhoff, 1966).

Keywords

Squash Preparation Electrophoretic Technique Procedure Qualitative Analysis Nematocyst Type Thiol Solution 
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References

  1. Blanquet, R., and Lenhoff, H. M. 1966. A disulfide-linked collagenous protein of nematocyst capsules. Science 154: 152–153.PubMedCrossRefGoogle Scholar
  2. Cleland, W. W. 1964. Dithiothreitol, a new protective reagent for SH groups, Biochemistry 3(4):480–482Google Scholar
  3. Davis, B. J. 1964. Disc electrophoresis. II. Method and application to human serum proteins. Ann. N.Y Acad. Sci, 121: 404–427.CrossRefGoogle Scholar
  4. Fishman, L., and Levy, M. 1967. Studies on the nematocyst capsule protein from the sea anemone Metridium marginatum, Biol. Bull. Woods Hole Mass. 133:464–465Google Scholar
  5. Gordon, A. H. 1972. Electrophoresis of proteins in polyacrylamide and starch gels. Ir.: Laboratory Techniques in Biochemistry and Molecular Biology, vol. 1, part 1 ( T. S. Work and E. Work, eds.), North Holland, Amsterdam.Google Scholar
  6. Lenhoff, H. M., Kline, E. S., and Hurley, R. 1957. A hydroxyproline-rich collagen-like protein of Hydra nematocysts. Biochim. Biophys. Acta 26: 204–205.PubMedCrossRefGoogle Scholar
  7. Mariscal, R N. 1971. Effect of a disulfide reducing agent on the nematocyst capsules from some Coelenterates, with an illustrated key to nematocyst classification. in: Experimental Coelenterate Biology ( H. M. Lenhoff, L. Muscatine, and L. V. Davis, eds.), University of Hawaii Press, Honolulu, pp. 157–168.Google Scholar
  8. Yanagita, T. M. 1959. Physiological mechanism of nematocyst responses in sea anemone. 1. Effects of trypsin and thioglycolate upon isolated nematocysts..fpn. J. Zool. 12: 361–375.Google Scholar

Copyright information

© Springer Science+Business Media New York 1983

Authors and Affiliations

  • Richard S. Blanquet
    • 1
  1. 1.Department of BiologyGeorgetown UniversityUSA

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