Eliminating All Nonepithelial Cells Using Colchicine

  • Beverly A. Marcum
  • Richard D. Campbell


To eliminate the interstitial cell population of hydra using colchicine treatments (Campbell, 1976; Marcum and Campbell, 1978). The resulting viable animals consist only of two layers of epithelial cells plus the noncellular mesoglea. These animals are termed epithelial hydra.


Interstitial Cell Cellular Composition Colchicine Treatment Colchicine Concentration Body Column 
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  1. Campbell, R. D. 1976. Eliminatio of Hydra interstitial and nerve cells by means of colchicine. J. Cell Sci. z/:1—B.Google Scholar
  2. David, C. N. 1973. A quantitative method for niaceration of hydra tissue, Wilhelm Roux.4 reh. axwicúmngsmoacnrg. 171u59–268.Google Scholar
  3. Marcum, B. A., and Campbell, R. D. 1978. Development of hydra lacking nerve and interstitialGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1983

Authors and Affiliations

  • Beverly A. Marcum
    • 1
  • Richard D. Campbell
    • 2
  1. 1.Department of Biological SciencesCalifornia State UniversityChicoUSA
  2. 2.Department of Developmental and Cell BiologyUniversity of CaliforniaIrvineUSA

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