Preparing Ectoderm/Endoderm Chimeras

  • Nancy Wanek


To produce chimeric hydra consisting of ectoderm from one strain and endoderm from a different strain. The procedure involves vital staining, grafting, and microsurgery. Nitrogen mustard-treated host animals are used to support the growth of small chimeras.


Host Animal Nitrogen Mustard Fish Line Glass Micropipet Vital Staining 
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  1. Campbell, R. D. 1974. Cell movements in Hydra. Am, Zool, 14:523–535Google Scholar
  2. Macklin, M. 1976. The effect of urethan on hydra. Biol. Bull. 150:442–452Google Scholar
  3. Wanek, N. and Campbell, R D. 1982. Roles of ectodermal and endodermal epithelial cells in hydra rnorphogenesis: construction of chimeric strains.J. Exp. Zool. 221:37–47.Google Scholar

Copyright information

© Springer Science+Business Media New York 1983

Authors and Affiliations

  • Nancy Wanek
    • 1
  1. 1.Developmental Biology Center and Department of Developmental and Cell BiologyUniversity of CaliforniaIrvineUSA

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