Culturing Interstitial Stem Cells in Hydra Aggregates
To investigate the growth and differentiation of interstitial stem cells of hydra, we have developed a method for culturing those cells within aggregates of cells taken from hydra previously treated with nitrogen mustard (NM) (David and Murphy, 1977; Sproull and David, 1979).
KeywordsPolyethylene Streptomycin Toluidine Baca
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- Diehl, F., and Burnett, A. L. 1964. The role of interstitial cells in the maintenance of hydra. 1. Specific destruction of interstititial cells in normal, sexual and non-budding animals./. Exp. Zoo1.155:253–259.Google Scholar
- Fujisawa, T., and David, C. N. 1981. Commitment during nematocyte differentiation in hydra. J. Cell Sci. 48:207–222Google Scholar
- Sproull, R, and David, C. N. 1979. Stem cell growth and differentiation in Hydra attenuata. I, Regulation of self-renewal probability in multi-clone aggregates. J. Cell Sei. 38. 155–169.Google Scholar
- Venugopal, G., and David, C. N. 1981. Nerve commitment in hydra. I. Role of morphogenetic signals. Der. Biol. 83: 353–360.Google Scholar
- Yaross, M., Baca, B., and Bode, H. 1982. Commitment of hydra interstitial cells to nerve cell differentiation must be initiated during S-phase. Der. Biol. (in press).Google Scholar