Fractionating Small Amounts of Radioactive Tissue

  • Howard M. Lenhoff

Abstract

Following the administration of a labeled substance to hydra, it may be necessary to determine the metabolic fate of that substance kinetically. I describe here two methods which make use of microfilters to fractionate labeled hydra. One uses a combination of centrifugation and microfilters to fractionate as few as 5–10 hydra. The second uses only microfilters during the separation procedures. I place particular emphasis on methods fractionating for proteins, nucleic acids, or carbohydrates.

Keywords

Sugar Filtration Carbohydrate Fractionation Boiling 

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References

  1. Lenhoff, H. M. 1961. Digestion of protein in hydra as studied using radicautoaraphy and fractionation by differential solubilities. Exp. Cell Res. 2: 335–353.CrossRefGoogle Scholar
  2. Lenhoff, H. M., and Roffman, B. 1971. Two methods for fractionating small amounts of radioactive tissue. In: Experimental Coelenterate Biology ( H. M. Lenhoff, L. Muscatine, and L. V. Davis, eds.), University of Hawaii Press, Honolulu, pp. 265–271.Google Scholar
  3. Roberts, R. Abelson, P., Cowie, D., Bolton, E., and Britten. R 1957. Studies of Biosynthesis in Escherichia Coli, Carnegie Institute of Washington Publication 607, Washington, D. C.Google Scholar

Copyright information

© Springer Science+Business Media New York 1983

Authors and Affiliations

  • Howard M. Lenhoff
    • 1
  1. 1.Department of Developmental and Cell BiologyUniversity of CaliforniaIrvineUSA

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