Labeling with Gaseous 14CO2 or by Feeding on Radioactive Tissues

  • Howard M. Lenhoff


To label hydra directly by feeding them on tissues that have been previously made radioactive or by exposing them to 14CO2, The ingested radioactive tissue labels the endoderm first and, within a day, most of the hydra’s cells (Lenhoff, 1961). The labeled 14CO2 passes directly into hydra and labels most intensely the interstitial cells and their derivative cells (Lenhoff, 1959); in the case of green hydra, the 14CO2 labels the endosymbiotic algae most intensely (Muscatine and Lenhoff, 1965).


Radioactive Waste Basal Disk Radioactive Tissue Plastic Test Tube Label Tissue 


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  1. Cowie, D. B., Bolton, E. T., and Sands, M. K. 1952. The labeling of bacterial cells with S35 For the production of high specific activity compounds. Arch. Biochem. Biophys. 35:140–145Google Scholar
  2. Lenhoff, H. M. 1959. Migration of 14C-labeled cnidoblasts. Exp. Cell Res. 17: 570–573.CrossRefGoogle Scholar
  3. Lenhoff, H. M. 1961. Digestion of protein in hydra as studied using radioautography and fractionation by differential solubilities. Exp. Cell Res. 23:335–353Google Scholar
  4. Muscatine, L., and Lenhoff, H. M. 1963. Symbiosis: On the role of algae symbiotic with hydra. Science 142: 956–958.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1983

Authors and Affiliations

  • Howard M. Lenhoff
    • 1
  1. 1.Department of Developmental and Cell BiologyUniversity of CaliforniaIrvineUSA

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