Advertisement

Vital Staining: Fluorescent and Immunofluorescent, and Review of Nonfluorescent Dyes

  • John F. Dunne
  • C. Lynne Littlefield

Abstract

To stain cells and tissues of live hydra with fluorescent dyes or by immunofluorescence. Such methods aid in identifying specific tissues or cells in grafts or in aggregates of cells.

Keywords

Evans Blue Vital Staining Aggregation Solution Hydra Tissue Hydra Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Gierer, A., Berking, S.Bode, H., David, C.N., Flick, K., Hansmann, G., Schaller, H., and Trenkner, E. 1972. Regeneration of hydra from reaggregated cells. Nature (London) 239:98–101.Google Scholar
  2. Klimek, F. 1979. Untersuchung zur Separation und Aggregation von Hydrazallen.Ph.D.thesis. Eberhard-Karis University. Tubingen.Google Scholar
  3. Mueller, W., and Gautier, F. 1975. Intercalation of heteroarornatic compounds with nucleic acids; A-T specific nonintercalating DNA ligands. Eur. J. Biochem.54:385.Google Scholar
  4. Rella. M. 1941. Vitalfarbungsuntersuchungen an Süsswasserhydrozoen. Proloplasma 35:298–317.Google Scholar
  5. Russell, W.C, Newman, C, and Williamson, D.a. (1975). A simple cytochemical technique for demonstration of DNA in cells infected with mycoplasrnas and viruses. Nature (London) 253: 461–462.CrossRefGoogle Scholar
  6. Schlottke, E. 1932. Zellstudien an Hydra, III. Versuche mit Vitalfarben. Z. Mikrosk. Anat. Forsch.28:298–336.Google Scholar
  7. Stidwill, R. 1981. Interspezifische Immmrati»Uitxmn zwischen Arten der Gattung Hydra (H,mnzoa,comuma) `Px.D. thesis, University of Zürich.Google Scholar
  8. Sugiyama, T., and Fujisawa, T. 1978. Genetic analysis of developmental mechanisms in hydra. II. Isolation and characterization of an interstitial cell-deficient straïn, J. Cell Sci. 29: 35–52.Google Scholar
  9. Trembley, A. 1744. Mémoires pour servir u l’histoire naturelle d’un genre de polype d’eau douce uom, en forme de comrs. Leyden.Google Scholar
  10. van der Kooy, D., and Kuypers, H. G. J. M. 1979. Fluorescent retrograde double labeling:Google Scholar
  11. Axonal branching in the ascending raphe and nigral projections. Science 204:873–875.Google Scholar
  12. Wanek, N. L. 1980. Roles of ectodermal and endodermal epithelial cells in hydra morphology genesis, Pd. D. thesis, University of Zürich.Google Scholar
  13. Weimer, B. R. 1972 . The use of Nile Blue sulfate as a vital stain on Hydra. Biol. Bull. 52:219–222.Google Scholar
  14. Wilby, O. K., and Webster, G. 1970. Experimental studies on axial polarity in Hydra. J. Embryol. Exp. Morphol. 24:495–613.Google Scholar
  15. Zoja. R. 1892. Die vitale Methylenblaufarbung bei Hydra. Zool. Anz. 15: 241–242Google Scholar

Copyright information

© Springer Science+Business Media New York 1983

Authors and Affiliations

  • John F. Dunne
    • 1
  • C. Lynne Littlefield
    • 1
  1. 1.Developmental Biology CenterUniversity of CaliforniaIrvineUSA

Personalised recommendations