Whole Mounts for Light Microscopy

  • Richard D. Campbell


To prepare whole mounts, thereby enabling one to study large areas of hydra in a single microscopic field in which spatial relationships are preserved between cells, and also to preserve experimental animals for future microscopic analyses.


Methylene Blue Toluidine Blue Cover Glass Interstitial Cell Basic Fuchsin 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Burnett, A. L. 1959. Histophysiology of growth in hydra. J. Exp. Zool, 140:281–342Google Scholar
  2. Burnett, A. L. 1960, The nematocyst of hydra. H. The maturation of nematocysts in hydra. Arm, Soc. R. Zool. Belg. 90: 269–280.Google Scholar
  3. Campbell, R. D., and Marcum, B. A. 1980. Nematocyte migration in hydra: Evidence for contact guidance in vivo. J. Cell. Sci. 41: 33–51.Google Scholar
  4. David, C. N., and Challoner, D. 1974. Distribution of interstitial cells and differentiating nematocysts in nests in Hydra attenuata. Am. Zool. 14: 537–542.Google Scholar
  5. Diehl, F. A., and Burnett, A. L. 1964. The role of interstitial cells in the maintenance of hydra. I. Specific destruction of interstitial cells in normal, asexual, and non-budding animals. J. Exp. Zool. 155: 253–260.PubMedCrossRefGoogle Scholar
  6. Otto, J. J. 1977. Orientation and behavior of epithelial cell muscle processes during hydra budding. J. Exp. Zool. 202: 307–322.PubMedCrossRefGoogle Scholar
  7. Zumstein, A., and Tardent, P. 1972. Autoradiography of whole-mounts of isolated ectoderm of the body column of hydra. A new method. Experientia 28: 1124–1125.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1983

Authors and Affiliations

  • Richard D. Campbell
    • 1
  1. 1.Department of Developmental and Cell BiologyUniversity of CaliforniaIrvineUSA

Personalised recommendations