Separated Somatotrophs: Their Use in Vitro and in Vivo

  • W. C. Hymer
  • R. Page
  • R. C. Kelsey
  • E. C. Augustine
  • W. Wilfinger
  • M. Ciolkosz
Part of the Biochemical Endocrinology book series (BIOEND)


Since the original discovery of growth hormone (GH) by Drs. Herbert Evans and Joseph Long in 1921, this molecule has been a subject of keen interest to biochemical endocrinologists. There are a number of reasons for this interest. To state but a few: (1) the molecule does not have a single, well-defined target organ, and its biological and metabolic effects are quite numerous (e.g., regulation of growth as well as metabolism of protein, carbohydrate, and fat); (2) given the availability of several assay systems, the molecule is well suited to structure-function studies; (3) the molecule has an established clinical usefulness for the treatment of hypopituitary children in addition to its suggested use in the treatment of ulcers, muscular dystrophy, and coronary-prone hypercholesterolemic patients (Li, 1975); and (4) the cell that produces GH, viz., the pituitary somatotroph, offers an interesting model for investigation of mechanisms of intracellular processing and secretion of peptide hormones in addition to the study of the regulation of somatotroph function. This chapter deals with the last issue.


Growth Hormone Hollow Fiber Pituitary Cell Disperse Cell Growth Hormone Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. Bancroft, F. C., Levine, L., and Tashjian, A., 1969, Control of growth hormone production by a clonal strain of rat pituitary cells, J. Cell Biol. 43: 432.PubMedCrossRefGoogle Scholar
  2. Bennett, L., 1975, Endocrinology and Herbert M. Evans, in: Hormonal Proteins and Peptides, Vol. III (C. H. Li, ed.), pp. 247–272, Academic Press, New York.Google Scholar
  3. Brazeau, P., Vale, W., Bengus, R., Ling, N., Butcher, M., Rivier, J., and Guillemin, R., 1973, Hypothalamic peptide that inhibits the secretion of immunoreactive pituitary growth hormone, Science 179: 77.PubMedCrossRefGoogle Scholar
  4. Daughaday, W., 1975, Regulation of growth by endocrines, Annu. Rev. Physiol. 37: 211.PubMedCrossRefGoogle Scholar
  5. Denef, C., Hautekeete, E., and Dewals, R., 1978, Monolayer cultures of gonadotrophs separated by velocity sedimentation: Heterogeneity in response to luteinizing hormone-releasing hormone, Endocrinology 103: 736.PubMedCrossRefGoogle Scholar
  6. Ellis, S., and Grindeland, R. E., 1974, in: Advances in Human Growth Hormone Research (S. Raiti, ed.), DHEW Publ. No. 74-612, pp. 409–424.Google Scholar
  7. Farquhar, M. G., Skutelsky, E. H., and Hopkins, C. R., 1975, Structure and function of the anterior pituitary and dispersed pituitary cells: In vitro studies, in: The Anterior Pituitary (A. Tixier-Vidal and M. G. Farquhar, eds.), pp. 84–135, Academic Press, New York.Google Scholar
  8. Garland, J., and Daughaday, W., 1972, Feedback inhibition of pituitary GH in rats infected with Spirometia, Proc. Soc. Exp. Biol. Med. 139: 497.Google Scholar
  9. Gittes, R. F., and Kastin, A. J., 1966, Effects of increasing numbers of pituitary transplants in hypophysectomized rats, Endocrinology 78: 1023.PubMedCrossRefGoogle Scholar
  10. Halasz, B., Pupp, L., Vhalrik, S., and Tima, L., 1963, Growth of hypophysectomized rats bearing pituitary transplant in the hypothalamus, Acta Physiol. Acad. Sci. Hung. 23: 287.PubMedGoogle Scholar
  11. Hymer, W. C., 1975, Separation of organelles and cells from the mammalian adenohypophysis, in: The Anterior Pituitary (A. Tixier-Vidal and M. G. Farquharr eds.), pp. 137–180, Academic Press, New York.Google Scholar
  12. Hymer, W. C., Kraicer, J., Bencosme, S., and Haskill, J., 1972, Purification of somato-trophs from the rat adenohypophysis by velocity and density gradient centrifugation, Proc. Soc. Exp. Biol. Med. 141: 966.PubMedGoogle Scholar
  13. Hymer, W. C., Snyder, J., Wilfinger, W., Swanson, N., and Davis, J., 1974, Separation of pituitary mammotrophs from the female rat by velocity sedimentation at unit gravity, Endocrinology 95: 107.PubMedCrossRefGoogle Scholar
  14. Hymer, W. C., Snyder, J., Wilfinger, W., Bergland, R., Fisher, B., and Pearson, O., 1976, Characterization of mammotrophs separated from the human pituitary gland, J. Natl. Cancer Inst. 57: 995.PubMedGoogle Scholar
  15. Köhler, P. O., Bridson, W., and Rayford, P. L., 1968, Cortisol stimulation of growth hormone production by monkey adenohypophysis in tissue culture, Biochem. Biophys. Res. Commun. 33: 834.PubMedCrossRefGoogle Scholar
  16. Kraicer, J., and Hymer, W. C., 1974, Purified somatotrophs from rat adenohypophysis: Response to secretagogues, Endocrinology 94: 1525.PubMedCrossRefGoogle Scholar
  17. Leathern, J., 1977, Hypophysectomy and Phillip E. Smith, in: Hormonal Proteins and Peptides, Vol. IV (C. H. Li, ed.), pp. 175–192, Academic Press, New York.Google Scholar
  18. Leuschen, M. P., Tobin, R., and Moriarty, M., 1978, Enriched populations of rat pituitary thyrotrophs in monolayer culture, Endocrinology 102: 509.PubMedCrossRefGoogle Scholar
  19. Li, C. H., 1975, The chemistry of human pituitary growth hormone: 1967–1973, in: Hormonal Proteins and Peptides, Vol. III (C. H. Li, ed.), pp. 1–40, Academic Press, New York.Google Scholar
  20. Li, C. H., 1977, Bioassay of pituitary growth hormone, in: Hormonal Proteins and Peptides, Vol. IV (C. H. Li, ed.), pp. 1–41, Academic Press, New York.Google Scholar
  21. Lowry, P. J., 1974, A sensitive method for the detection of corticotropin releasing factor using a perfused pituitary cell column, J. Endocrinol. 62: 163.PubMedCrossRefGoogle Scholar
  22. Miller, R., and Phillips, R., 1969, Separation of cells by velocity sedimentation, J. Cell. Physiol. 73: 191.PubMedCrossRefGoogle Scholar
  23. Milligan, J., Kraicer, J., Fawcett, C. P., and Illner, P., 1972, Purified growth hormone releasing factor increases 45Ca uptake into pituitary cells, Can. J. Physiol. Pharmacol. 50: 613.PubMedCrossRefGoogle Scholar
  24. Nikitovitch-Winer, M., and Everett, J. W., 1958, Functional restitution of pituitary grafts retransplanted from kidney to median eminence, Endocrinology 63: 916.PubMedCrossRefGoogle Scholar
  25. Peterson, E., and Evans, W., 1967, Separation of bone marrow cells by sedimentation at unit gravity, Nature (London) 214: 824.CrossRefGoogle Scholar
  26. Saffran, M., and Schally, A. V., 1955, The release of corticotropin by anterior pituitary tissue in vitro, Can. J. Biochem. 33: 408.PubMedCrossRefGoogle Scholar
  27. Severinghaus, A. E., 1937, Cellular changes in the anterior hypophysis with special reference to its secretory activities, Physiol. Rev. 17: 556.Google Scholar
  28. Shiino, M., Ishikawa, H., and Renneis, E., 1977, In vitro and in vivo studies on cytodifferentiation of pituitary clonal cells derived from epithelium of Rathke’s pouch, Cell Tissue Res. 181: 473.PubMedCrossRefGoogle Scholar
  29. Shortman, K., 1968, The separation of different cell classes from lymphoid organs, Aust. J. Exp. Biol. Med. Sci. 46: 375.PubMedCrossRefGoogle Scholar
  30. Smith, P. E., 1963, Postponed pituitary homotransplants into the region of the hypophysial portal circulation in hypophysectomized female rats, Endocrinology 73: 793.PubMedCrossRefGoogle Scholar
  31. Snyder, G., and Hymer, W. C., 1975, A short method for the isolation of somatotrophs from the rat pituitary gland, Endocrinology 96: 792.PubMedCrossRefGoogle Scholar
  32. Snyder, G., Hymer, W. C., and Snyder, J., 1977, Functional heterogeneity in somatotrophs isolated from the rat anterior pituitary, Endocrinology 101: 788.PubMedCrossRefGoogle Scholar
  33. Snyder, J., Wilfinger, W., and Hymer, W. C., 1976, Maintenance of separated rat pituitary mammotrophs in cell culture, Endocrinology 98: 25.PubMedCrossRefGoogle Scholar
  34. Snyder, J., Hymer, W. C., and Wilfinger, W., 1978, Culture of human pituitary prolactin and growth hormone cells, Cell Tissue Res. 191: 379.PubMedCrossRefGoogle Scholar
  35. Stachura, M., 1976, Mechanism of somatostatin action: Influence of synthetic SRIF and dibutyryl cyclic AMP on GH release by rat pituitaries in vitro, Proc. Annu. Meet. Endocrinol. Soc. 58: A–133.Google Scholar
  36. Stachura, M., 1977, Interaction of somatostatin inhibition and dibutyryl cyclic AMP or potassium stimulation of growth hormone release from perfused rat pituitaries, Endocrinology 101: 1044.PubMedCrossRefGoogle Scholar
  37. Stachura, M., Szabo, M., and Frohman, L. A., 1978, Multiphasic effect of porcine stalk median eminence extract on growth hormone release from perfused rat pituitaries, Endocrinology 102: 1520.PubMedCrossRefGoogle Scholar
  38. Tannenbaum, G. S., and Martin, J. B., 1976, Evidence for an endogenous ultradian rhythm governing growth hormone secretion in the rat, Endocrinology 98: 562.PubMedCrossRefGoogle Scholar
  39. Tze, W., Wong, F., Chen, L., and O’Young, S., 1976, Implantable artificial endocrine pancreas unit used to restore normoglycemia in the diabetic rat, Nature (London) 264: 466.CrossRefGoogle Scholar
  40. Weiss, S., Bergland, R., Page, R., Turpen, C., and Hymer, W. C., 1978, Pituitary cell transplants in the cerebral ventricles promote growth of hypophysectomized rats, Proc. Soc. Exp. BioL Med. 159: 409.PubMedGoogle Scholar
  41. Wilfinger, W., David, J., Augustine, E. C., and Hymer, W. C., 1979, Effects of culture conditions on prolactin and growth hormone production by rat anterior pituitary cells, Endocrinology 105: 530.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1980

Authors and Affiliations

  • W. C. Hymer
    • 1
  • R. Page
    • 2
  • R. C. Kelsey
    • 3
  • E. C. Augustine
    • 1
  • W. Wilfinger
    • 4
  • M. Ciolkosz
    • 1
  1. 1.Department of Biochemistry and BiophysicsThe Pennsylvania State UniversityUniversity ParkUSA
  2. 2.Hershey Medical CenterHersheyUSA
  3. 3.East Stroudsburg State CollegeEast StroudsburgUSA
  4. 4.University of Cincinnati Medical SchoolCincinnatiUSA

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