TRH as MSH-Releasing Factor in the Frog

  • Marie-Christine Tonon
  • François Leboulenger
  • Catherine Delarue
  • Sylvie Jegou
  • Jean Fresel
  • Philippe Leroux
  • Hubert Vaudry
Part of the Biochemical Endocrinology book series (BIOEND)


The tripeptide pyroglutamyl-histidyl-prolinamide plays the role of thyro-tropin-releasing hormone (TRH) (Schally et al., 1973; Vale et al., 1973) and a prolactin-releasing hormone in mammals (Bowers et al., 1971; Rivier and Vale, 1974). The development of sensitive and highly specific radioim-munoassays (RIAs) (Jackson and Reichlin, 1974; Oliver et al., 1974) has made possible the assessment of TRH in various brain regions in mammals. The brain of submammalian vertebrates contains a peptide capable of releasing thyrotropin (TSH) from rat pituitary thyrotrophs in vivo (Jackson and Reichlin, 1974) and in vitro (Taurog et al., 1974). Concurrently, on the basis of electrophoretic comigration (Taurog et al., 1974) and gel filtration (Jackson and Reichlin, 1974), it was established that a peptide, chemically related to mammalian TRH, was present in the brain of amphibians. These results were confirmed by radioimmunological data that showed a strict parallelism between the competitive inhibition curves obtained with synthetic TRH and frog brain extracts (Taurog et al., 1974; Jackson and Reichlin, 1974).


Frog Skin Perfusion Chamber ACTH Release Intermediate Lobe Melanocyte Stimulate Hormone 
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Copyright information

© Springer Science+Business Media New York 1980

Authors and Affiliations

  • Marie-Christine Tonon
    • 1
  • François Leboulenger
    • 1
  • Catherine Delarue
    • 1
  • Sylvie Jegou
    • 1
  • Jean Fresel
    • 1
  • Philippe Leroux
    • 1
  • Hubert Vaudry
    • 1
  1. 1.Groupe de Recherche en Endocrinologie Moléculaire, Laboratoire d’Endocrinologie, Institut de Biochimie et Physiologie Cellulaire, Faculté des SciencesUniversité de Haute-NormandieMont-Saint-AignanFrance

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